Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages

Lauterstein, Dana; Tijerina, Pamella B.; Corbett, K.D.; Oksuz, Betül Akgöl; Shen, Steven S.; Gordon, Terry; Klein, Catherine B.; Zelikoff, Judith T.

doi:10.3390/ijerph13040417

Cited by 77 publications

(79 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A 15‐minute break between the 2 exposure sessions was given so that the animals could access fresh air, food, and water. The puff duration was 3 seconds, puff interval 1 minute, and the puff volume was 50 mL, all of which mimics real‐life exposure scenarios 24, 25, 26, 27, 28, 29, 30, 31, 32, 33. As for the controls or control mice, they were matched in terms of age and sex (ie, 10‐week‐old males), and were exposed to clean air.…”

Section: Methodsmentioning

confidence: 99%

Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice

Qasim

Karim

Silva‐Espinoza

et al. 2018

JAHA

View full text Add to dashboard Cite

BackgroundCardiovascular disease is the main cause of death in the United States, with smoking being the primary preventable cause of premature death, and thrombosis being the main mechanism of cardiovascular mortality in smokers. Due to the perception that electronic/e‐cigarettes are “safer/less harmful” than conventional cigarettes, their usage—among a variety of ages—has increased tremendously during the past decade. Notably, there are limited studies regarding the negative effects of e‐cigarettes on the cardiovascular system, which is also the subject of significant debate.Methods and ResultsWe employed a passive e‐VapeTM vapor inhalation system and developed an in vivo whole‐body e‐cigarette mouse exposure protocol that mimics real‐life human exposure scenarios/conditions and investigated the effects of e‐cigarettes and clean air on platelet function and thrombogenesis. Our results show that platelets from e‐cigarette–exposed mice are hyperactive, with enhanced aggregation, dense and α granule secretion, activation of the αIIbβ3 integrin, phosphatidylserine expression, and Akt and ERK activation, when compared with clean air–exposed platelets. E‐cigarette–exposed platelets were also found to be resistant to inhibition by prostacyclin, relative to clean air. Furthermore, the e‐cigarette–exposed mice exhibited a shortened thrombosis occlusion and bleeding times.ConclusionsTaken together, our data demonstrate for the first time that e‐cigarettes alter physiological hemostasis and increase the risk of thrombogenic events. This is attributable, at least in part, to the hyperactive state of platelets. Thus, the negative health consequences of e‐cigarette exposure should not be underestimated and warrant further investigation.

show abstract

Section: Methodsmentioning

confidence: 99%

Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice

Qasim

Karim

Silva‐Espinoza

et al. 2018

JAHA

View full text Add to dashboard Cite

show abstract

“…ECAG is a versatile device that was designed to accommodate e-cig smoking style (Lauterstein et al 2016). The e-cig emissions are generated utilizing a “push through” air flow approach which does not require e-cig emission to go through pumps, which may introduce artifacts.…”

Section: Methodsmentioning

confidence: 99%

Development and characterization of electronic-cigarette exposure generation system (Ecig-EGS) for the physico-chemical and toxicological assessment of electronic cigarette emissions

Zhao¹,

Pyrgiotakis²,

Demokritou³

2016

Inhalation Toxicology

View full text Add to dashboard Cite

Electronic cigarettes (e-cig) have been introduced as a nicotine replacement therapy and have gained increasing attention and popularity. However, while findings on possible toxicological implications continue to grow, major knowledge gaps on both the complex chemistry of the exposure and toxicity exist, prohibiting public health assessors from assessing risks. Here a versatile electronic cigarette exposure generation system (Ecig-EGS) has been developed and characterized. Ecig-EGS allows generation of real world e-cig emission profiles under controlled operational conditions, real time monitoring and time-integrated particle/gas sampling for physico-chemical characterization, and toxicological assessment (both in vitro and in vivo). The platform is highly versatile and can be used with all e-cig types. It enables generation of precisely controlled e-cig exposure while critical operational parameters and environmental mixing conditions can be adjusted in a systematic manner to assess their impact on complex chemistry and toxicity of emissions. Results proved the versatility and reproducibility of Ecig-EGS. E-cig emission was found to contain 106 – 107 particles/cm3 with the mode diameter around 200nm, under air change rate of 60/h. Elevated CO2 and volatile organic specie generation was also observed. Furthermore, environmental mixing conditions also influenced e-cig emission profile. The versatility of Ecig-EGS will enable linking of operational and environmental parameters with exposure chemistry and toxicology and help in assessing health risks.

