From seed germination to flowering, light controls plant development via the pigment phytochrome.

Chory, Joanne; Chatterjee, Mithu; Cook, Ron; Elich, Tedd D.; Fankhauser, Christian; Li, J.; Nagpal, Punita; Neff, Michael M.; Pepper, Alan E.; Poole, Daniel S.; Reed, Jason W.; Vitart, Véronique

doi:10.1073/pnas.93.22.12066

Cited by 191 publications

(162 citation statements)

References 65 publications

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“…Thus, a fine tailoring of the illumination spectrum, which is feasible using the LED technology, is of commercial importance for growing Chrysanthemum plantlets in vitro. According to Chory et al [21], many processes of plant development are controlled by light via the systems of phytochromes and phytohormones. Therefore, a further study of interaction between the illumination spectrum and endogenous growth regulators on plantlets rooting would be interesting.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of Chrysanthemum plantlets using light-emitting diodes

Kurilčik¹,

Miklušytė-Čanova

Dapkūnienė

et al. 2008

Open Life Sciences

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Effects of illumination spectrum on the morphogenesis of chrysanthemum plantlets (Chrysanthemum morifolium Ramat. 'Ellen') grown in vitro were studied using an illumination system consisting of four groups of light-emitting diodes (LEDs) in the following spectral regions: blue (450nm), red (640nm), red (660nm), and far-red (735nm). Taking into account all differences in shoot height, root length, and fresh and dry weight (FW and DW, respectively), observed while changing the total photon flux density (PFD), the optimal total PFD for growth of chrysanthemum plantlets in vitro was estimated. For 16 h photoperiod and typical fractions of the spectral components (14%, 50%, 28%, and 8%, respectively), the optimal total PFD was found to be 40 µmol m -2 s -1. Our study shows that the blue component in the illumination spectrum inhibits the plantlet extension and formation of roots and simultaneously increases the DW to FW ratio and content of photosynthetic pigments. We demonstrate photomorphogenetic effects in the blue region and its interaction with the fractional PFD of the far-red spectral component. Under constant fractional PFD of the blue component, the root number, length of roots and stems, and fresh weight of the plantlets have a correlated nonmonotonous dependence on the fractional PFD of the far-red component.

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Section: Discussionmentioning

confidence: 99%

In vitro culture of Chrysanthemum plantlets using light-emitting diodes

Kurilčik¹,

Miklušytė-Čanova

Dapkūnienė

et al. 2008

Open Life Sciences

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“…Proteins affecting processes that can be regulated by phytochrome have been identified by the mutation of genes (designated DETlOLATED [DET]/CONSTITUT/V€ PHOTOMORPHOGENlC [COP]/fUSCA [FUS]) that have pleiotropic effects, including repression of photomorphogenesis. Severa1 of these genes have now been cloned and characterized (reviewed in Chory et al, 1996;von Arnim and Deng, 1996). The proteins identified by this strategy include those that are involved in a diverse array of responses and not necessarily directly involved in mediating the action of phytochrome, but there is evidence that the DET7 and COP7 genes may play a specific role as repressors in photomorphogenesis (Chory et al, 1996;Wei and Deng, 1996).…”

Section: Introductionmentioning

confidence: 99%

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

et al. 1997

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We have isolated the gene for a protein designated CCAl. This protein can bind t o a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb7*3, which is necessary for its regulation by phytochrome. The CCAl protein interacted with two imperfect repeats in the Lhcb7*3 promoter, AAA/,AATCT, a sequence that is conserved in Lhcb genes. A region near the N terminus of CCA1, which has some homology t o the repeated sequence found in the DNA binding domain of Myb proteins, is required for binding t o the Lhcb7*3 promoter. Lines of transgenic Arabidopsis plants expressing antisense RNA for CCAl showed reduced phytochrome induction of the endogenous Lhcb7*3 gene, whereas expression of another phytochrome-regulated gene, rbcS-7A, which encodes the small subunit of ribulose-1 ,Bbisphosphate carboxylase/oxygenase, was not affected. Thus, the CCAl protein acts as a specific activator of Lhcb7*3 transcription in response t o brief red illumination. The expression of CCAl RNA was itself transiently increased when etiolated seedlings were transferred t o light. We conclude that the CCAl protein is a key element in the functioning of the phytochrome signal transduction pathway leading t o increased transcription of this Lhcb gene in Arabidopsis.

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“…One powerful approach to this problem is the isolation of mutants defective in normal light signaling (reviewed in Deng, 1994;Millar et al, 1994;McNellis and Deng, 1995;Quail et al, 1995;Chory et al, 1996;von Arnim and Deng, 1996). Several types of screens for mutants have been used.…”

Section: Introductionmentioning

confidence: 99%

SPA1: A New Genetic Locus Involved in Phytochrome A–Specific Signal Transduction

1998

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To identify mutants potentially defective in signaling intermediates specific to phytochrome A (phyA), we screened for extragenic mutations that suppress the morphological phenotype exhibited by a weak phyA mutant ( phyA-105 ) of Arabidopsis. A new recessive mutant, designated spa1 (for suppressor of phyA-105 ), was isolated and mapped to the bottom of chromosome 2. spa1 phyA-105 double mutants exhibit restoration of several responses to limiting fluence rates of continuous far-red light that are absent in the parental phyA-105 mutant, such as deetiolation, anthocyanin accumulation, and a far-red light-induced inability of seedlings to green upon subsequent transfer to continuous white light. spa1 mutations do not cause a phenotype in darkness, indicating that the suppression phenotype is light dependent. Enhanced photoresponsiveness was observed in spa1 seedlings in a wild-type PHYA background as well as in the mutant phyA-105 background but not in a mutant phyA null background. These results indicate that phyA is necessary in a non-allele-specific fashion for the expression of the spa1 mutant phenotype and that phyB to phyE are not sufficient for this effect. Taken together, the data suggest that spa1 mutations specifically amplify phyA signaling and therefore that the SPA1 locus encodes a component that acts negatively early in the phyA-specific signaling pathway.

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From seed germination to flowering, light controls plant development via the pigment phytochrome.

Cited by 191 publications

References 65 publications

In vitro culture of Chrysanthemum plantlets using light-emitting diodes

In vitro culture of Chrysanthemum plantlets using light-emitting diodes

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

SPA1: A New Genetic Locus Involved in Phytochrome A–Specific Signal Transduction

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