From wandering weeds to pharmacy: An insight into traditional uses, phytochemicals and pharmacology of genus Chromolaena (Asteraceae)

Ojo, Olusesan; Mphahlele, Mokgadi Precious; Oladeji, Olatunde S.; Mmutlane, Edwin M.; Ndinteh, Derek Tantoh

doi:10.1016/j.jep.2022.115155

Cited by 12 publications

(14 citation statements)

References 82 publications

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“…Approximately 79 flavonoids, comprising 9 chalcones, have been isolated and identified from the genus Chromolaena , as noted in reference [ 23 ]. This research culminated in the isolation and identification of a specific chalcone derived from the inflorescences of Chromolaena tacotana .…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally in ethnomedicine, Chromolaena species have been employed to treat a variety of ailments, including malaria, nasal congestion, inflammation, eye disorders, asthma, cough, flu, headache, colds, and, more recently, cancer [ 21 , 22 ]. Over 190 compounds have been isolated and identified from 27 species within this genus, encompassing a diverse array of chemical classes, such as flavonoids, alkaloids, triterpenoids, diterpenoids, sesquiterpenoids, steroids, fatty acids, coumarins, and chalcones [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Approximately nine chalcones have been isolated from the Chromolaena genus. These include pinocembrin chalcone (2 ′ ,4 ′ ,6 ′ -Trihydroxychalcone) from Chromolaena chasea and several compounds from Chromolaena odorata: 2 ′ ,4-dihydroxy-4 ′ ,5 ′ ,6 ′ -trimethoxy chalcone (ChDora), 2 ′ -hydroxy-3 ′ ,4,4 ′ ,5 ′ ,6 ′ -pentamethoxychalcone (Odoratin), 2 ′ -hydroxy-4,4 ′ ,5 ′ ,6 ′tetramethoxychalcone, 4,6 ′ -dihydroxy-2 ′ ,3 ′ ,4 ′ -trimethoxychalcone, 6 ′ -hydroxy-4,2 ′ ,3 ′ ,4 ′tetramethoxychalcone, 2 ′ ,4-dihydroxy-3 ′ ,4 ′ ,6 ′ -trimethoxychalcone, 2 ′ -hydroxy-3 ′ ,4,4 ′ ,6 ′tetramethoxychalcone, and 2-hydroxy-4, ′ 5, ′ 6, ′ 4,5-pentamethoxychalcone [23][24][25]. Odoratin has been observed to decrease cell viability in Cal51, HeLa, and MCF-7 breast cancer cells in a dose-dependent manner [23].…”

Section: Introductionmentioning

confidence: 99%

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Natural 2′,4-Dihydroxy-4′,6′-dimethoxy Chalcone Isolated from Chromolaena tacotana Inhibits Breast Cancer Cell Growth through Autophagy and Mitochondrial Apoptosis

Mendez-Callejas,

Piñeros-Avila,

Celis

et al. 2024

Plants

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Breast cancer (BC) is one of the most common cancers among women. Effective treatment requires precise tailoring to the genetic makeup of the cancer for improved efficacy. Numerous research studies have concentrated on natural compounds and their anti-breast cancer properties to improve the existing treatment options. Chromolaena tacotana (Klatt) R.M. King and H. Rob (Ch. tacotana) is a notable source of bioactive hydroxy-methylated flavonoids. However, the specific anti-BC mechanisms of these flavonoids, particularly those present in the plant’s inflorescences, remain partly undefined. This study focuses on assessing a chalcone derivative extracted from Ch. tacotana inflorescences for its potential to concurrently activate regulated autophagy and intrinsic apoptosis in luminal A and triple-negative BC cells. We determined the chemical composition of the chalcone using ultraviolet (UV) and nuclear magnetic resonance (NMR) spectroscopy. Its selective cytotoxicity against BC cell lines was assessed using the MTT assay. Flow cytometry and Western blot analysis were employed to examine the modulation of proteins governing autophagy and the intrinsic apoptosis pathway. Additionally, in silico simulations were conducted to predict interactions between chalcone and various anti-apoptotic proteins, including the mTOR protein. Chalcone was identified as 2′,4-dihydroxy-4′,6′-dimethoxy-chalcone (DDC). This compound demonstrated a selective inhibition of BC cell proliferation and triggered autophagy and intrinsic apoptosis. It induced cell cycle arrest in the G0/G1 phase and altered mitochondrial outer membrane potential (∆ψm). The study detected the activation of autophagic LC3-II and mitochondrial pro-apoptotic proteins in both BC cell lines. The regulation of Bcl-XL and Bcl-2 proteins varied according to the BC subtype, yet they showed promising molecular interactions with DDC. Among the examined pro-survival proteins, mTOR and Mcl-1 exhibited the most favorable binding energies and were downregulated in BC cell lines. Further research is needed to fully understand the molecular dynamics involved in the activation and interaction of autophagy and apoptosis pathways in cancer cells in response to potential anticancer agents, like the hydroxy-methylated flavonoids from Ch. tacotana.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Natural 2′,4-Dihydroxy-4′,6′-dimethoxy Chalcone Isolated from Chromolaena tacotana Inhibits Breast Cancer Cell Growth through Autophagy and Mitochondrial Apoptosis