show abstract

“…A review of extant in vivo and in vitro studies relevant to vaping and pregnancy outcomes offers reason for concern and evidence for the necessity to pursue investigations into the effects of e‐cigarette aerosol inhalation on embryonic/fetal development. Analyses of offspring birth weight subsequent to exposure of pregnant mice to nicotine‐containing e‐vapors have failed to show reduced offspring birth weight compared to those from sham exposed and e‐vapor exposed dams (Chen, Li, Chan, et al, ; Lauterstein et al, ). Confirmation of this outcome in humans, however, is particularly important since the most well documented adverse outcome of exposure to tobacco smoke during pregnancy is infant low birth weight (Harrod et al, ).…”

Section: Are Ends a “Safer Alternative” To Tobacco Smoking During Prementioning

confidence: 99%

“…Pregnant mice exposed to nicotine‐containing e‐vapor exhibited an increase in pro‐inflammatory cytokines in the lungs of both dams and offspring (Chen, Li, Chan, Nguyen, et al, ) and altered neurodevelopment (Lauterstein et al, ; Zelikoff, Parmalee, Corbett, et al, ). Such changes were seen with both nicotine‐free and nicotine‐containing e‐vapor, suggesting that the effects were likely due to by‐products of vaporization rather than nicotine itself.…”

Section: Are Ends a “Safer Alternative” To Tobacco Smoking During Prementioning

confidence: 99%

Developmental toxicity of e‐cigarette aerosols

Greene

Pisano

2019

Birth Defects Research

View full text Add to dashboard Cite

Maternal smoking during pregnancy represents a major public health concern increasing the risk for low birth weight, congenital anomalies, preterm birth, fetal mortality, and morbidity. In an effort to diminish adverse developmental effects of exposure to cigarette smoking, pregnant women, and women of reproductive age, are increasingly turning to electronic nicotine delivery systems (ENDS), such as e‐cigarettes, as an alternative. Given that health risks associated with ENDS use during pregnancy are largely unknown, there is an acute need to determine risks vs. benefits of e‐cigarette use by pregnant women. While the most recent Surgeon General's Report on the “Health Consequences of Smoking” states that “the evidence is sufficient to infer that nicotine adversely affects maternal and fetal health during pregnancy, contributing to multiple adverse outcomes,” it remains unclear whether use of ENDS represents a “safer alternative” to tobacco smoking during pregnancy. This is due, in part, to the lack of sufficient and conclusive evidence concerning whether or not maternal e‐cigarette use adversely affects embryonic/fetal development. While several recent developmental studies have challenged the safety of nicotine inhalation via ENDS, the true risks of smoking e‐cigarettes during the first trimester of pregnancy—the period of organogenesis—are largely unknown. Moreover, evidence is emerging that even nicotine‐free e‐cigarette aerosols may harm the developing conceptus, suggesting that components of e‐cigarette liquid, including flavorings, may be developmentally toxicity. Focused human epidemiological analyses, and carefully designed animal studies are critically needed to address the question of the safety of ENDS use during pregnancy.

show abstract

Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages

Cited by 77 publications

References 41 publications

Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice

Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice

Development and characterization of electronic-cigarette exposure generation system (Ecig-EGS) for the physico-chemical and toxicological assessment of electronic cigarette emissions

Developmental toxicity of e‐cigarette aerosols

Contact Info

Product

Resources

About