From mass cytometry to cancer prognosis

Winter, Deborah R.; Ledergor, Guy; Amit, Ido

doi:10.1038/nbt.3346

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…Mass cytometry, although a very recently developed technology , has already begun to have significant impact on molecular, cell and clinical science . It shares many similarities with flow cytometry, in that a mass cytometer is able to perform high‐throughput multiparametric single cell analysis.…”

Section: Introductionmentioning

confidence: 99%

Cell size assays for mass cytometry

2016

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Mass cytometry offers the advantage of allowing the simultaneous measurement of a greater number parameters than conventional flow cytometry. However, to date, mass cytometry has lacked a reliable alternative to the light scatter properties that are commonly used as a cell size metric in flow cytometry (forward scatter intensity—FSC). Here, we report the development of two plasma membrane staining assays to evaluate mammalian cell size in mass cytometry experiments. One is based on wheat germ agglutinin (WGA) staining and the other on Osmium tetroxide (OsO4) staining, both of which have preferential affinity for cell membranes. We first perform imaging and flow cytometry experiments to establish a relationship between WGA staining intensity and traditional measures of cell size. We then incorporate WGA staining in mass cytometry analysis of human whole blood and show that WGA staining intensity has reproducible patterns within and across immune cell subsets that have distinct cell sizes. Lastly, we stain PBMCs or dissociated lung tissue with both WGA and OsO4 ; mass cytometry analysis demonstrates that the two staining intensities correlate well with one another. We conclude that both WGA and OsO4 may be used to acquire cell size-related parameters in mass cytometry experiments, and expect these stains to be broadly useful in expanding the range of parameters that can be measured in mass cytometry experiments.

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Section: Introductionmentioning

confidence: 99%

Cell size assays for mass cytometry

2016

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“…This modification process can be characterized by multiple means. To understand the contents of the DNA attached to the nanoprobe we added for each sample, we used the high-resolution ICPMS (HR-ICPMS) to quantitatively analyze 31 P on the nanoprobes. 51 We use the same length of DNA as used for a standard curve of the P element, then make a quantity of probe DNA (Figure 2c), and it is concluded that each sample substantially corresponds to 8 pmol of probe added, with a similar connecting efficiency of each NP.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Elemental mass spectrometry, especially inductively coupled plasma mass spectrometry (ICPMS), 26 − 28 is emerging as a powerful platform for the multiplex bioassay 29 − 31 due to its high sensitivity, 32 wide dynamic range, 33 and multiplex sensing ability. 34 − 36 The multiplex bioassay by ICPMS 37 possesses many superiorities, such as a low sample consumption, 38 a short analysis time, a simplified procedure, and a low cost.…”

mentioning

confidence: 99%

“…Elemental mass spectrometry, especially inductively coupled plasma mass spectrometry (ICPMS), − is emerging as a powerful platform for the multiplex bioassay − due to its high sensitivity, wide dynamic range, and multiplex sensing ability. − The multiplex bioassay by ICPMS possesses many superiorities, such as a low sample consumption, a short analysis time, a simplified procedure, and a low cost. − Zhang’s group developed a multiplex nucleic acid assay and immunoassay by lanthanide macrocyclic compound tagging. , Wang et al performed a multiplex assay for protease markers . Simultaneous detection of multiple microRNAs by the MNAzyme-catalyzed amplification assay was realized, while single-particle mode ICPMS was applied for a three-component homogeneous immunoassay of pancreatic cancer .…”

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confidence: 99%

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Multiplex Nucleic Acid Assay of SARS-CoV-2 via a Lanthanide Nanoparticle-Tagging Strategy

Chen

Huang

et al. 2021

Anal. Chem.

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Early diagnosis, early isolation, and early treatment are efficient solutions to control the COVID-19 pandemic. To achieve the accurate early diagnosis of SARS-CoV-2, a multiplex detection strategy is required for the cross-validation to solve the problem of “false negative” of the existing gold standard assay. Here, we present a multicomponent nucleic acid assay platform for SARS-CoV-2 detection based on lanthanide nanoparticle (LnNP)-tagging strategy. For targeting SARS-CoV-2’s RNA fragments ORF1ab gene, RdRp gene, and E gene, three LnNP probes can be used simultaneously to identify three sites in one sample through elemental mass spectrometry detection with limits of detection of 1.2, 1.3, and 1.3 fmol, respectively. With the multisite cross-validation, we envision that this multiplex and sensitive detection platform may provide an effective strategy for SARS-CoV-2 fast screening with a high accuracy.

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“…An example to illustrate the importance of somatic heterogeneity is cancer. Here, analysis may reveal the existence of rare transformed individual cells, differences in driver mutations, and diverse resistance mechanisms in response to treatment [ 4 , 5 , 6 , 7 , 8 , 9 ]. There is also a great interest in the identification of somatic structural variations in normal tissues—in particular, in the brain [ 10 ].…”

Section: Introductionmentioning

confidence: 99%