Mendez-Callejas,

Piñeros-Avila,

Celis

et al. 2024

Plants

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“…More than 190 different phytochemicals have been isolated from C. odorata and identified, such as fatty acids, triterpenoids, diterpenes, sesquiterpenes, essential oils, alkaloids, anthracene derivatives, flavonoids, stilbenes, and derivatives of diterpene [ 26 ]. Most flavonoids and phenolic acids, such as odoratenin, persicogenin, and (Z)-p-coumaric acid, were found in the aerial parts and leaves of C. odorata [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of ethyl acetate extract of Chromolaena odorata on MCF7 and T47D breast cancer cells

Yusuf,

Novia,

Fahriani

2023

Narra J

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One of the most cancers in women that can be fatal is breast cancer. Radiation therapy, chemotherapy, or a combination of the two are often used to treat cancer, and these treatments tend to modify the immune system and weaken defences. Using natural compounds from plants has become a research interest to prevent cancer cell development. The aim of this study was to determine the anticancer activities of ethyl acetate extract of Chromolaena odorata (EACO) against breast cancer cells (MCF-7 and T47D). The viability of the cells was determined by the MTT colorimetry assays. The apoptosis test was performed by using flow cytometry. The IC50 value for MCF-7 cells was 218.78 μg/mL and 307.61 μg/mL for T47D. The extract acted selectively against breast cancer cells, with selectivity indexes against MCF-7 and T47D were 12.77 and 9.08, respectively. The viable cells of T47D cells were decreased from 85±36.5% (24 hours) to 54±34% (48 hours) after treatment with IC50 of EACO. Significant decrease of the MCF-7's viable cells were observed between 48 and 72 hours after treatment with IC50 (68.5±17.7% to 51.01±12.1%, respectively). Apoptosis assay showed that T47D and MCF-7 cells were mainly in the necrosis stage (83.35±0.49% and 95.15±1.76%, respectively). This study suggested that ethyl acetate extract of C. odorata is promising to be developed as an anticancer agent.

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“…Traditional therapy using natural products has long been used for anticancer, antioxidant, and anti-inflammatory purposes, involving flavonoid products, of which curcumin, myricetin, and quercetin are among the best known examples [ 98 ]. Curcumin was found to be an antioxidant and anticancer agent by irreversible covalent modification of the redox-active residues Cys 496 and Sec 497 in TrxR [ 99 ].…”

Section: Inhibitors and Activators Of The Trx/trxr Systemmentioning

confidence: 99%

The Thioredoxin System of Mammalian Cells and Its Modulators

Hasan

Tatarskiy

Shtil³

2022

Biomedicines

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Oxidative stress involves the increased production and accumulation of free radicals, peroxides, and other metabolites that are collectively termed reactive oxygen species (ROS), which are produced as by-products of aerobic respiration. ROS play a significant role in cell homeostasis through redox signaling and are capable of eliciting damage to macromolecules. Multiple antioxidant defense systems have evolved to prevent dangerous ROS accumulation in the body, with the glutathione and thioredoxin/thioredoxin reductase (Trx/TrxR) systems being the most important. The Trx/TrxR system has been used as a target to treat cancer through the thiol–disulfide exchange reaction mechanism that results in the reduction of a wide range of target proteins and the generation of oxidized Trx. The TrxR maintains reduced Trx levels using NADPH as a co-substrate; therefore, the system efficiently maintains cell homeostasis. Being a master regulator of oxidation–reduction processes, the Trx-dependent system is associated with cell proliferation and survival. Herein, we review the structure and catalytic properties of the Trx/TrxR system, its role in cellular signaling in connection with other redox systems, and the factors that modulate the Trx system.

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From wandering weeds to pharmacy: An insight into traditional uses, phytochemicals and pharmacology of genus Chromolaena (Asteraceae)

Cited by 12 publications

References 82 publications

Natural 2′,4-Dihydroxy-4′,6′-dimethoxy Chalcone Isolated from Chromolaena tacotana Inhibits Breast Cancer Cell Growth through Autophagy and Mitochondrial Apoptosis

Natural 2′,4-Dihydroxy-4′,6′-dimethoxy Chalcone Isolated from Chromolaena tacotana Inhibits Breast Cancer Cell Growth through Autophagy and Mitochondrial Apoptosis

Cytotoxic activity of ethyl acetate extract of Chromolaena odorata on MCF7 and T47D breast cancer cells

The Thioredoxin System of Mammalian Cells and Its Modulators

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