2017
DOI: 10.1111/pbi.12841
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From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

Abstract: SummaryBiomass yield, salt tolerance and drought tolerance are important targets for alfalfa (Medicago sativa L.) improvement. Medicago truncatula has been developed into a model plant for alfalfa and other legumes. By screening a Tnt1 retrotransposon‐tagged M. truncatula mutant population, we identified three mutants with enhanced branching. Branch development determines shoot architecture which affects important plant functions such as light acquisition, resource use and ultimately impacts biomass production… Show more

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Cited by 77 publications
(73 citation statements)
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References 65 publications
(86 reference statements)
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“…Earlier studies demonstrated that down-regulation of SPL8 (Squamosa Promoter-binding-Like protein-8) transcription factor significantly increased branching by promoting axillary bud development and enhanced forage biomass yield, besides enhancing salt and drought tolerance in alfalfa (66); these transgenic plants showed reduced GA accumulation, while spl8 mutants showed significantly higher GA transcript abundance. Furthermore, GA2-ox6, which is the prime GA deactivation enzyme, is significantly up-regulated by SPL8 (66). These results suggest that SPL8 regulates GA signaling, and GA2-ox is a possible key node of this signaling network (67).…”
Section: (C) Manipulation Of Phytohormone Levelsmentioning
confidence: 78%
“…Earlier studies demonstrated that down-regulation of SPL8 (Squamosa Promoter-binding-Like protein-8) transcription factor significantly increased branching by promoting axillary bud development and enhanced forage biomass yield, besides enhancing salt and drought tolerance in alfalfa (66); these transgenic plants showed reduced GA accumulation, while spl8 mutants showed significantly higher GA transcript abundance. Furthermore, GA2-ox6, which is the prime GA deactivation enzyme, is significantly up-regulated by SPL8 (66). These results suggest that SPL8 regulates GA signaling, and GA2-ox is a possible key node of this signaling network (67).…”
Section: (C) Manipulation Of Phytohormone Levelsmentioning
confidence: 78%
“…Total RNA samples from biological replicates of the selected transgenic events and the control were extracted from SAMs at the R 0 stage using Spectrum™ Plant Total RNA Kit (Sigma‐Aldrich). Microarray hybridization, data collection and analyses follow the procedures reported previously (Gou et al ., ).…”
Section: Methodsmentioning
confidence: 97%
“…In this work, six DERs that may be related to plant defense or morphogenesis were selected to construct the miRNA-target gene interaction network based on gene function annotation. Previous studies demonstrated that downregulation of squamosa promoter binding protein-like 8 (SPL8) could increase branch development by facilitating the formation of axillary buds [53] and regulate the GA signaling pathway and biosynthesis in stem elongation [54]. Gou et al [53] indicated that GA signaling transduction was regulated by SPL8 from the upstream GA receptor (GID1) to the downstream responsive genes (GRASs).…”
Section: Pawb Responsive Der-target Gene Pairs Regulate Morphologicalmentioning
confidence: 99%
“…Previous studies demonstrated that downregulation of squamosa promoter binding protein-like 8 (SPL8) could increase branch development by facilitating the formation of axillary buds [53] and regulate the GA signaling pathway and biosynthesis in stem elongation [54]. Gou et al [53] indicated that GA signaling transduction was regulated by SPL8 from the upstream GA receptor (GID1) to the downstream responsive genes (GRASs). GID1 sensed and bound endogenous GA to induce the formation of GID1-GA-DELLA protein complex [53].…”
Section: Pawb Responsive Der-target Gene Pairs Regulate Morphologicalmentioning
confidence: 99%
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