Front-face fluorometry of liquid samples

Eisinger, J.; Flores, Jorge

doi:10.1016/0003-2697(79)90783-8

Cited by 117 publications

(49 citation statements)

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“…high concentrations of the fluorescent species. To avoid these problems, the method of front-face fluorescence spectroscopy (Figure 1) can be used for bulk liquid samples and solid samples (Parker 1968;Hirschfeld 1978;Eisinger & Flores 1979;Blumberg et al 1980;Hirsch & Nagel 1989;Genot et al 1992a, b;Sóti et al 1993;Herbert et al 2000;Patra & Mishra 2002). The incidence angle of the excitation radiation is usually set at 56° (Table 1).…”

Section: Fluorescence Spectroscopy and Chemometrics In The Food Classmentioning

confidence: 99%

Fluorescence spectroscopy and chemometrics in the food classification - a review

Sádecká¹,

Tóthová²

2007

Czech J. Food Sci.

181

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Sádecká J., Tóthová J. (2007): Fluorescence spectroscopy and chemometrics in the food classification − a review. Czech J. Food Sci., 25: 159-173.This review deals with the last few years' articles on various fluorescence techniques (conventional, excitation-emission matrix, and synchronous fluorescence spectroscopy) as a tool for the classification of food samples. Chemometric methods as principal component analysis, hierarchical cluster analysis, parallel factor analysis, and factorial discriminate analysis are briefly reminded. The respective publications are then listed according to the food samples: dairy products, eggs, meat, fish, edible oils, and others.

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Section: Fluorescence Spectroscopy and Chemometrics In The Food Classmentioning

confidence: 99%

Fluorescence spectroscopy and chemometrics in the food classification - a review

Sádecká¹,

Tóthová²

2007

Czech J. Food Sci.

181

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“…In front face illumination, the fluorescence intensities become independent of concentration at high optical densities and all the incident light is absorbed near the surface of the cuvette. Moreover, in front-face geometry the exciting and emitted light enter and leave the same cell face 50 . The excitation light penetrates a very short distance of the sample.…”

Section: Preparation Of Asphaltene -Prodan Solutionsmentioning

confidence: 99%

Changes in Asphaltene Microenvironments Evidenced by Fluorescence Solvatochromism

Pietraru

Cramb

2003

Langmuir

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This work involved the study of Cold Lake asphaltenes' behavior in toluene and o-dichlorobenzene. The changes in asphaltene microenvironments with the increase in asphaltene concentration were observed using fluorescence solvatochromism. The reporter molecule chosen was the environmentally sensitive fluorophore PRODAN. This procedure represents a novel and sensitive method to study asphaltene solutions, and might bring additional information to the existent database of asphaltene properties.A pre-aggregation model was developed to explain the experimental observations, and a definition for the critical aggregation concentration was proposed. The model is valid for low asphaltene concentrations and is supported by direct measurements of refractive indices for asphaltene solutions. Atomic force microscopy was used to image asphaltene entities. The size distribution indicated that asphaltene particles do not change significantly with the increase in concentration. The appearance of larger aggregates in solutions with concentrations above CAC might support the validity of the model.

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“…These were indistinguishable from the comparable absorption spectra of Hb A. The fluorescence excitation and emission maxima and intensity of Hb Evanston were also the same as those of Hb A when they were determined by a front surface method (27) or with short path length cuvettes.…”

Section: Resultsmentioning

confidence: 63%

“…Fluorometric studies of the purified hemoglobins were performed with short path-length cuvettes and by a front surface technique (27). For these determinations the hemoglobins were prepared in Tris-glycine buffer, pH 7.0, at a concentration of 90 uM.…”

Section: Methodsmentioning

confidence: 99%

Hemoglobin Evanston (alpha 14 Trp----Arg). An unstable alpha-chain variant expressed as alpha-thalassemia.

Honig¹,

Shamsuddin²,

Vida³

et al. 1984

J. Clin. Invest.

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Atypctract. A new hematologic syndrome with phenotypic features of mild Hb H disease was identified in three children from two unrelated black American families. Erythrocytes from each of these children contained Hb H (04) and Hb Barts (74), as well as a slowly migrating hemoglobin fraction that made up 7-10% of the total hemoglobin. The parents ofthe affected children all showed mild thalassemia-like changes, with one ofthe parents in each family also expressing the variant hemoglobin; in the latter individuals the mutant a-chains made up <2% of the total, and were present mainly or exclusively in combination with 5-chains in the form of a slowly migrating Hb A2. Purified Hb Evanston showed an increased oxygen affinity, but its Bohr effect, cooperativity, and 2,3-diphosphoglycerate effect were normal. to heat and to isopropanol, and the stability of its a-chain in an extended time course synthesis study also appeared to be similar to that of aA. However, the results from short-term globin synthesis studies, and from mRNA translation in vitro, suggest that the two types of a-chains were synthesized at relatively equal rates, with a major fraction of the newly synthesized variant a-chains undergoing rapid catabolism. The hematologic data taken in combination with DNA hybridization and globin synthesis findings indicate that the proposita in each of these families has the genotype -,aAl/,aEv. These observations suggest that two separate mechanisms are contributing to the a-thalassemia-like expression of Hb Evanston: (a) the newly synthesized aEV-chains are unstable and are subject to early proteolytic destruction; and (b) the mutant a-allele is linked to an a-globin gene deletion.

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Front-face fluorometry of liquid samples

Cited by 117 publications

References 1 publication

Fluorescence spectroscopy and chemometrics in the food classification - a review

Fluorescence spectroscopy and chemometrics in the food classification - a review

Changes in Asphaltene Microenvironments Evidenced by Fluorescence Solvatochromism

Hemoglobin Evanston (alpha 14 Trp----Arg). An unstable alpha-chain variant expressed as alpha-thalassemia.

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