Frontispiece: Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly, Michelle D.; Martin, Lisandra L.; Thang, San H.

doi:10.1002/chem.202185161

Cited by 2 publications

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“…Historically, the only agents available for membrane protein manipulation were a limited number of detergents with relatively simple architectures, which in turn limited the number and type of membrane proteins that could be worked with. Recent tools and developments detailed in the sections below (novel detergents, [5] nanodiscs, [6,7] amphipols, [8] polymers [9] ) have made research in this area much more accessible meaning that researchers can now explore a greater range of membrane proteins and, excitingly, membrane protein complexes. Most importantly, we are starting to see the first structures of proteins in native membranes demonstrating what has to be the true gold standard of analysis, which in the future holds the potential to circumvent the use of membrane mimetics for certain downstream analyses.…”

Section: Introductionmentioning

confidence: 99%

Advances in Membrane Mimetic Systems for Manipulation and Analysis of Membrane Proteins: Detergents, Polymers, Lipids and Scaffolds

Woubshete,

Cioccolo,

Byrne

2024

ChemPlusChem

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Extracting membrane proteins from the hydrophobic environment of the biological membrane, in a physiologically relevant and stable state, suitable for downstream analysis remains a challenge. The traditional route to membrane protein extraction has been to use detergents and the last 15 years or so have seen a veritable explosion in the development of novel detergents with improved properties, making them more suitable for individual proteins and specific applications. There have also been significant advances in the development of encapsulation of membrane proteins in lipid based nanodiscs, either directly from the native membrane using polymers allowing effective capture of the protein and protein‐associated membrane lipids, or via reconstitution of detergent extracted and purified protein into nanodiscs of defined lipid composition. All of these advances have been successfully applied to the study of membrane proteins via a range of techniques and there have been some spectacular membrane protein structures solved using these new approaches. In addition, the first detailed structural and biophysical analyses of membrane proteins retained within a biological membrane have been reported. Here we summarise and review the recent advances with respect to these new agents and systems for membrane protein extraction, reconstitution and analysis.

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Section: Introductionmentioning

confidence: 99%

Advances in Membrane Mimetic Systems for Manipulation and Analysis of Membrane Proteins: Detergents, Polymers, Lipids and Scaffolds

Woubshete,

Cioccolo,

Byrne

2024

ChemPlusChem

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Insights into the Role of Membrane Lipids in the Structure, Function and Regulation of Integral Membrane Proteins

Renard

Byrne

2021

IJMS

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Membrane proteins exist within the highly hydrophobic membranes surrounding cells and organelles, playing key roles in cellular function. It is becoming increasingly clear that the membrane does not just act as an appropriate environment for these proteins, but that the lipids that make up these membranes are essential for membrane protein structure and function. Recent technological advances in cryogenic electron microscopy and in advanced mass spectrometry methods, as well as the development of alternative membrane mimetic systems, have allowed experimental study of membrane protein–lipid complexes. These have been complemented by computational approaches, exploiting the ability of Molecular Dynamics simulations to allow exploration of membrane protein conformational changes in membranes with a defined lipid content. These studies have revealed the importance of lipids in stabilising the oligomeric forms of membrane proteins, mediating protein–protein interactions, maintaining a specific conformational state of a membrane protein and activity. Here we review some of the key recent advances in the field of membrane protein–lipid studies, with major emphasis on respiratory complexes, transporters, channels and G-protein coupled receptors.

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Frontispiece: Polymer Nanodiscs and Their Bioanalytical Potential

Cited by 2 publications

References 0 publications

Advances in Membrane Mimetic Systems for Manipulation and Analysis of Membrane Proteins: Detergents, Polymers, Lipids and Scaffolds

Advances in Membrane Mimetic Systems for Manipulation and Analysis of Membrane Proteins: Detergents, Polymers, Lipids and Scaffolds

Insights into the Role of Membrane Lipids in the Structure, Function and Regulation of Integral Membrane Proteins

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