Frontiers in fungal virology

Suzuki, Nobuhiro

doi:10.3186/jjphytopath.83.129

Cited by 1 publication

(1 citation statement)

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“…A large collection (over 1,000) of Japanese isolates of R. necatrix was screened for viruses with potential as virocontrol agents. A series of studies showed that approximately 20% of them tested positive (Arakawa et al, 2002;Ikeda et al, 2004) and harboured diverse RNA viruses that span at least four virus families, with some remaining unassigned (Chiba et al, 2013a;Kondo et al, 2013a;Zhang et al, 2014b;Sasaki et al, 2016;Yaegashi and Kanematsu, 2016;Zhang et al, 2016;Suzuki, 2017). These studies led to the establishment of new virus families such as Megabirnaviridae and Quadriviridae (bi-or tetra-segmented dsRNA viruses) (Chiba et al, 2009;Lin et al, 2012;Lin et al, 2013;Salaipeth et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses

Arjona-López

Telengech

Jamal

et al. 2018

Environmental Microbiology

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To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is slightly lower than that (approximately 20%) previously reported for extensive surveys of over 1000 Japanese R. necatrix isolates. All viral sequences were fully or partially characterized by Sanger and next-generation sequencing. These sequences appear to represent isolates of various new species spanning at least 6 established or previously proposed families such as Partiti-, Hypo-, Megabirna-, Yado-kari-, Fusagra- and Fusarividae, as well as a newly proposed family, Megatotiviridae. This observation greatly expands the diversity of R. necatrix viruses, because no hypo-, fusagra- or megatotiviruses were previously reported from R. necatrix. The sequence analyses showed a rare horizontal gene transfer event of the 2A-like protease domain between a dsRNA (phlegivirus) and a positive-sense, single-stranded RNA virus (hypovirus). Moreover, many of the newly detected viruses showed the closest relation to viruses reported from fungi other than R. necatrix, such as Fusarium spp., which are sympatric to R. necatrix. These combined results imply horizontal virus transfer between these soil-inhabitant fungi.

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