Front Cover: Solution‐Phase Synthesis of Branched Oligonucleotides with up to 32 Nucleotides and the Reversible Formation of Materials (Eur. J. Org. Chem. 39/2017)

Abstract

The Front Cover shows the starting materials of a solution‐phase synthesis of branched oligonucleotides and a series of reaction vessels of increasing size, illustrating the fact that there is a new, efficient, and scalable DNA synthesis that provides macroscopic amounts of branched DNA. While traditional solid‐phase synthesis of DNA is expensive to scale up, the new method readily produces hundreds of milligrams of branched oligonucleotides in a cost‐efficient manner using conventional laboratory equipment. This is important for producing DNA‐based materials in macroscopic quantities. More information can be found in the https://doi.org/10.1002/ejoc.201700686

Liquid‐Phase Oligonucleotide Synthesis: Past, Present, and Future Predictions

“…The synthesis of ONs on a tetra-branched adamantane soluble support has recently been reported by Richert and co-workers (Schwenger et al, 2017; Fig. 7).…”

“…Over the last decade, numerous LPOS strategies have been explored in numerous research laboratories (de Koning et al, 2006;Donga, Khaliq-Uz-Zaman, Chan, & Damha, 2006;Jabgunde et al, 2015;Kim, Matsumoto, & Chiba, 2013;Kungurtsev, Laakkonen, Molina, & Virta, 2013;Matsuno, Shoji, Kim, & Chiba, 2016;Molina, Jabgunde, Virta, & Lönnberg, 2014;Molina, Kungurtsev, Virta, & Lönnberg, 2012;Schwenger, Birchall, & Richert, 2017). Like PEG-based soluble supports, these carriers facilitate a fast and high yielding ON synthesis.…”

“…Adamantane-core (Schwenger et al, 2017) 8-mer (32 nucleotides, 4-arm E (×1) + P (×4) 2 eq./branch (DNA)…”

“…This methodology was employed for the assembly of ONs in a facile manner. Although the amidite protocol facilitated the synthesis Overview of Richert's LPOS strategy using a tetrameric adamantane-derived core (Schwenger et al, 2017).…”

“…During the last decade, various chemical protocols for LPOS have been a subject of significant interest due to the increasing demand for large quantities of therapeutic ONs. This effort includes extraction (de Koning et al, 2006), precipitation (Donga et al, 2006;Kim et al, 2013;Kungurtsev et al, 2013;Matsuno et al, 2016;Molina et al, 2015;Molina et al, 2014;Schwenger et al, 2017), chromatography (Molina et al, 2012), and OSNbased (Gaffney et al, 2015;Kim et al, 2016) purification techniques for scale up of ONs. Although some of these methods provide possible avenues for large-scale LPOS, none have been demonstrated at scales useful for the commercial production of ONs.…”