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Altman, Russ B.; Dunker, A. Keith; Hunter, Lawrence; Ritchie, Marylyn D.; Murray, Tiffany; Klein, Teri E.

doi:10.1142/9789814749411_fmatter

Cited by 2 publications

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“…Our working hypothesis predicted that exploring the relationship between the morphology and transcriptomic profile of a neuron population might reveal clues to how neurons grow, adapt, and function (de la Torre‐Ubieta & Bonni, 2011 ; Gutierrez & Davies, 2011 ). However, since neurons are highly intertwined, postmitotic cells, they do not react well to the physical strains accompanying standard dissociation protocols (Altman et al , 2016 ). This limitation worsens as the brain matures and the properties of the extracellular matrix change, limiting most studies to the isolation of nuclei from the mature brain (Habib et al , 2016 ), which do not retain a fluorescent signal for FACS enrichment.…”

Section: Introductionmentioning

confidence: 99%

High‐throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory‐deprived cortical cholinergic VIP/CHAT neurons

et al. 2022

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Cortical neuronal networks control cognitive output, but their composition and modulation remain elusive. Here, we studied the morphological and transcriptional diversity of cortical cholinergic VIP/ChAT interneurons (VChIs), a sparse population with a largely unknown function. We focused on VChIs from the whole barrel cortex and developed a high-throughput automated reconstruction framework, termed PopRec, to characterize hundreds of VChIs from each mouse in an unbiased manner, while preserving 3D cortical coordinates in multiple cleared mouse brains, accumulating thousands of cells. We identified two fundamentally distinct morphological types of VChIs, bipolar and multipolar that differ in their cortical distribution and general morphological features. Following mild unilateral whisker deprivation on postnatal day seven, we found after three weeks both ipsi-and contralateral dendritic arborization differences and modified cortical depth and distribution patterns in the barrel fields alone. To seek the transcriptomic drivers, we developed NuNeX, a method for isolating nuclei from fixed tissues, to explore sorted VChIs. This highlighted differentially expressed neuronal structural transcripts, altered exitatory innervation pathways and established Elmo1 as a key regulator of morphology following deprivation.

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Section: Introductionmentioning

confidence: 99%

High‐throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory‐deprived cortical cholinergic VIP/CHAT neurons

et al. 2022

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“…Any practical implementation of the concept of the “exposome” [ 6 , 7 ] (the totality of exposures from prenatal to death) necessitates broad changes in the manner in which scientific research is conducted and shared [ 8 ]. While the idea of quantifying the exposome for every possible exposure may be impractical to nearly impossible in many cases with today’s state of the science, the use of sensor technologies for personal exposure monitoring may allow us, in the near future, to come close to quantifying an exposome for a limited number of chemical stressors.…”

Section: Introductionmentioning

confidence: 99%

Exposure Science in the 21st Century: Advancing the Science and Technology of Environmental Sensors through Cooperation and Collaboration across U.S. Federal Agencies

et al. 2020

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The convergence of technological innovations in areas such as microelectronics, fabrication, the Internet-of-things (IoT), and smartphones, along with their associated “apps”, permeates many aspects of life. To that list we now can add environmental monitoring. Once the sole purview of governments and academics in research, this sector is currently experiencing a transformation that is democratizing monitoring with inexpensive, portable commodities available through online retailers. However, as with any emerging area, several challenges and infrastructural hurdles must be addressed before this technology can be fully adopted and its potential be realized. A unique aspect of environmental sensing that differentiates it from some other technology sectors is its strong intersection and overlap with governance, public policy, public health, and national security—all of which contain some element of inherent governmental function. This paper advocates for and addresses the role of sensors in exposure science and illustrates areas in which improved coordination and leveraging of investments by government have helped and would catalyze further development of this technology sector.

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Front Matter

Cited by 2 publications

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High‐throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory‐deprived cortical cholinergic VIP/CHAT neurons

High‐throughput morphometric and transcriptomic profiling uncovers composition of naïve and sensory‐deprived cortical cholinergic VIP/CHAT neurons

Exposure Science in the 21st Century: Advancing the Science and Technology of Environmental Sensors through Cooperation and Collaboration across U.S. Federal Agencies

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