From Molecules to Matrix: Construction and Evaluation of Molecularly Defined Bioscaffolds

Geutjes, Paul J.; Daamen, Willeke F.; Buma, P.; Feitz, W.F.J.; Faraj, Kaeuis A.; Kuppevelt, Toin H. Van

doi:10.1007/978-0-387-34133-0_19

Cited by 59 publications

(31 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, we have shown that it is possible to influence several biochemical and biomechanical parameters of molecularly defined scaffolds composed of collagen, elastin, glycosaminoglycans (including heparin, heparan sulfate, chondroitin sulfate, and dermatan sulfate), and growth factors (e.g., fibroblast growth factor 2 and vascular endothelial growth factor). 3,17,42 The preparation of biochemically and morphologically defined scaffolds may contribute to a rational design of scaffolds for tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Construction of Collagen Scaffolds That Mimic the Three-Dimensional Architecture of Specific Tissues

2007

Self Cite

View full text Add to dashboard Cite

Every tissue and organ has its own 3-dimensional (3D) extracellular matrix (ECM) organization. Cells in a 3D bioscaffold for tissue engineering typically align new ECM components according to the bioscaffold provided. Therefore, scaffolds with a specific 3D structural design resembling the actual ECM of a particular tissue may have great potential in tissue engineering. Here, we show that, using specific freezing regimes, 3D scaffolds that mimic the 3D architecture of specific tissues can be made from collagen. Three examples are given, namely, scaffolds resembling the cup-shaped parenchymal (alveolar) architecture of lung, scaffolds that mimic the parallel collagen organization of tendon, and scaffolds that mimic the 3D organization of skin. For the preparation of these tissue-specific scaffolds, we relied on simple techniques without the need for expensive or customized equipment. Freezing rate, type of suspension medium, and additives (e.g., ethanol) were found to be prime parameters in controlling scaffold morphology.

show abstract

Section: Discussionmentioning

confidence: 99%

Construction of Collagen Scaffolds That Mimic the Three-Dimensional Architecture of Specific Tissues

2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Type I collagen is a fibrous protein with highly repetitive amino acid sequences and is a candidate material for autograft. 12) Skin contains abundant type I collagen as ECM, and several roles of the collagen molecule in skin regeneration were previously reported. 13) In addition, both collagen and fibronectin could act as an important matrix protein for epithelial migration and proliferation.…”

Section: Resultsmentioning

confidence: 94%

Cell Shape and Matrix Production of Fibroblasts Cultured on Fibroin-organized Silk Scaffold with Type-II .BETA.-turn Structured (Ala-Gly-Ala-Gly-Ser-Gly)n Sequences

Hirata

Kobayashi

Matsumoto

et al. 2010

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Tissue engineering for skin regeneration is closely related to advances in carrier materials for fibroblast. To investigate the optimal fibroblast scaffold, we developed a silk protein containing multiple (AlaGly-Ala-Gly-Ser-Gly) n sequences, and used it for in vitro evaluations of cell shape, adhesion and matrix production. NIH-3T3 fibroblasts were cultured on silk scaffold and on control culture plates. At 2 hr, fibroblasts cultured on silk scaffold showed roundrose shape, but cells spread on control plates. The numbers of adhesive fibroblasts were the same in both scaffolds, indicating that silk scaffold affects cell shape but not adhesive efficiency. To examine the effects of silk scaffold on cytoskeletal changes of fibroblasts, cells were applied to actin staining. Fibroblasts cultured for 2 hr on silk scaffold showed dense actin fibers which formed ring-like structures, while actin stress fibers were formed in cells cultured on control plates. In a long-term culture of 14 days, piled matrices produced by fibroblasts have been shown distinctly in cells cultured on silk scaffold compared with cells cultured on control plates. The expression of fibronectin mRNA was elevated in fibroblasts cultured on silk scaffold compared with control, but the expression of collagens, type I and type III, mRNAs was similar in fibroblasts cultured on both scaffold. These results indicate that silk scaffold influences cell shape and actin fiber, and enhances matrix production with increased fibronectin. Therefore, silk protein may be an useful scaffold for regenerative therapy in various

show abstract

“…13 In brief, collagen was isolated from bovine achilles tendon using extractions with diluted acetic acid, sodium chloride, urea, and acetone solutions. A 0.67 wt% collagen suspension was prepared by mixing insoluble type I collagen with 0.25M acetic acid.…”

Section: Scaffold Preparation Contrast Agent Incorporation and Analmentioning

confidence: 99%

“…[12][13][14][15][16] We also have used collagen scaffolds to engineer urethral and bladder tissue in animal models. 17,18 The human urinary bladder wall can be visualized in T 2 -weighted MR images, because its signal intensity is low, while both the urine and the perivesical fat have high signal intensities.…”

mentioning

confidence: 99%

In VivoMagnetic Resonance Imaging of Type I Collagen Scaffold in Rat: Improving Visualization of Bladder and Subcutaneous Implants

Sun

Geutjes²,

Oosterwijk³

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Noninvasive monitoring of implanted scaffolds is important to understand their behavior and role in tissue engineering, in particular to follow their degradation and interaction with host tissue. Magnetic resonance imaging (MRI) is well suited for this goal, but its application is often hampered by the low contrast of scaffolds that are prepared from biomaterials such as type I collagen. The aim of this study was to test iron oxide particles incorporation in improving their MRI contrasts, and to follow their degradation and tissue interactions. Scaffolds with and without iron oxide particles were implanted either subcutaneously or on the bladder of rats. At predetermined time points, in vivo MRI were obtained and tissues were then harvested for histology analysis and transmission electron microscopy. The result showed that the incorporation of iron oxide particles improved MRI contrast of the implants, providing information on their location, shapes, and degradation. Second, the host tissue reaction to the type I collagen implants could be observed in both MRI and histology. Finally, MRI also revealed that the degradation and host tissue reaction of iron particles-loaded scaffolds differed between subcutaneous and bladder implantation, which was substantiated by histology.

show abstract

From Molecules to Matrix: Construction and Evaluation of Molecularly Defined Bioscaffolds

Cited by 59 publications

References 60 publications

Construction of Collagen Scaffolds That Mimic the Three-Dimensional Architecture of Specific Tissues

Construction of Collagen Scaffolds That Mimic the Three-Dimensional Architecture of Specific Tissues

Cell Shape and Matrix Production of Fibroblasts Cultured on Fibroin-organized Silk Scaffold with Type-II .BETA.-turn Structured (Ala-Gly-Ala-Gly-Ser-Gly)n Sequences

In VivoMagnetic Resonance Imaging of Type I Collagen Scaffold in Rat: Improving Visualization of Bladder and Subcutaneous Implants

Contact Info

Product

Resources

About