Frontiers in Neurogenesis

Vaz, Andreia; Ribeiro, Inês; Pinto, Luísa

doi:10.3390/cells11223567

Cited by 7 publications

(4 citation statements)

References 58 publications

(58 reference statements)

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“…Neuroblasts play a significant role during embryonic development. However, they persist into adulthood, contributing to the generation of fresh brain cells and assisting in the recovery process following brain injury and neuronal death [ 25 ]. The neuronal cell death is accompanied by an increased unesterified cholesterol release and conversion of cholesterol into the polar metabolite; the present analysis concentrated on evaluating the defenses against oxidative stress, oxysterols, and unesterified cholesterol during the early stages of neuroblast proliferation [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effect of Solid Lipid Nanoparticles Loaded with Lepidium sativum (L.) Seed Bioactive Components Enhance Bioavailability and Wnt/β-Catenin/Camk-II Signaling Cascade in SH-SY5Y Neuroblastoma Cells

Al-Saran,

Subash-Babu,

Al-Harbi

et al. 2024

Nanomaterials

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The primary pathological hallmark of Alzheimer’s disease (AD) is the formation and accumulation of neurofibrillary tangles and plaques, which result from the aggregation of amyloid-β (Aβ) induced by oxidative stress. The effectiveness of Alzheimer’s disease (AD) therapeutics significantly hinges on the drug’s bioavailability and its ability to penetrate neuronal cells. The current investigation was designed as a first attempt to examine bio-fabricated Lepidium sativum (LS) seed-extract-loaded solid lipid nanoparticles (SLNps) to increase bioavailability and bioefficacy for the prevention of undifferentiated SH-SY5Y neuronal cells from oxidative stress induced by H2O2 and amyloid-β peptide (Aβ,1-42). The SLNps were fabricated using LS extract as a water phase and hyaluronic acid and chia seed fatty acids as a lipid phase, then confirmed and characterized using UV, Zeta size, and SEM methods. The biological safety of synthesized LS-SLNps has been determined using MTT assay and PI staining (nuclear damage) in hMSCs. LS-SLNp-pretreated neuronal cells were induced with oxidative stress and 2 µM of beta-amyloid (Aβ,1-42) fibrils; furthermore, the neuroprotective potential of LS-SLNps was determined through the quenching of oxidative stress, enhancing mitochondrial oxidative capacity, and immunoregulatory potential. Observations found that cells treated with both H2O2 and beta-amyloid (Aβ,1-42) fibrils showed decreased neuronal cell growth, nuclear damage, and mitochondrial membrane potential due to oxidative stress. However, SH-SY5Y cells pretreated with LS-SLNps for 24 h showed an increase in cell proliferation with uniform morphology and increased mitochondrial membrane potential compared to cells pretreated with LS alone. Gene expression analysis found that LS-SLNps increased the expression of Wnt 3a and 5a, which stimulated the canonical, β-catenin, and non-canonical Camk-II expressions of nerve cell growth factors, confirming the molecular-level reversal of neurodegenerative diseases.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Effect of Solid Lipid Nanoparticles Loaded with Lepidium sativum (L.) Seed Bioactive Components Enhance Bioavailability and Wnt/β-Catenin/Camk-II Signaling Cascade in SH-SY5Y Neuroblastoma Cells

Al-Saran,

Subash-Babu,

Al-Harbi

et al. 2024

Nanomaterials

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“…In vivo, stimulus-driven proliferation of NSCs and their conversion into NPCs may take up to 3 days, followed by the differentiation within 1 week and further development of mature neurons within 4–6 weeks. 237 There is a difference in the time-course of adult neurogenesis in two niches: development of newborn neurons in the SGZ takes more than1.5 month before their complete maturation and functional integration into the pre-existing neuronal circuits, whereas development of newborn neurons in the SVZ occurs within 1 month. 238 …”

