Frozen Environments and Soft Tissue Preservation

Micozzi, Marc S.

doi:10.1201/9781439821923.ch11

Cited by 22 publications

(31 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes can be further potentiated by thawing [13,17]. This methodology allowed circumventing the issue of specimen conservation, by assuring that all specimens were used immediately after acquisition.…”

Section: Discussionmentioning

confidence: 99%

Timing of blunt force injuries in long bones: The effects of the environment, PMI length and human surrogate model

Coelho¹,

Cardoso²

2013

Forensic Science International

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Timing of blunt force injuries in long bones: The effects of the environment, PMI length and human surrogate model

Coelho¹,

Cardoso²

2013

Forensic Science International

View full text Add to dashboard Cite

show abstract

“…Alteration of the ecosystem, such as exclusion of insects or burial, may lead to a unique trajectory for decomposition and potentially anomalous results [2][3][4]; therefore, it is critical to forensics that the interplay of ecological factors be understood. However, despite possible variation, certain patterns exist.…”

Section: Introductionmentioning

confidence: 99%

“…Investigation of patterns of microbial community succession may lead to additional tools available for forensic researchers. Organisms that interact with cadavers are a product of factors such as geographic region, season, weather, and conditions of the cadaver [3,8,9]. A comprehensive database of organisms associated with cadavers in conjunction with seasonality and accumulated degree days to which a cadaver has been exposed for a particular geographical area can be vital information for criminal investigations if these organisms can be shown to colonize cadavers in a predictable fashion (as with insects and cadavers [2,[13][14][15][16]).…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria are credited as a major driving force for the process of decomposition with numerous studies attempting to catalog the microbiome of decomposition [3,11,12,[17][18][19][20][21][22][23][24][25][26]. However, most of these studies have relied on culture-dependent techniques, which can limit the number of microorganisms documented.…”

Section: Introductionmentioning

confidence: 99%

“…Purge is associated with an opening of the abdominal cavity to the environment [11], at which point, the rate of decay may greatly increase as larval flies remove large portions of tissues [2,[13][14][15][16]. The final stages of decomposition last through to complete skeletonization and are the driest stages [2,3,9,17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Initial insights into bacterial succession during human decomposition

et al. 2014

View full text Add to dashboard Cite

Decomposition is a dynamic ecological process dependent upon many factors such as environment, climate, and bacterial, insect, and vertebrate activity in addition to intrinsic properties inherent to individual cadavers. Although largely attributed to microbial metabolism, very little is known about the bacterial basis of human decomposition. To assess the change in bacterial community structure through time, bacterial samples were collected from several sites across two cadavers placed outdoors to decompose and analyzed through 454 pyrosequencing and analysis of variable regions 3-5 of the bacterial 16S ribosomal RNA (16S rRNA) gene. Each cadaver was characterized by a change in bacterial community structure for all sites sampled as time, and decomposition, progressed. Bacteria community structure is variable at placement and before purge for all body sites. At bloat and purge and until tissues began to dehydrate or were removed, bacteria associated with flies, such as Ignatzschineria and Wohlfahrtimonas, were common. After dehydration and skeletonization, bacteria associated with soil, such as Acinetobacter, were common at most body sites sampled. However, more cadavers sampled through multiple seasons are necessary to assess major trends in bacterial succession.

show abstract

The development of a post-mortem interval estimation for human remains found on land in the Netherlands

et al. 2017

View full text Add to dashboard Cite

The decomposition process of human remains can be used to estimate the post-mortem interval (PMI), but decomposition varies due to many factors. Temperature is believed to be the most important and can be connected to decomposition by using the accumulated degree days (ADD). The aim of this research was to develop a decomposition scoring method and to develop a formula to estimate the PMI by using the developed decomposition scoring method and ADD.A decomposition scoring method and a Book of Reference (visual resource) were made. Ninety-one cases were used to develop a method to estimate the PMI. The photographs were scored using the decomposition scoring method. The temperature data was provided by the Royal Netherlands Meteorological Institute. The PMI was estimated using the total decomposition score (TDS) and using the TDS and ADD. The latter required an additional step, namely to calculate the ADD from the finding date back until the predicted day of death.The developed decomposition scoring method had a high interrater reliability. The TDS significantly estimates the PMI (R 2 = 0.67 and 0.80 for indoor and outdoor bodies, respectively). When using the ADD, the R 2 decreased to 0.66 and 0.56.The developed decomposition scoring method is a practical method to measure decomposition for human remains found on land. The PMI can be estimated using this method, but caution is advised in cases with a long PMI. The ADD does not account for all the heat present in a decomposing remain and is therefore a possible bias.Electronic supplementary materialThe online version of this article (10.1007/s00414-017-1700-9) contains supplementary material, which is available to authorized users.

show abstract

Frozen Environments and Soft Tissue Preservation

Cited by 22 publications

References 9 publications

Timing of blunt force injuries in long bones: The effects of the environment, PMI length and human surrogate model

Timing of blunt force injuries in long bones: The effects of the environment, PMI length and human surrogate model

Initial insights into bacterial succession during human decomposition

The development of a post-mortem interval estimation for human remains found on land in the Netherlands

Contact Info

Product

Resources

About