From phytoestrogens to obesity and the metabolic syndrome: Health from food and food for health

Virgili, Fabio; Perozzi, Giuditta

doi:10.1007/bf02829962

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…Monitoring and quantifying the amount of phytoestrogens in food is an important task, especially with regard to the daily sources of food for babies and growing children. In adolescent girls, phytoestrogens in dietary food appears to be responsible for the early onset of the menstrual cycle [9][10][11] due to the fact that they mimic the role of natural estrogen hormones. Thus, a wide variety of physical chemistry techniques have been applied to detect and quantify phytoestrogens in foods and biological fluids, including high-performance liquid chromatography (HPLC), 12,13 gas chromatography (GC), 14 capillary electrophoresis, 15 mass spectrosmetry 16 and other analytical methodologies.…”

Section: Introductionmentioning

confidence: 99%

Development of a nanoparticle-based FRET sensor for ultrasensitive detection of phytoestrogen compounds

Dumbrepatil¹,

Lee²,

Chung³

et al. 2010

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Phytoestrogens are plant compounds that mimic the actions of endogenous estrogens. The abundance of these chemicals in nature and their potential effects on health require the development of a convenient method to detect phytoestrogens. We have developed a nanoparticle (NP)-conjugated FRET probe based on the human estrogen receptor α (ER) ligand-binding domain (LBD) to detect phytoestrogens. The NP-conjugated FRET probe showed fluorescence signals for genistein, resveratrol and daidzein compounds with Δ ratios of 1.65, 2.60 and 1.37 respectively, which are approximately six times greater compared to individual FRET probes. A significantly higher signal for resveratrol versus genistein and daidzein indicates that the probe can differentiate between antagonistic phytoalexin substances and agonistic isoflavone compounds. NP-conjugated probes demonstrated a wide dynamic range, ranging from 10(-18) to 10(-1) M with EC(50) values of 9.6 × 10(-10), 9.0 × 10(-10) and 9.2 × 10(-10) M for genistein, daidzein and resveratrol respectively, whereas individual probes detected concentrations of 10(-13) to 10(-4) M for phytoestrogens compounds. The time profile revealed that the NP-conjugated probe is stable over 30 h and there is not a significant deviation in the FRET signal at room temperature. These data demonstrate that conjugation of a FRET probe to nanoparticles is able to serve as an effective FRET sensor for monitoring bioactive compounds with significantly increased sensitivity, dynamic range and stability.

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