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Salmina,

Alexandrova,

Averchuk

et al. 2024

J Tissue Eng

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In vitro modeling of brain tissue is a promising but not yet resolved problem in modern neurobiology and neuropharmacology. Complexity of the brain structure and diversity of cell-to-cell communication in (patho)physiological conditions make this task almost unachievable. However, establishment of novel in vitro brain models would ultimately lead to better understanding of development-associated or experience-driven brain plasticity, designing efficient approaches to restore aberrant brain functioning. The main goal of this review is to summarize the available data on methodological approaches that are currently in use, and to identify the most prospective trends in development of neurovascular unit, blood-brain barrier, blood-cerebrospinal fluid barrier, and neurogenic niche in vitro models. The manuscript focuses on the regulation of adult neurogenesis, cerebral microcirculation and fluids dynamics that should be reproduced in the in vitro 4D models to mimic brain development and its alterations in brain pathology. We discuss approaches that are critical for studying brain plasticity, deciphering the individual person-specific trajectory of brain development and aging, and testing new drug candidates in the in vitro models.

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“…In conclusion, a comprehensive review of Nissl granules, axonal regeneration, and their applications in regenerative therapeutics is poised at the intersection of neurobiology and regenerative medicine. This understanding holds promise for advancing therapeutic approaches that capitalize on the inherent reparative mechanisms within neuronal constituents, paving the way for innovative strategies in the realm of regenerative medicine [12][13][14][15][16]. Figure 2 shows the schematic diagram of axonal regeneration.…”

Section: Basic Physiology Behind Nissl Granules and Axonal Regenerationmentioning

confidence: 99%

Nissl Granules, Axonal Regeneration, and Regenerative Therapeutics: A Comprehensive Review

Bhati,

Thakre,

Anjankar

2023

Cureus

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Nissl granules, traditionally recognized for their pivotal role in protein synthesis within neuronal cell bodies, are emerging as intriguing components with far-reaching implications in the realm of regenerative therapeutics. This abstract encapsulates the essence of a comprehensive review, exploring the nexus between Nissl granules, axonal regeneration, and their transformative applications in regenerative medicine. The molecular intricacies of Nissl granules form the foundation of this exploration, unraveling their dynamic role in orchestrating cellular responses, particularly in the context of axonal regeneration. As we delve into the interplay between Nissl granules and regenerative processes, this review highlights the diverse mechanisms through which these granules contribute to neuronal repair and recovery. Beyond their conventional association with neurobiology, recent advancements underscore the translational potential of Nissl granules as therapeutic agents. Insights into their involvement in enhancing axonal regeneration prompt a reconsideration of these granules as key players in the broader field of regenerative medicine. The abstract encapsulates evidence suggesting that modulating Nissl granule-related pathways holds promise for augmenting tissue regeneration, extending their applicability beyond the confines of the nervous system. This review aims to serve as a valuable resource for medical professionals, researchers, and clinicians seeking to comprehend the multifaceted role of Nissl granules in regenerative therapeutics. By illuminating the intricate connections between Nissl granules, axonal regeneration, and therapeutic applications, this work aspires to catalyze further research and innovation, ultimately contributing to the evolution of regenerative strategies that harness the innate reparative capacities within cellular constituents.

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Frontiers in Neurogenesis

Cited by 7 publications

References 58 publications

Neuroprotective Effect of Solid Lipid Nanoparticles Loaded with Lepidium sativum (L.) Seed Bioactive Components Enhance Bioavailability and Wnt/β-Catenin/Camk-II Signaling Cascade in SH-SY5Y Neuroblastoma Cells

Neuroprotective Effect of Solid Lipid Nanoparticles Loaded with Lepidium sativum (L.) Seed Bioactive Components Enhance Bioavailability and Wnt/β-Catenin/Camk-II Signaling Cascade in SH-SY5Y Neuroblastoma Cells

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Nissl Granules, Axonal Regeneration, and Regenerative Therapeutics: A Comprehensive Review

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