Frontally eluted components procedure with thin layer chromatography as a mode of sample preparation for high performance liquid chromatography quantitation of acetaminophen in biological matrix

Solvent front position extraction with semi-automatic device as a powerful sample preparation procedure to quantitatitation of tryptophan in human plasma

“…As it was presented in our previous papers 30 – 32 , 35 the precision and accuracy of the SFPE technique are based on the premise that both sample components and the internal standard after chromatogram developments were evenly distributed in the focused common zone of the final solvent front position. The obtaining of the final solvent front position was preceded by additional chromatogram development which allowed cleaning the sample solution from matrix components showing weaker and stronger retention than the solutes of interest.…”

“…In our previous papers we proposed the modern approach to sample preparation procedure, especially for biological samples 30 – 32 . The procedure is named as the Solvent Front Position Extraction (SFPE) 31 and is based on the following steps 30 – 32 : (a) the internal standard (IS) is added to the sample, (b) a drop of the sample solution is applied on the chromatographic plate, (c) the chromatogram is developed to locate the substance/s of interest and internal standard/s zones at the final solvent front position, (d) the substance/s and internal standard are extracted from this zone (or its part) and transferred to the instrumental device for quantitation. The procedure was tested, with very satisfactory results, for preparation of samples of serum matrix, which was fortified with the solute of interest (acetaminophen) and internal standard (acetanilide) 30 .…”

Dispersion of multi-walled carbon nanotubes in [BMIM]PF 6 for electrochemical sensing of acetaminophen

“…As reported in the literature, the use of ACOP in excess leads to hepatotoxicity and nephrotoxicity cases [1,2]. Due to the harmful effect to human health, the development of analytical methods for its determination has increased [ [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [15]]. As a result of the ACOP's ability to oxidize electrochemically on the surface of the electrode, the number of publications related to ACOP electrochemical detection has been increased over the last years [3].…”

Desenvolvimento de método analítico empregando QuEChERS para a determinação simultânea de resíduos multiclasses de fármacos veterinários em pescados

“…Tabela 2 lista os fármacos e os valores de LMR estabelecidos para os fármacos de interesse no presente trabalho na matriz de diferentes espécies animais.Os órgãos governamentais nacionais e internacionais responsáveis pela questão de segurança alimentar, têm dedicado especial atenção ao controle e monitoramento de resíduos de medicamentos veterinários devido aos riscos à saúde, com ênfase no surgimento e desenvolvimento de resistência bacteriana.Diante disso, o monitoramento desses resíduos nos alimentos torna-se imprescindível na questão de segurança alimentar, fazendo-se necessário o desenvolvimento de métodos analíticos confiáveis que possibilitem a determinação de baixos níveis de resíduos de medicamentos de diferentes classes, objetivando a diminuição de custos, bem como a diminuição do tempo de análise, além de sua fácil adaptação para a detecção de diferentes compostos na matriz desejada, conforme a necessidade(MENDONÇA, 2012; PASCHOAL, 2007), Os resíduos de antimicrobianos podem ser monitorados através do emprego de diversas técnicas analíticas, como a cromatografia líquida ou gasosa, associada a diferentes sistemas de detecção.Devido ao fato dos alimentos constituirem-se em matrizes complexas, atenção especial deve ser dada ao preparo da amostra que antecede a análise instrumental. Esse preparo envolve um processo de extração dos analitos alvos a partir da amostra, e a subsequente purificação do extrato a fim de isolar os analitos de interesse a partir da remoção de interferentes remanescentes da matriz que poderiam afetar o desempenho da técnica analítica empregada(KLIMEK-TUREK et al, 2016;KINSELLA et al, 2009) CHOI et al, 2015;DMITRIENKO et al, 2014)…”