From microspheres to monoliths: Synthesis of porous supports with tailored properties by radiation polymerization

Grasselli, Mariano; Smolko, Eduardo E.; Hargittai, P.; Sáfrány, Ágnes

doi:10.1016/s0168-583x(01)00839-4

Cited by 42 publications

(30 citation statements)

References 15 publications

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“…Sáfrány et al [47] from the institute of Surface Chemistry in Budapest (Hungary) have developed an innovative polymerization procedure. The copolymerization is prompted by exposure to 60 Co g-radiation.…”

Section: Researchmentioning

confidence: 99%

“…Grasselli, Sáfrány et al [47] Preparation of a porous polymethacrylate-based resin: -Monomers: diethylene glycol dimethacrylate (DEGDMA) and GMA at various concentrations -Porogen: variable (ethylpropionate, alcohols, acetone, THF, dioxane) Polymerization initiated with radiations produced by a 60 Co g source at various dose rates (total doses ranging from 1 to 50 kGy) and temperatures…”

Section: We Thankmentioning

confidence: 99%

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Advances in capillary electrochromatography and micro‐high performance liquid chromatography monolithic columns for separation science

et al. 2003

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Over the last decade, monoliths or continuous beds have emerged as an alternative to traditional packed-bed columns for use in capillary electrochromatography (CEC) and micro-high performance liquid chromatography (micro-HPLC). Monolithic columns can be divided into two categories: silica-based monolithic columns and rigid organic polymer-based monolithic columns resulting from the polymerization of acrylamide, styrene, acrylate or methacrylate monomers. In this paper, the chemistry and most recent applications of these various types of monoliths in both CEC and micro-HPLC are presented.

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Section: Researchmentioning

confidence: 99%

Section: We Thankmentioning

confidence: 99%

Advances in capillary electrochromatography and micro‐high performance liquid chromatography monolithic columns for separation science

et al. 2003

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“…Synthesis of monolithic supports was based on a previously published procedure [25]. Fused-silica capillaries (Polymicro Technologies, Phoenix, AZ, USA) of 10 cm length (150 mm ID, 365 mm OD) were filled with deoxygenated solutions containing 40% v/v mixture of DEGDMA and GMA monomers in ethanol.…”

Section: Preparation Of Capillariesmentioning

confidence: 99%

“…Radiation-initiated in situ polymerization and cross-linking of the monolith in fused-silica capillaries and subsequent modification of the monolith surface were performed according to published procedures [25,26]. The resulting columns were examined by SEM to visualize the structure of the synthesized polymeric support (Fig.…”

Section: Monolith Structure and Surface Modificationmentioning

confidence: 99%

“…Such thermal initiation presents the disadvantage of pore-size dependence on temperature, while photoinitiation can be used only for monoliths of limited size [24]. An alternative method is the synthesis of monoliths by g-radiation-initiated polymerization and cross-linking [25]. Ionizing radiations produce free radicals when acting on organic compounds.…”

Section: Introductionmentioning

confidence: 99%

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On-line affinity selection of histidine-containing peptides using a polymeric monolithic support for capillary electrophoresis

et al. 2005

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An on-line affinity selection method using a polymeric monolithic support is proposed for the retention of histidine-containing peptides and their subsequent separation by capillary zone electrophoresis (CZE). Monolithic capillary columns were prepared in fused-silica capillaries of 150 mum inner diameter (ID) by ionizing radiation-initiated in situ polymerization and cross-linking of diethylene glycol dimethacrylate and glycidyl methacrylate, and chemically modified with iminodiacetic acid (IDA) and copper ion. Monolithic microextractors were coupled on-line near the inlet of the separation capillary (fused-silica capillary, 75 mum ID x 28 cm from the microextractor to the detector). Model peptide mixtures of histidine-containing and histidine-noncontaining peptides were assessed. Peptides were released from the sorbent by a 5 mM imidazole solution and then separated by CZE with ultraviolet detection. Relative standard deviation values for migration times and corrected peak areas were found to be lower than 5.8 and 10.5%, respectively. IDA-Cu(II) ion modified monolithic microextractors showed a chromatographic behavior and could be reused at least 25 times. The use of monolithic supports proved to be an advantageous alternative to packed particles for the preparation of microextractors.

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Monolithic High‐Performance Liquid Chromatography Columns

Desire

Hilder

Arrua

2017

Encyclopedia of Analytical Chemistry

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Monolithic HPLC (high‐performance liquid chromatography) columns have emerged over the recent decades as highly attractive alternatives to conventional HPLC technology. A monolith is considered to be a continuous piece of porous material. Two types of monolithic columns have been developed for liquid chromatography (LC) applications; they are polymer monoliths and silica monoliths, which differ based on their porous properties. Polymer monoliths consist solely of macropores (pores larger than 50 nm), while silica monoliths possess both macropores and mesopores (pores between 2 and 50 nm). Silica monoliths therefore possess higher surface areas than polymer monoliths and have mainly been utilied for the separation of small molecules, where excellent separation performance has been demonstrated. Polymer monoliths on the other hand have been shown to provide excellent separation performance for larger molecules, where the convective transport of these molecules is unhindered by the presence of mesopores. Polymer and silica monoliths can be prepared in a variety of ways, resulting in varied surface chemistries and flow properties, thus allowing their application in a wide range of chromatographic modes, including reversed‐phase (RP), ion‐exchange, hydrophilic interaction, and hydrophobic interaction chromatography (HIC), to name a few. This has allowed for the separation of a variety of analytes, from small molecules such as alkylbenzenes, to larger molecules such as peptides and proteins. They have also found application for the separation of DNA. In recent years, significant attention has been focused on improving and understanding the behavior of these materials and alternative synthetic routes for their preparation.

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From microspheres to monoliths: Synthesis of porous supports with tailored properties by radiation polymerization

Cited by 42 publications

References 15 publications

Advances in capillary electrochromatography and micro‐high performance liquid chromatography monolithic columns for separation science

Advances in capillary electrochromatography and micro‐high performance liquid chromatography monolithic columns for separation science

On-line affinity selection of histidine-containing peptides using a polymeric monolithic support for capillary electrophoresis

Monolithic High‐Performance Liquid Chromatography Columns

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