Front Microrheology of Biological Fluids

Trejo-Soto, Claudia; Costa-Miracle, E.; Rodríguez-Villarreal, A. I.; Cid, Joan; Castro, Mario; Alarcón, Tomás; Hernández–Machado, A.

doi:10.1088/1742-6596/1043/1/012058

Cited by 3 publications

(3 citation statements)

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“…Control anticoagulated blood plasma had negligible shear elasticity G and its mean shear viscosity μ was ∼1.5 cP, as previously reported 48,49 (Fig. 7A and B, circles).…”

Section: Resultssupporting

confidence: 85%

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Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy

2022

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“…Control anticoagulated blood plasma had negligible shear elasticity G and its mean shear viscosity μ was ∼1.5 cP, as previously reported 48,49 (Fig. 7A and B, circles).…”

Section: Resultssupporting

confidence: 85%

“…7C). Its viscosity increased This journal is © The Royal Society of Chemistry 2022 from about 5.4 cP before the onset of coagulation 49,50 to 20.7 cP at 15 min of coagulation (Fig. 7D).…”

Section: Resultsmentioning

confidence: 99%

Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy

2022

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“…In this work, a PoC front-microrheometer with electronic detection is used to characterize blood samples from different donors. The viscosity value as a function of the shear rate of the blood front is measured, details on the mathematical model developed can be found in previous works ( Trejo-Soto et al, 2017 , Trejo-Soto et al, 2018 ; Méndez-Mora et al, 2021 ). Three types of samples are analyzed: control samples from healthy donors, and blood samples from IDA and β-TT patients.…”

Section: Introductionmentioning

confidence: 99%

Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

et al. 2021

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The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of β-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, β-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA).

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Altered blood rheology in multiwidth microchannels: Hematocrit and tonicity variation

Riera-Llobet,

Méndez-Mora,

Cabello-Fusarés

et al. 2023

Physics of Fluids

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We present a theoretical model for the characterization of the behavior of Newtonian and non-Newtonian fluids inside a microchannel with segments of different widths. This allows us to find the relation required to have collapsed viscosity curves for different fluids in just one experiment. Diverse experiments have been carried out with our setup. We consider different hematocrit concentration samples as well as samples of blood altered with different solutions in order to modify their tonicity. The data are acquired using microscale electronic detection of a fluid/air front advance. This will disclose different diseases that can be distinguished by the change in the normal rheological characteristics of blood or plasma. The results obtained demonstrate that using our setup and mathematical model, we can both distinguish blood with different concentrations of erythrocytes as well as discriminate when a same sample of blood is non-altered, altered with sodium chloride (NaCl) or de-ionized water (DIW), which emulates the abnormalities in the red blood cells of some pathologies. The results show that DIW causes blood to have more non-Newtonian behavior. On the other hand, NaCl causes the sample to become a hypertonic solution, causing the red blood cells to crenate, causing the blood to have a more Newtonian behavior.

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Front Microrheology of Biological Fluids

Cited by 3 publications

References 0 publications

Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy

Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy

Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

Altered blood rheology in multiwidth microchannels: Hematocrit and tonicity variation

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