From the forest to the plate – Hemicelluloses, galactoglucomannan, glucuronoxylan, and phenolic-rich extracts from unconventional sources as functional food ingredients

Granato, Daniel; Reshamwala, Dhanik; Korpinen, Risto; Azevedo, Luciana; Carmo, Mariana Araújo Vieira do; Cruz, Thiago Mendanha; Marques, Mariza Boscacci; Wen, Mingchun; Zhang, Liang; Marjomäki, Varpu; Kilpeläinen, Petri

doi:10.1016/j.foodchem.2022.132284

Cited by 26 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent study glucuronoxylan-rich extract was shown not to be toxic to normal human cells, which supports our findings. 88 Furthermore, we did not notice a statistically significant difference between the treated and control group in food and water intake, which indicates that GX does not interfere with these parameters or reduce palatability. Body weight of the rats eating the GXpoly or pureGX diet did not differ from the control group throughout the study, suggesting that GXpoly or pureGX does not compromise nutrient absorption.…”

Section: Food and Function Papermentioning

confidence: 63%

Gut microbiota can utilize prebiotic birch glucuronoxylan in production of short-chain fatty acids in rats

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Food and Function Papermentioning

confidence: 63%

Gut microbiota can utilize prebiotic birch glucuronoxylan in production of short-chain fatty acids in rats

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The high xylose content in the mixture of the aspen HC monosaccharides indicates that glucuronoxylan is the leading polysaccharide in the obtained HCs, which is typical of hardwood HCs [ 36 , 37 ]. The presence of mannose, galactose, and glucose is indicative of glucomannan, arabinogalactan, and other polysaccharides in small amounts [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

et al. 2022

View full text Add to dashboard Cite

In this study, hemicelluloses of aspen wood (Pópulus trémula) were obtained by oxidative delignification in an acetic acid‒water‒hydrogen peroxide medium at temperatures of 70‒100 °C and a process time of 1‒4 h. The maximum polysaccharide yield of up to 9.68 wt% was reported. The composition and structure of the hemicelluloses were studied using a complex of physicochemical methods: gas and gel permeation chromatography, Fourier-transform infrared spectroscopy, 2D nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. The xylose, mannose, galactose, and glucose monomer units were identified in the hemicelluloses by gas chromatography. The weight average molecular weight Mw of the products determined by gel permeation chromatography was found to range within 8932‒33,142 g/mol. The reported Fourier-transform spectra of the hemicelluloses contain all the bands characteristic of heteropolysaccharides; a weak lignin absorption signal in the spectra at 1500‒1510 cm−1 is attributed to a minor content of phenolic fragments in the structure of the obtained hemicelluloses. The use of thermogravimetric analysis established that the hemicelluloses isolated from aspen wood are resistant against heating to temperatures of up to 90–100 °C and, upon further heating up to 400 °C, start destructing at an increasing rate. The antioxidant activity of the hemicelluloses was examined using the compounds that mimic free radicals (1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radicals (salicylic acid). It was found that the activity of all polysaccharides in neutralizing DPPH and hydroxyl radicals is lower than the absorption capacity of vitamin C at all the tested concentrations (0.5, 2, and 5 mg/mL) and attains 81.7 and 82.9%, respectively.

show abstract

“…First, the compounds were diluted with methanol at a molar concentration of 0.5 mmol/L to compare the results on a molar basis. The methods followed the experimental conditions adopted and validated in our previous studies [ 64 , 65 ]. For the DPPH assay, a 0.10 mmol/L methanolic solution of DPPH was prepared, and the absorbance decay was monitored at 517 nm after 30 min reaction.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Antioxidant Capacity and Network Pharmacology of Phloretin and Phlorizin against Neuroinflammation in Traumatic Brain Injury

2023

Self Cite

View full text Add to dashboard Cite

Neuroinflammation is a hallmark of traumatic brain injury (TBI)’s acute and chronic phases. Despite the medical and scientific advances in recent years, there is still no effective treatment that mitigates the oxidative and inflammatory damage that affects neurons and glial cells. Therefore, searching for compounds with a broader spectrum of action that can regulate various inflammatory signaling pathways is of clinical interest. In this study, we determined not only the in vitro antioxidant capacity of apple pomace phenolics, namely, phlorizin and its metabolite, phloretin, but we also hypothesize that the use of these bioactive molecules may have potential use in TBI. We explored the antioxidant effects of both compounds in vitro (DPPH, iron-reducing capacity (IRC), and Folin–Ciocalteu reducing capacity (FCRC)), and using network pharmacology, we investigated the proteins involved in their protective effects in TBI. Our results showed that the antioxidant properties of phloretin were superior to those of phlorizin in the DPPH (12.95 vs. 3.52 mg ascorbic acid equivalent (AAE)/L), FCRC (86.73 vs. 73.69 mg gallic acid equivalent (GAE)/L), and iron-reducing capacity (1.15 vs. 0.88 mg GAE/L) assays. Next, we examined the molecular signature of both compounds and found 11 proteins in common to be regulated by them and involved in TBI. Meta-analysis and GO functional enrichment demonstrated their implication in matrix metalloproteinases, p53 signaling, and cell secretion/transport. Using MCODE and Pearson’s correlation analysis, a subcluster was generated. We identified ESR1 (estrogen receptor alpha) as a critical cellular hub being regulated by both compounds and with potential therapeutic use in TBI. In conclusion, our study suggests that because of their vast antioxidant effects, probably acting on estrogen receptors, phloretin and phlorizin may be repurposed for TBI treatment due to their ease of obtaining and low cost.

show abstract

From the forest to the plate – Hemicelluloses, galactoglucomannan, glucuronoxylan, and phenolic-rich extracts from unconventional sources as functional food ingredients

Cited by 26 publications

References 46 publications

Gut microbiota can utilize prebiotic birch glucuronoxylan in production of short-chain fatty acids in rats

Gut microbiota can utilize prebiotic birch glucuronoxylan in production of short-chain fatty acids in rats

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

Comparison of Antioxidant Capacity and Network Pharmacology of Phloretin and Phlorizin against Neuroinflammation in Traumatic Brain Injury

Contact Info

Product

Resources

About