From Russia with lobe: genetic differentiation in trilobed uncus Ostrinia spp. follows food plant, not hairy legs

Фролов, А. Н.; Audiot, Philippe; Bourguet, Denis; Kononchuk, A.G.; Malysh, Julia M.; Ponsard, Sergine; Streiff, Réjane; Tokarev, Yuri S.

doi:10.1038/hdy.2011.58

Cited by 23 publications

(35 citation statements)

References 46 publications

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“…This may lessen stabilizing selection and create opportunities for pheromones to evolve, depending on the suite of selection forces a population is experiencing (21). Interestingly, field populations of the sibling taxon Ostrinia scapulalis (22), which has an identical pheromone polymorphism as O. nubilalis, were found in apparent Hardy-Weinberg equilibrium (23), although such situations have not been reported for O. nubilalis (24).…”

Section: Resultsmentioning

confidence: 95%

Early quality assessment lessens pheromone specificity in a moth

Kárpáti

Cardé

Dekker

2013

Proc. Natl. Acad. Sci. U.S.A.

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Pheromone orientation in moths is an exemplar of olfactory acuity. To avoid heterospecific mating, males respond to femaleproduced blends with high specificity and temporal resolution. A finely tuned sensory to projection neuron network secures specificity, and this network is thought to assess pheromone quality continually during orientation. We tested whether male moths do indeed evaluate each pheromone encounter and surprisingly found that male European corn borer moths instead generalize across successive encounters. Although initially highly ratio specific, once "locked on" to the pheromone plume the acceptable ratio can vary widely, and even unattractive blends can become attractive. We further found that this "mental shortcut" may be a consequence of the fact that sensory neurons exposed to frequent encounters do not reliably encode blend ratios. Neurons tuned to either of the two pheromone components adapt differentially in plumes containing the preferred blend ratio (97:3) and cause the olfactory sensory signal to "evolve," even in narrowly tuned pheromonal circuits. However, apparently the brain interprets these shifting signals as invariant "gestalts." Generalization in pheromone perception may mitigate stabilizing selection and allow introgression between sympatric strains, such as in the European corn borer, that otherwise appear isolated by pheromonal differences. Generalization may also be important in responses to general odorants, as circuits underlying these display vast sensitivity differences, complex interactions, and temporal intricacies.blend recognition | odor processing | sensory constraints | evolution | sexual selection

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Section: Resultsmentioning

confidence: 95%

Early quality assessment lessens pheromone specificity in a moth

Kárpáti

Cardé

Dekker

2013

Proc. Natl. Acad. Sci. U.S.A.

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“…1). Ранее экстракты геномной ДНК, полу-ченные для указанных объектов, были использованы для микросателлитного анализа, результаты которого позво-лили обосновать принадлежность насекомых с однодоль-ных и двудольных растений к двум близкородственным видам -O. nubilalis и O. scapulalis соответственно [14]. С целью проверки качества используемых экстрактов было проведено их тестирование методом ПЦР с прайме-рами LepF1 (5'-ATTCAACCAATCATAAAGATATTGG-3') : LepR1 (5'-TAAACTTCTGGATGTCCAAAAAATCA-3') [22] и 28sF3633 (5'-TACCGTGAGGGAAAGTTGAAA-3') : 28sR4076 (5'-AGACTCCTTGGTCCGTGTTT-3') [23], флан-кирующими фрагменты гена первой субъединицы митохондриальной цитохромоксидазы COI и субъ-единицы 28S рибосомной РНК (рРНК) соответствен-но.…”

Section: материалы и методыunclassified

“…Фертильность потомства от скрещивания особей из разных популяций резко снижена. В настоящее время эти попу-ляции разделены на два вида, имеющие общее происхождение, -Ostrinia scapulalis Muutura et Monroe и Ostrinia nubilalis Hübner, первый из которых считается предковой формой, а второй сформировался в результате адапта-ции к однодольным растениям [14]. Предполагается, что одним из факторов, повлиявших на процесс перехода насекомых с двудольных на однодольные растения, по-служили перепончатокрылые наездники.…”

Section: Introductionunclassified

Prevalence rates of Wolbachia endosymbiotic bacterium in natural populations of Ostrinia Nubilalis and Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) in South-Western Russia

Tokarev

Сергеевич²,

Yudina

et al. 2017

Ecological genetics

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Background. Endosymbiotic bacteria of the genus Wolbachia are widespread in arthropods and often cause reproductive abnormalities in lepidopteran insects, including corn borers of the genus Ostrinia. Wolbachia-Ostrinia is a promising model for studies of parasite-host interactions yet parasite prevalence in natural insect host populations remains unknown. Materials and Methods. Molecular genetic screening and statistical analysis is applied to evaluate prevalence rates of Wolbachia in sympatric populations of two corn borer species. Individual genomic DNA samples were extracted from last instar larvae collected in nature from different forage plants. For each sample of DNA showing positive signal with insect-specific primers the detection is performed using three diagnostic loci of Wolbachia: 16SrRNA, gatB and fbpA. Results. Wolbachia-positive signal is obtained for 13.5% larvae of Ostrinia nubilalis (N = 141) and 31.9% larvae of Ostrinia scapulalis (N = 138). In different localities the Wolbachia prevalence ranged from 2.9% (N = 34) to 65.8% (N = 38). Significantly higher rates of Wolbachia prevalence in insects from mugwort and hemp (O. scapulalis) as compared to those from corn (O. nubilalis) are revealed in three out of four localities. Conclusions. Endosymbiotic bacteria of the genus Wolbachia are revealed in natural populations of corn borers for the first time for Eastern Europe. The prevalence rates can be high and this should be taken into consideration when reproductive isolation is examined in population of these hosts as well as establishment of laboratory cultures is performed.

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“…The genetic and genomic architecture of the differentiation between O. scapulalis and O. nubilalis has been estimated based on various low-throughput tools: isozymes [16-19], genes [20], microsatellites [4,21], AFLPs [5]. Because of the restricted number of this type of markers and/or their anonymous location (microsatellites, AFLPs), there has been limited success in precisely identifying the genomic regions or genes implicated in the divergence between O. nubilalis and O. scapulalis .…”

Section: Introductionmentioning

confidence: 99%

“…Because of the restricted number of this type of markers and/or their anonymous location (microsatellites, AFLPs), there has been limited success in precisely identifying the genomic regions or genes implicated in the divergence between O. nubilalis and O. scapulalis . This divergence is rather low with remarkable exceptions at some markers [5,19,21], suggesting that some regions may carry genes involved in species differentiation and/or in speciation.…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis

et al. 2013

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BackgroundThis study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.ResultsBased on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes.ConclusionsThis data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes.

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From Russia with lobe: genetic differentiation in trilobed uncus Ostrinia spp. follows food plant, not hairy legs

Cited by 23 publications

References 46 publications

Early quality assessment lessens pheromone specificity in a moth

Early quality assessment lessens pheromone specificity in a moth

Prevalence rates of Wolbachia endosymbiotic bacterium in natural populations of Ostrinia Nubilalis and Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) in South-Western Russia

De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis

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