From oleic acid-capped iron oxide nanoparticles to polyethyleneimine-coated single-particle magnetofectins

Cruz‐Acuña, Melissa; Maldonado-Camargo, Lorena; Dobson, Jon

doi:10.1007/s11051-016-3577-9

Cited by 10 publications

(9 citation statements)

References 40 publications

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“…Iron oxide magnetic nanoparticles were synthesized by the thermal decomposition method according to Cruz-Acuna et. al 11 . Briefly, formation of the iron-oleate complex was performed by dissolving ferric (III) chloride hexahydrate (98%, Sigma-Aldrich) in deionized water with sodium oleate (99%, Sigma-Aldrich), hexane, and ethanol and heated at 67 o C for 4 hours.…”

Section: Synthesis Of Magnetic Nanoparticles and Dispersion In Aqueoumentioning

confidence: 98%

“…For nanomagnetic transfection applications, polyethyleneimine (PEI) is often used to coat the surface of MNPs in order to optimise their functionality 10,11 . The presence of unbound PEI in MNP suspensions used for these applications is problematic as the excess PEI causes non-specific binding with DNA rather than DNA binding to the intended MNP surface, thus reducing the efficiency of nanomagnetic transfection 12 .…”

Section: Introductionmentioning

confidence: 99%

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Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions

Narayanasamy

Cruz‐Acuña

Everett

et al. 2018

Journal of Colloid and Interface Science

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Section: Synthesis Of Magnetic Nanoparticles and Dispersion In Aqueoumentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions

Narayanasamy

Cruz‐Acuña

Everett

et al. 2018

Journal of Colloid and Interface Science

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“…We compared the magnetically targeted GET-MNP system, with or without magnets to PEI. 44 PEI was slow to transfect and yielded ∼3-fold lower transfection levels in DC2.4 (dendritic cells) and ∼1.5-fold lower in HeLa cells (data not shown). GET-MNP transfection with 30 min magnetic targeting yielded higher levels of reporter expression than full transfection exposure (overnight) of the PEI-based systems (data not shown).…”

Section: Results and Discussionmentioning

confidence: 93%

“…We compared the magnetically targeted GET-MNP system, with or without magnets to PEI 44 . PEI was slow to transfect, and yielded ~3-fold lower transfection levels in DC2.4 (dendritic cells) and ~1.5-fold lower in HeLa cells (data not shown).…”

Section: Get Magnetofection Significantly Enhances Transfection Speedmentioning

confidence: 99%

Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles

Ferreras

Chan

Reina

et al. 2020

ACS Appl. Nano Mater.

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Non-viral delivery systems are generally of low efficiency, which limits their use in gene therapy and editing applications. We previously developed a technology termed glycosaminoglycan (GAG)-binding enhanced transduction (GET) to efficiently deliver a variety of cargos intracellularly; our system employs GAG-binding peptides, which promote cell targeting, and cell penetrating peptides (CPPs), which enhance endocytotic cell internalization. Herein, we describe a further modification by combining gene delivery and magnetic targeting with the GET technology. We associated GET peptides, plasmid (p)DNA, and iron oxide superparamagnetic nanoparticles (MNPs), allowing rapid and targeted GET-mediated uptake by application of static magnetic fields in NIH3T3 cells. This produced effective transfection levels (significantly higher than the control) with seconds to minutes of exposure and localized gene delivery two orders of magnitude higher in targeted over non-targeted cell monolayers using magnetic fields (in 15 min exposure delivering GFP reporter pDNA). More importantly, high cell membrane targeting by GET-DNA and MNP co-complexes and magnetic fields allowed further enhancement to endocytotic uptake, meaning that the nucleic acid cargo was rapidly internalized beyond that of GET complexes alone (GET-DNA). Magnetofection by MNPs combined with GET-mediated delivery allows magnetic field-guided local transfection in vitro and could facilitate focused gene delivery for future regenerative and disease-targeted therapies in vivo.

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“…The ease of manipulation of MNPs make them particularly attractive for in vivo applications, as researchers continue to seek methods to improve diagnosis and treatment non-invasively. Applications include the use of MNPs as contrast agents for magnetic resonance imaging (MRI) 1,2 , for drug and gene delivery [3][4][5] , magnetic separation of cells 6 , and hyperthermia [7][8][9] . As such for MNPs to be suitable for biological applications and safe in a clinical setting, they should exhibit biocompatibility, low toxicity, and chemical stability.…”

Section: Introductionmentioning

confidence: 99%

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Narayanasamy

Price

Merkhan

et al. 2019

Preprint

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The biocompatibility of surface coated/functionalised magnetic nanoparticles (MNPs) is key to their successful incorporation and application in biological systems. Polyethylene imine (PEI) -coated MNPs provide improved in vitro transfection efficiency compared to conventional chemical methods such as Lipofectamine and cationic polymers, and are also safer than viral transduction. Commercial cell toxicity assays are useful for end-point and high-throughput screening, providing fast results and an overview of cell health. However these assays only take into account cells that have undergone an extreme toxic response leading to cell death. Cell toxicity is a complex process which can be expressed in many forms, through morphological, metabolic, and epigenetic changes. A common indicator of cell stress and toxic response is increased cell adhesion and stress fibre formation. It is important to identify these changes in cells as it may affect downstream results and applications in biomedicine. This study explores the effect of the nanomagnetic transfection agent PEI-coated MNPs (MNP-PEIs) and an external magnetic field on cell behaviour, by studying particle internalization, changes in cellular morphology, and cell adhesion. We found that MNP-PEIs induced cell stress through a dose-dependent increase in cell adhesion via the overexpression of vinculin and formation of actin stress fibres. While the presence of PEI was the main contributor to increased cell stress, free PEI polyplexes induced higher toxicity compared to PEI bound to MNPs. MNPs without PEI coating however did not adversely affect cells suggesting a chemical effect instead of a mechanical one. In addition, genes identified as being associated with actin fibre regulation and cell adhesion, showed significant increases in expression due to the internalization of the MNP-PEI complex. From these results, we identify anomalous cell behaviour, morphology, and gene expression after interaction with MNP-PEIs, as well as a safe dosage to reduce acute cell toxicity.

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From oleic acid-capped iron oxide nanoparticles to polyethyleneimine-coated single-particle magnetofectins

Cited by 10 publications

References 40 publications

Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions

Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions

Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

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