Frozen‐and‐thawed allogeneic platelet gels for treating postoperative chronic wounds

Perseghin, Paolo; Sciorelli, G; Belotti, Daniela; Speranza, Tiziana; Pogliani, Enrico Maria; Ferro, Orazio; Gianoli, Monica; Porta, Fabiano; Paolini, Giovanni

doi:10.1111/j.1537-2995.2005.00570.x

Cited by 12 publications

(11 citation statements)

References 5 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This in turn indicated that either CB or APB PLT derived products can act in the same way, without any significant alteration to their therapeutic result. Indeed, Tadini et al [23], Janmey et al [7] and Perseghin et al [24] have confirmed the beneficial effect of CB derived fibrin gel and PRP in the treatment of skin lesions produced by dystrophic epidermolysis bullosa and chronic wounds. Furthermore, the therapeutic properties of fibrin gel may be useful in bone and muscle tissue regeneration.…”

Section: Discussionmentioning

confidence: 97%

Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study

et al. 2019

View full text Add to dashboard Cite

Background: Recent findings have shown that the fibrin gel derived from cord blood units (CBUs) play a significant role in wound healing and tissue regeneration. The aim of this study was to standardize the fibrin gel production process in order to allow for its regular use. Methods: CBUs (n = 200) were assigned to 4 groups according to their initial volume. Then, a two-stage centrifugation protocol was applied in order to obtain platelet rich plasma (PRP). The concentration of platelets (PLTs), white blood cells (WBCs) and red blood cells (RBCs) were determined prior to and after the production process. In addition, targeted proteomic analysis using multiple reaction monitoring was performed. Finally, an appropriate volume of calcium gluconate was used in PRP for the production of fibrin gel. Results: The results of this study showed that high volume CBUs were characterized by greater recovery rates, concentration and number of PLTs compared to the low volume CBUs. Proteomic analysis revealed the presence of key proteins for regenerative medicine. Fibrin gel was successfully produced from CBUs of all groups. Conclusion: In this study, low volume CBUs could be an alternative source for the production of fibrin gel, which can be used in multiple regenerative medicine approaches.

show abstract

Section: Discussionmentioning

confidence: 97%

Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Many previous studies have reported that PLs can stimulate the proliferation of various cells, for example, osteoblast, gingival fibroblast, endothelial cells, and stromal stem cells. [16][17][18][19][20] Most of these studies were carried out in twodimensional culture systems. In this study, ACL cells were cultured in 3D collagen type I hydrogel, and no significant difference of cell proliferation was observed among all the four groups after 14 days culture.…”

Section: Discussionmentioning

confidence: 99%

Platelets and Plasma Proteins Are Both Required to Stimulate Collagen Gene Expression by Anterior Cruciate Ligament Cells in Three-Dimensional Culture

Cheng

Wang

Yoshida

et al. 2010

Tissue Engineering Part A

View full text Add to dashboard Cite

Collagen-platelet (PL)-rich plasma composites have shown in vivo potential to stimulate anterior cruciate ligament (ACL) healing at early time points in large animal models. However, little is known about the cellular mechanisms by which the plasma component of these composites may stimulate healing. We hypothesized that the components of PL-rich plasma (PRP), namely the PLs and PL-poor plasma (PPP), would independently significantly influence ACL cell viability and metabolic activity, including collagen gene expression. To test this hypothesis, ACL cells were cultured in a collagen type I hydrogel with PLs, PPP, or the combination of the two (PRP) for 14 days. The inclusion of PLs, PPP, and PRP all significantly reduced the rate of cell apoptosis and enhanced the metabolic activity of fibroblasts in the collagen hydrogel. PLs promoted fibroblast-mediated collagen scaffold contraction, whereas PPP inhibited this contraction. PPP and PRP both promoted cell elongation and the formation of wavy fibrous structure in the scaffolds. The addition of only PLs or only plasma proteins did not significantly enhance gene expression of collagen types I and III but the combination, as PRP, did. Our findings suggest that the addition of both PLs and plasma proteins to collagen hydrogel may be useful in stimulating ACL healing by enhancing ACL cell viability, metabolic activity, and collagen synthesis.

show abstract

“…PLT-gels and PRP contain high a concentration of platelet-derived growth factors, transforming growth factor-β1, and vascular endothelial growth factor which enhances activity of fibroblasts, mesenchymal cells, and stromal cells in the process of wound healing. 120 It has also been observed that PRP in vitro inhibits the growth of S. aureus and E. coli. 121 Cold atmospheric plasma (CAP) as a novel treatment of DLI has been described by Hilker et al 122 CAP causes the formation of reactive oxygen and nitrogen species that affect the cellular redox balance and can effectively inactivate microorganisms.…”

Section: Treatmentmentioning

confidence: 99%

Ventricular Assist Device Driveline Infections: A Systematic Review

Krželj

Petričević

Gašparović

et al. 2021

Thorac Cardiovasc Surg

View full text Add to dashboard Cite

Infection is the most common complication in patients undergoing ventricular assist device (VAD) implantation. Driveline exit site (DLES) infection is the most frequent VAD infection and is a significant cause of adverse events in VAD patients, contributing to morbidity, even mortality, and repetitive hospital readmissions. There are many risk factors for driveline infection (DLI) including younger age, smaller constitution of patients, obesity, exposed velour at the DLES, longer duration of device support, lower cardiac index, higher heart failure score, DLES trauma, and comorbidities such as diabetes mellitus, chronic kidney disease, and depression. The incidence of DLI depends also on the device type. Numerous measures to prevent DLI currently exist. Some of them are proven, whereas the others remain controversial. Current recommendations on DLES care and DLI management are predominantly based on expert consensus and clinical experience of the certain centers. However, careful and uniform DLES care including obligatory driveline immobilization, previously prepared sterile dressing change kits, and continuous patient education are probably crucial for prevention of DLI. Diagnosis and treatment of DLI are often challenging because of certain immunological alterations in VAD patients and microbial biofilm formation on the driveline surface areas. Although there are many conservative and surgical methods described in the DLI treatment, the only possible permanent solution for DLI resolution in VAD patients is heart transplantation. This systematic review brings a comprehensive synthesis of recent data on the prevention, diagnostic workup, and conservative and surgical management of DLI in VAD patients.

show abstract

Frozen‐and‐thawed allogeneic platelet gels for treating postoperative chronic wounds

Cited by 12 publications

References 5 publications

Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study

Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study

Platelets and Plasma Proteins Are Both Required to Stimulate Collagen Gene Expression by Anterior Cruciate Ligament Cells in Three-Dimensional Culture

Ventricular Assist Device Driveline Infections: A Systematic Review

Contact Info

Product

Resources

About