Frontiers of transcutaneous vaccination systems: Novel technologies and devices for vaccine delivery

Matsuo, Keitaro; Hirobe, Sachiko; Okada, Naoki; Nakagawa, Shinsaku

doi:10.1016/j.vaccine.2013.03.022

Cited by 51 publications

(31 citation statements)

References 94 publications

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“…Activation of APCs immensely enhances their ability to capture antigen, and thereby elicit an antigenspecific immune response. [26][27][28] Seo et al studied effective immunization using activated LCs and addressed the augmented expression of major histocompatibility complex (MHC) class II and costimulatory molecules (CD86, CD80, CD54) on LCs, the enhanced cytokine activity, the increased cytotoxic T lymphocyte activity, and the T cell proliferation. Strong antigen-presenting capacity was obtained in activated LCs isolated from tape-stripped (TS) skin compared with LCs isolated from nonactivated intact skin.…”

mentioning

confidence: 99%

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Takeuchi

Nomoto

Watanabe

et al. 2016

Biological & Pharmaceutical Bulletin

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An adequate immune response to percutaneous vaccine application is generated by delivery of sufficient amounts of antigen to skin and by administration of toxin adjuvants or invasive skin abrasion that leads to an adjuvant effect. Microneedles penetrate the stratum corneum, the outermost layer of the skin, and enable direct delivery of vaccines from the surface into the skin, where immunocompetent dendritic cells are densely distributed. However, whether the application of microneedles to the skin activates antigen-presenting cells (APCs) has not been demonstrated. Here we aimed to demonstrate that microneedles may act as a potent physical adjuvant for successful transcutaneous immunization (TCI). We prepared samples of isolated epidermal and dermal cells and analyzed the expression of major histocompatibility complex (MHC) class II and costimulatory molecules on Langerhans or dermal dendritic cells in the prepared samples using flow cytometry. The expression of MHC class II and costimulatory molecules demonstrated an upward trend in APCs in the skin after the application of 500-and 300-µm microneedles. In addition, in the epidermal cells, application of microneedles induced more effective activation of Langerhans cells than did an invasive tapestripping (positive control). In conclusion, the use of microneedles is likely to have a positive effect not only as an antigen delivery system but also as a physical technique inducing an adjuvant-like effect for TCI.

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mentioning

confidence: 99%

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Takeuchi

Nomoto

Watanabe

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Treatment of common infectious diseases (such as influenza, rabies and tuberculosis) and many allergies often requires a regular dosage of vaccines 13,14 and allergens 15 in specific quantities at periodic times to have desired biological response.…”

Section: Importance Of Pulsatile Releasementioning

confidence: 99%

Barrier-Mediated Pulsatile Release

Gandhi

Gosse

Nishii

et al. 2015

Journal of Membrane Science

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ii To my loving wife and wonderful parents.iii ACKNOWLEDGMENTS First and foremost I would like to thank my research advisor Dr. Eric Nuxoll for his support, brilliant advice, professionalism and endless patience. He has been a great mentor throughout my doctoral studies at the University of Iowa since I became his first graduate student. Besides overall research skills, I learned much more including how to address a problem in a practical way, determine priorities and most importantly how to express and defend ideas. I have had the privilege to work with very talented undergrad students Matthew Gosse and Derek Baerenwald, who directly contributed to this project. I am also grateful to Yasuhiro Nishii, a visiting scholar from Japan, for his partial contributions to this work and continued work forward with this project. Guiding and mentoring these bright individuals was an exciting opportunity and also a great experience for me. Others who have helped me along the way include my fellow research group members Ann O'Toole, Joel Coffel, Erica Bader and Bryce Hundley. iv I am incredibly grateful to my parents who have always supported me. I have been blessed to be born in a loving family that has fully encouraged and enabled me to achieve my dreams. Finally, no words can express my thankfulness to Khushbu, my wife, who always stood by me throughout this journey. Without her encouragement and motivation I would not have pursued the education to the length I have.v ABSTRACT Solutes are often most efficiently deployed in discrete pulses, for example in the delivery of herbicides or drugs. Manual application of each pulse can be labor-intensive, automated application of each pulse can be capital intensive, and both are often costly and impractical. Barrier-Mediated Pulsatile Release (BMPR) systems offer a materialsbased alternative for automated pulsatile drug delivery, without pumps, power supplies, or complex circuitry. While earlier materials-based approaches such as delayed-release microcapsules are limited to two or three pulses due to the independent nature of each pulse's timing control, BMPR systems link the timing of each pulse to the previous pulse.Each dose of drug is sequestered in its own stimuli-sensitive depot, releasing only upon contact with the stimulant. These depots are stacked with sacrificial barriers in between, each of which block the stimulant for a predetermined time. For instance, layers of soluble drug may be separated by degradable polymer layers. Water, as the stimulant, will erode the polymer layer over a fixed period of time, followed by quick dissolution and release of the underlying drug and the start of degradation for the next polymer layer.This example, however, is quickly limited by irregular polymer erosion, a single stimulant (water), and difficulty in scaling delay times.The research work presented in this thesis reports the development of a generalized BMPR system which overcomes those limitations. Model drugs (methylene blue and methyl orange) were immobilized in a pH-sensitive ...

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“…One solution to this was the evolution of biocompatible and dissolving microneedles. 39 Matsuo et al looked at its delivery of vaccines and so a study around the delivery of botulinum toxin through dissolvable microneedles would be beneficial. 39 Another hurdle to overcome was to ensure that microneedles can pierce the skin in a reproducible manner.…”

Section: Microwave Thermolysismentioning

confidence: 99%

“…39 Matsuo et al looked at its delivery of vaccines and so a study around the delivery of botulinum toxin through dissolvable microneedles would be beneficial. 39 Another hurdle to overcome was to ensure that microneedles can pierce the skin in a reproducible manner. Verbaan et al assessed the ability of microneedles to pierce the skin using a manual or with an electrically driven applicator at speeds of 3 or 1 m/s.…”

Section: Microwave Thermolysismentioning

confidence: 99%

Axillary hyperhidrosis: A review of the extent of the problem and treatment modalities

2015

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Frontiers of transcutaneous vaccination systems: Novel technologies and devices for vaccine delivery

Cited by 51 publications

References 94 publications

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Barrier-Mediated Pulsatile Release

Axillary hyperhidrosis: A review of the extent of the problem and treatment modalities

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