From Transgenesis to Genome Editing in Crop Improvement: Applications, Marketing, and Legal Issues

Marone, Daniela; Mastrangelo, Anna Maria; Borrelli, Grazia Maria

doi:10.3390/ijms24087122

Cited by 10 publications

(14 citation statements)

References 126 publications

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“…Unlike the conventional breeding method, CRISPR-edited gene is time efficient and stable, as there is no distinguishable difference between the variants generated from genome editing and those obtained from naturally occurring variations ( Marone, Mastrangelo & Borrelli, 2023 ; Anders, Hoengenaert & Boerjan, 2023 ). It was also more readily accepted in the market for commerce ( Brandt & Barrangou, 2019 ).…”

Section: Impact Of Crispr-cas In Managing the Environmental Stress On...mentioning

confidence: 99%

Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

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Background Global food systems in recent years have been impacted by some harsh environmental challenges and excessive anthropogenic activities. The increasing levels of both biotic and abiotic stressors have led to a decline in food production, safety, and quality. This has also contributed to a low crop production rate and difficulty in meeting the requirements of the ever-growing population. Several biotic stresses have developed above natural resistance in crops coupled with alarming contamination rates. In particular, the multiple antibiotic resistance in bacteria and some other plant pathogens has been a hot topic over recent years since the food system is often exposed to contamination at each of the farm-to-fork stages. Therefore, a system that prioritizes the safety, quality, and availability of foods is needed to meet the health and dietary preferences of everyone at every time. Methods This review collected scattered information on food systems and proposes methods for plant disease management. Multiple databases were searched for relevant specialized literature in the field. Particular attention was placed on the genetic methods with special interest in the potentials of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and Cas (CRISPR associated) proteins technology in food systems and security. Results The review reveals the approaches that have been developed to salvage the problem of food insecurity in an attempt to achieve sustainable agriculture. On crop plants, some systems tend towards either enhancing the systemic resistance or engineering resistant varieties against known pathogens. The CRISPR-Cas technology has become a popular tool for engineering desired genes in living organisms. This review discusses its impact and why it should be considered in the sustainable management, availability, and quality of food systems. Some important roles of CRISPR-Cas have been established concerning conventional and earlier genome editing methods for simultaneous modification of different agronomic traits in crops. Conclusion Despite the controversies over the safety of the CRISPR-Cas system, its importance has been evident in the engineering of disease- and drought-resistant crop varieties, the improvement of crop yield, and enhancement of food quality.

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Section: Impact Of Crispr-cas In Managing the Environmental Stress On...mentioning

confidence: 99%

Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

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“…The utilization of biotechnology is of paramount importance in managing insect pests to safeguard crops and enhance yields. This encompasses a broad spectrum of activities, ranging from the development of pest-resistant breeds to the genetic modification of novel genes through introgression [41]. The developing stage of utilizing genome-editing methodologies for insect-resistant plants is currently underway.…”

Section: Potentiality Of Revolutionizing Pest Management Using Crispr...mentioning

confidence: 99%

Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management

Komal,

Desai,

Samal

et al. 2023

Plants

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Phytophagous insects pose a significant threat to global crop yield and food security. The need for increased agricultural output while reducing dependence on harmful synthetic insecticides necessitates the implementation of innovative methods. The utilization of CRISPR-Cas (Clustered regularly interspaced short palindromic repeats) technology to develop insect pest-resistant plants is believed to be a highly effective approach in reducing production expenses and enhancing the profitability of farms. Insect genome research provides vital insights into gene functions, allowing for a better knowledge of insect biology, adaptability, and the development of targeted pest management and disease prevention measures. The CRISPR-Cas gene editing technique has the capability to modify the DNA of insects, either to trigger a gene drive or to overcome their resistance to specific insecticides. The advancements in CRISPR technology and its various applications have shown potential in developing insect-resistant varieties of plants and other strategies for effective pest management through a sustainable approach. This could have significant consequences for ensuring food security. This approach involves using genome editing to create modified insects or crop plants. The article critically analyzed and discussed the potential and challenges associated with exploring and utilizing CRISPR-Cas technology for reducing insect pest pressure in crop plants.

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“…Several reports have shown that transformation efficiency in melon is notably low [ 14 , 15 ]. This limitation hinders the application of transgenesis and genome editing for functional genomics studies [ 16 , 17 ]. Different factors affect the Agrobacterium -mediated transformation efficiency, particularly those related to the inoculation and co-cultivation medium conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Melon organogenesis and embryogenesis are typically performed using leaf and cotyledon explants. However, several studies have utilized cotyledonary explants for direct shoot organogenesis [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Regeneration of melon plants from cotyledonary explants is more efficient than leaf explants, but it leads to a higher polyploidy level in the regenerated plants [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (Cucumis melo L.)

Parsa

Sabet

Moieni

et al. 2023

IJMS

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Melon is a recalcitrant plant for stable genetic transformation. Various protocols have been tried to improve melon transformation efficiency; however, it remains significantly low compared to other plants such as tomato. In this study, the primary focus was on the optimization of key parameters during the inoculation and co-culture steps of the genetic transformation protocol. Our results showed that immersing the explants in the inoculation medium for 20 min significantly enhanced transformation efficiency. During the co-culture step, the use of filer paper, 10 mM 2-(N-morpholino)-ethanesulfonic acid (MES), and a temperature of 24 °C significantly enhanced the melon transformation efficiency. Furthermore, the impact of different ethylene inhibitors and absorbers on the transformation efficiency of various melon varieties was explored. Our findings revealed that the use of these compounds led to a significant improvement in the transformation efficiency of the tested melon varieties. Subsequently, using our improved protocol and reporter-gene construct, diploid transgenic melons successfully generated. The efficiency of plant genetic transformation ranged from 3.73 to 4.83%. Expanding the scope of our investigation, the optimized protocol was applied to generate stable gene-edited melon lines using the Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9)-mediated cytosine base editor and obtained melon lines with editions (C-to-T and C-to-G) in the eukaryotic translation initiation factor 4E, CmeIF4E gene. In conclusion, the optimized melon transformation protocol, along with the utilization of the CRISPR/Cas9-mediated cytosine base editor, provides a reliable framework for functional gene engineering in melon. These advancements hold significant promise for furthering genetic research and facilitating crop improvement in this economically important plant species.

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From Transgenesis to Genome Editing in Crop Improvement: Applications, Marketing, and Legal Issues

Cited by 10 publications

References 126 publications

Adoption of CRISPR-Cas for crop production: present status and future prospects

Adoption of CRISPR-Cas for crop production: present status and future prospects

Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management

CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (Cucumis melo L.)

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