From micro to nano: recent advances in high-resolution microscopy

Garini, Yuval; Vermolen, Bart J.; Young, Ian T.

doi:10.1016/j.copbio.2005.01.003

Cited by 140 publications

(84 citation statements)

References 43 publications

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“…Fluorescence microscopy has proven to be an invaluable method for imaging in cell biology, primarily due to its high sensitivity and the wide range of probes available for the selective labelling of cellular structures [2]. While conventional fluorescence microscopy is ultimately limited in the spatial resolution achievable (diffraction limited to ~200nm), spatial resolution can be improved by using point scanning methods such as confocal microscopy [3], and other sophisticated microscopy developments such as multiphoton, stimulated emission depletion, 4-Pi, image interference and near-field scanning microscopy [4].…”

Section: Introductionmentioning

confidence: 99%

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

Millington

Grindlay

Altenbach

et al. 2007

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT High-Precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion proteinMichael Millington, [a, b] G. Joan Grindlay, [c] Kirsten Altenbach, [a, d] Robert K. Neely, [a, b] Walter Kolch, [ c, e] Mojca Bencina , [ f ] Nick D. Read, [a, d] Anita C. Jones, [a, b] David T. F.Dryden, [a, b]

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Section: Introductionmentioning

confidence: 99%

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

Millington

Grindlay

Altenbach

et al. 2007

Biophysical Chemistry

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“…Advances in molecular biology, organic chemistry, and materials science have resulted in an impressive toolbox of fluorescent proteins (GFP and variants) and nanocrystals (quantum dots) and have enabled the study of protein expression, localisation, conformation, diffusion, turnover, trafficking, and interaction (Giepmans et al 2006;Lippincott-Schwartz and Patterson 2003). Hardware advances in optical systems design have taken light microscopy from wide field to (multiphoton) confocal and spinning disk microscopy (Stephens and Allan 2003), and more recent efforts to break the diffraction barrier have further extended the palette (Garini et al 2005;Hell 2009). Together, these developments have redefined biological research by enabling the switch from fixed to living cells and from qualitative to quantitative imaging (Tsien 2003).…”

Section: Live-cell Imagingmentioning

confidence: 99%

In vitro study methodologies to investigate genetic aspects and effects of drugs used in attention-deficit hyperactivity disorder

Grünblatt

Bartl²,

Marinova

et al. 2012

J Neural Transm

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Attention-deficit/hyperactivity disorder (ADHD) is one of the most common psychiatric disorders in children and adolescents, with up to 5 % affected worldwide. Twin and family studies on ADHD show its high familiality with heritability estimated around 70 %, but, to date, no specific polymorphism or gene was found to be specifically affected. Psychostimulants (amphetamine, methylphenidate) and non-psychostimulants (atomoxetine) are used successfully in ADHD therapy, but many of their mechanisms of action and their adverse effects are not yet fully understood. Therefore, both genetic findings and therapeutic interventions should be further investigated. One easy platform for such studies is in vitro analyses, which encompass neuronal cell culture studies, transfections of genetic constructs, binding and electrophysiology analyses. In this review, different methods will be referred in particular to ADHD findings, and new techniques will be mentioned for future studies of drug or genetic effects in vitro. DOI: https://doi.org/10.1007/s00702-012-0869-9Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-74484 Accepted Version Originally published at: Grünblatt, Edna; Bartl, Jasmin; Marinova, Zoya; Walitza, Susanne (2013). In vitro study methodologies to investigate genetic aspects and effects of drugs used in attention-deficit hyperactivity disorder. Journal of Neural Transmission, 120 (1)

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“…The transition from two-dimensional (2D) to threedimensional (3D) imaging has allowed us to better understand how the nucleus is spatially organized. In addition to 3D approaches, live cell imaging has added a new dimension to our ability of developing clear concepts about the dynamics of nuclear organization (Liu and Chang, 2003;Garini et al, 2005). Live cell imaging with 3D resolution is often called fourdimensional (4D) imaging, where the fourth dimension of time is added to the imaging in the x,y, and z planes, that constitute a 3D image.…”

Section: Imaging Of Nuclear Structuresmentioning

confidence: 99%

Three-dimensional organization of the mammalian nucleus in normal and tumor cells

Mai¹

2011

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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From micro to nano: recent advances in high-resolution microscopy

Cited by 140 publications

References 43 publications

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

In vitro study methodologies to investigate genetic aspects and effects of drugs used in attention-deficit hyperactivity disorder

Three-dimensional organization of the mammalian nucleus in normal and tumor cells

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