From Professor C. W. Helleiner

Helleiner, C. W.

doi:10.1016/0307-4412(75)90072-2

Cited by 5 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyacrylamide gel electrophoresis of the purified RNA preparations was carried out on 2.6% (w/v) gels as described previously (MEZEI and Hu, 1972). The radioactivity in the gel slices was counted by the methods of TISHLER and EPSTEIN (1968) or HELLEINER and WUNNER (1971). The two methods gave essentially the same results.…”

mentioning

confidence: 99%

Pattern of Rna Synthesis in the Sciatic Nerve of the Hen During Wallerian Degeneration

Muirhead

Mezei

1973

Journal of Neurochemistry

View full text Add to dashboard Cite

The pattern of synthesis of rapidly‐labelled RNA of hen sciatic nerve was studied during Wallerian degeneration. At 2,4,8, 16 and 30 days of degeneration the proximal and distal stumps of the severed nerve as well as the intact contralateral sciatic nerve (functional control) were excised and incubated with either [5‐3H]uridine or [2‐14C]uridine for 0.5 h. The electrophoretic pattern of RNA from the normal adult sciatic nerve showed that most of the radioactivity was incorporated into RNA species migrating between the 18 S and 4 S components of the bulk RNA. The synthesis of RNA was sensitive to actinomycin‐D, an indication that it was directed by a DNA template. The electrophoretic patterns of the rapidly‐labelled RNA in the proximal and distal nerve stumps demonstrated a change following nerve section. After 2–4 days of Wallerian degeneration the degenerating distal nerves incorporated more radioactivity in the 4 S region than the corresponding controls, but at 8 and 16‐days after degeneration relatively more label appeared in higher molecular weight RNA species. In the intact sciatic nerve of the operated hens progressively more radioactivity was detected in the 4 S region with increasing time after the contralateral nerve section. At each stage of Wallerian degeneration the specific radioactivities of RNA in the control nerves from experimental hens were higher than those of the normal adult sciatic nerve. These results indicated a change of RNA metabolism in increased functional activity and during Wallerian degeneration.

show abstract

mentioning

confidence: 99%

Pattern of Rna Synthesis in the Sciatic Nerve of the Hen During Wallerian Degeneration

Muirhead

Mezei

1973

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…However, there have been reports from Woods and associates (189,190) Attempts to isolate or intercept intermediates of the last step of methionine biosynthesis, such as the hypothetical S-hydroxymethylhomocysteine, have not yet led to clear-cut success (193)(194)(195). Thus, this phase of methionine biosynthesis, while established by tracer experiments, must await resolution of detail.…”

Section: B Conversion Of Homocysteine To Methioninementioning

confidence: 99%

The Biochemistry of Sulfonium Compounds

Shapiro

Schlenk

1960

Advances in Enzymology - And Related Areas of Molecular Biology

View full text Add to dashboard Cite

“…However, it is easy to believe that they were not too dissimilar to those documented by Sheela Surlekar at the National College of Chiropractic. Drawbacks to PBL, such as apprehension among staff and students, students not following very specific syllabuses, different students having different learning experiences, facilitators who lack confidence outside their own expertise, have all been mentioned as problems in this journal and elsewhere [see, for example [1][2][3][4][5][6][7][8][9]. In spite of these disadvantages, both papers record the experiences as successes!…”

mentioning

confidence: 99%

Problem-based learning

Smith

1998

Biochemical Education

View full text Add to dashboard Cite

From Professor C. W. Helleiner

Cited by 5 publications

References 0 publications

Pattern of Rna Synthesis in the Sciatic Nerve of the Hen During Wallerian Degeneration

Pattern of Rna Synthesis in the Sciatic Nerve of the Hen During Wallerian Degeneration

The Biochemistry of Sulfonium Compounds

Problem-based learning

Contact Info

Product

Resources

About