From single cells to our planet—recent advances in using mass spectrometry for spatially resolved metabolomics

Petras, Daniel; Jarmusch, Alan K.; Dorrestein, Pieter C.

doi:10.1016/j.cbpa.2016.12.018

Cited by 77 publications

(50 citation statements)

References 49 publications

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“…Unlike spectroscopic techniques, mass spectrometry (MS) allows highly selective and quantitative measurements to be taken of a broad range of chemical families over a large dynamic range. With recent advantages in imaging mass spectrometry (IMS), we can obtain metabolic inventories with spatial information from nm to km scale (Petras et al, 2017). Matrix assisted laser desorption ionization (MALDI) and laser assisted electrospray ionization (LAESI) use laser shots that raster the surface of a sample to generate molecular 2D images, while desorption based DESI and nano-DESI raster continuously flowing solvent droplets across the sample (Watrous et al, 2012; Etalo et al, 2015; Sturtevant et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Based Molecular 3D-Cartography of Plant Metabolites

et al. 2017

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Plants play an essential part in global carbon fixing through photosynthesis and are the primary food and energy source for humans. Understanding them thoroughly is therefore of highest interest for humanity. Advances in DNA and RNA sequencing and in protein and metabolite analysis allow the systematic description of plant composition at the molecular level. With imaging mass spectrometry, we can now add a spatial level, typically in the micrometer-to-centimeter range, to their compositions, essential for a detailed molecular understanding. Here we present an LC-MS based approach for 3D plant imaging, which is scalable and allows the analysis of entire plants. We applied this approach in a case study to pepper and tomato plants. Together with MS/MS spectra library matching and spectral networking, this non-targeted workflow provides the highest sensitivity and selectivity for the molecular annotations and imaging of plants, laying the foundation for studies of plant metabolism and plant-environment interactions.

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Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Based Molecular 3D-Cartography of Plant Metabolites

et al. 2017

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“…and coincide with experimental goals (sample preparation, image resolution, etc.) [46]. Matrix Assisted Laser Desorption Ionization (MALDI), Desorption Electrospray Ionization (DESI), and Liquid-Microjunction-Surface Sampling Probe (LMJ-SSP) have demonstrated success for IM-MSI applications [46–51] [52]•.…”

Section: Im-msi For Spatial Characterizationmentioning

confidence: 99%

“…[46]. Matrix Assisted Laser Desorption Ionization (MALDI), Desorption Electrospray Ionization (DESI), and Liquid-Microjunction-Surface Sampling Probe (LMJ-SSP) have demonstrated success for IM-MSI applications [46–51] [52]•. After matrix application on the surface, MALDI imaging analyses are typically performed in-vacuo with relatively high spatial resolution (dictated by the size and shape of the laser); ambient ionization techniques, such as DESI and LMJ-SSP, require minimal to no sample preparation allowing samples to be analyzed in native state, but have more moderate spatial resolution [53].…”

Section: Im-msi For Spatial Characterizationmentioning

confidence: 99%

Improving the discovery of secondary metabolite natural products using ion mobility–mass spectrometry

Schrimpe‐Rutledge

Sherrod

McLean

2018

Current Opinion in Chemical Biology

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Secondary metabolite discovery requires an unbiased, comprehensive workflow to detect unknown unknowns for which little to no molecular knowledge exists. Untargeted mass spectrometry-based metabolomics is a powerful platform, particularly when coupled with ion mobility for high-throughput gas-phase separations to increase peak capacity and obtain gas-phase structural information. Ion mobility data are described by the amount of time an ion spends in the drift cell, which is directly related to an ion's collision cross section (CCS). The CCS parameter describes the size, shape, and charge of a molecule and can be used to characterize unknown metabolomic species. Here, we describe current and emerging applications of ion mobility-mass spectrometry for prioritization, discovery and structure elucidation, and spatial/temporal characterization.

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“…Nevertheless, it is becoming an accurate and reliable protein analysis method since sequence information can be obtained even from trace amounts of target proteins. Recently, mass spectrometry imaging (also known as imaging mass spectrometry) has been studied as a way of detecting and analyzing proteins directly in specific locations in cells or tissues by imaging mass profiles (or maps) on 2D samples [67]. This technique is very similar to finding a neurotransmitter in a specific section of the brain using magnetic resonance imaging.…”

Section: Purification and Analysismentioning

confidence: 99%

A Simple Outline of Methods for Protein Isolation and Purification

Lee

2017

Endocrinol Metab

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At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.

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From single cells to our planet—recent advances in using mass spectrometry for spatially resolved metabolomics

Cited by 77 publications

References 49 publications

Mass Spectrometry Based Molecular 3D-Cartography of Plant Metabolites

Mass Spectrometry Based Molecular 3D-Cartography of Plant Metabolites

Improving the discovery of secondary metabolite natural products using ion mobility–mass spectrometry

A Simple Outline of Methods for Protein Isolation and Purification

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