From Micro to Macro: The Hierarchical Design in a Micropatterned Scaffold for Cell Assembling and Transplantation

Wang, Kai; Wang, Xi; Han, Chengsheng; Hou, Wenhua; Wang, Jinyang; Chen, Liangyi; Luo, Ying

doi:10.1002/adma.201604600

Cited by 44 publications

(39 citation statements)

References 56 publications

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“…In comparison, cell clumping and central necrosis were observed in the islets dispersed in tissue culture plates. This is probably because the absence of ECM lead to the necrosis of the dispersed islets leading to poor islet survival and transplantation failure . In addition, islets were also encapsulated using a positively charged P‐Alg/PEI hydrogel, but the islets were all dead after 24 h culture, probably due to the excess positive charges in the hydrogel could damage the cells (Figure S3, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Rapid and Long‐Term Glycemic Regulation with a Balanced Charged Immune‐Evasive Hydrogel in T1DM Mice

Zhang

Zhu

Song

et al. 2019

Adv Funct Materials

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Transplantation of encapsulated islets is a promising treatment for patients with type 1 diabetes mellitus. However, its long‐term clinical therapeutic efficacy is still hindered by serious immune rejection from the host immunological responses to the implanted materials, known as foreign body reaction (FBR). In this work, an anti‐biofouling balanced charged hydrogel is reported that can serve as an excellent immunoprotective material for islet transplantation therapy. It is found that the encapsulated islets can maintain their glucose‐responsive and insulin‐producing functions. Additionally, the hydrogel can effectively evade in vivo FBR after intraperitoneal implantation in an immunocompetent streptozotocin‐induced diabetic mouse model. As a result, 100% of the mice rapidly recover to normoglycemia within 2 d and stably maintain for at least 150 d without any immunosuppression treatment. These findings shed light on the “insulin independence and immunoisolation” encapsulation strategy, which can overcome the barrier of islet transplantation and holds the potential to improve current clinical therapeutic efficacy.

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Section: Resultsmentioning

confidence: 99%

Rapid and Long‐Term Glycemic Regulation with a Balanced Charged Immune‐Evasive Hydrogel in T1DM Mice

Zhang

Zhu

Song

et al. 2019

Adv Funct Materials

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“…The nanofibrous material allows proper diffusion of nutrients across it and assists in cell adhesion. The combination of 3D spheroids with the electrospun fibers showed improved cell functions in rodent models . A recent study has demonstrated that the sophisticated double 3D strategy could increase the efficiency of implants even in the absence of bioactive molecules.…”

Section: Recent Advancesmentioning

confidence: 99%

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

Sankar

Sharma

Rath

et al. 2017

Biotechnology Journal

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Electrospinning is a popular technique used to mimic the natural sub-micron features of the native tissue. The ultra-fine fibers provide a favorable extracellular matrix-like environment for regulation of cellular functions. This article summarizes and reviews the current advances in electrospun fiber application and focuses on the novel strategies applied for tissue regeneration and repair. It explores the different factors affecting the attachment and proliferation of mesenchymal stem cells (MSCs) on the electrospun substrates. The influence of different features of electrospun fibers in the differentiation of MSCs into specific lineages (bone, cartilage, tendon/ligament, and nerves) has been elaborated. In addition, the different techniques to mimic the hierarchical features of tissues and its effect on cellular functions are reviewed. Additionally, the new developments like three-dimensional (3D) electrospinning, 3D spheroid double strategy and the comparative analysis of dynamic and static culture on electrospun scaffolds are discussed. With the intricate understanding of the interaction between the cells and the electrospun fiber matrix we can aim to combine the newer strategies to overcome the existing challenges and improve the potential application of electrospun fibers in the field of tissue regeneration and repair.

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“…However, most efforts are centered on static patterns. [ 6,7 ] Inspired by dynamic, tissue‐specific microenvironments in vivo, the use of dynamic cell culture platforms to create synthetic microenvironments in vitro has become an attractive means to direct active changes in cellular functionality and to replicate the dynamic complexity of native tissues. [ 8,9,10 ]…”

Section: Introductionmentioning

confidence: 99%

“…[ 16 ] 3D cell aggregates can closely resemble the native configuration of NSCs in vivo, which allows for direct cell–cell signaling and cell–matrix interactions when compared to 2D culture substrates or 3D scaffolds. [ 7,18 ] Several studies have induced NSC aggregation using a cell spheroid technique. However, the generation of conventional cell spheroids typically involves a complicated culturing process, which has been criticized for being sensitive to cell culture conditions, and often results in inconsistently differentiated cells, even between experimental trials.…”

Section: Introductionmentioning

confidence: 99%

4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation

et al. 2020

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As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research.

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From Micro to Macro: The Hierarchical Design in a Micropatterned Scaffold for Cell Assembling and Transplantation

Cited by 44 publications

References 56 publications

Rapid and Long‐Term Glycemic Regulation with a Balanced Charged Immune‐Evasive Hydrogel in T1DM Mice

Rapid and Long‐Term Glycemic Regulation with a Balanced Charged Immune‐Evasive Hydrogel in T1DM Mice

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation

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