2018
DOI: 10.1002/pep2.24041
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From liposomes to cells: Filling the gap between physicochemical and microbiological studies of the activity and selectivity of host‐defense peptides

Abstract: Host‐defense peptides (HPDs) are bactericidal and immunomodulatory molecules, part of the innate immune system of many organisms, including man. They kill bacteria mostly by perturbing their membranes, and for this reason they are a promising class of molecules to fight drug‐resistant microbes. However, their success towards clinical application is still limited, partly due to many unanswered questions on their activity and function. Our current understanding of HDPs has been reached by two parallel, but large… Show more

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Cited by 40 publications
(77 citation statements)
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References 134 publications
(225 reference statements)
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“…Our quantification of peptide retention (~3.8 × 10 7 peptides/cell) is within the range reported using various methods Steiner et al (1988); Savini et al (2018); Starr et al (2016); Tran et al (2002); Melo et al (2011); Roversi et al (2014). Yet, this large number raises the question of which molecules inside of the cells are interacting with LL37.…”
Section: Discussionsupporting
confidence: 82%
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“…Our quantification of peptide retention (~3.8 × 10 7 peptides/cell) is within the range reported using various methods Steiner et al (1988); Savini et al (2018); Starr et al (2016); Tran et al (2002); Melo et al (2011); Roversi et al (2014). Yet, this large number raises the question of which molecules inside of the cells are interacting with LL37.…”
Section: Discussionsupporting
confidence: 82%
“…At the cellular and molecular scales, a distinct feature of the AMP’s mechanism of action is its collective nature, where a large number of AMPs are required to first bind to and then disrupt the cell membranes to kill the cell. AMP’s absorption has been discussed and quantified previously for different microorganisms and peptides using various techniques Steiner et al (1988); Savini et al (2018); Starr et al (2016); Tran et al (2002); Melo et al (2011); Roversi et al (2014). Utilizing live, single-cell microscopy, we observed the temporal and cell-to-cell heterogeneity in the peptide absorption into E. coli cells, which goes beyond the membrane binding.…”
Section: Discussionmentioning
confidence: 72%
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“…In addition, cancer cells have a higher content of other anionic moieties on their surface, such as sialic acid and heparan sulphate [3,[18][19][20]. A net negative charge is a property shared by bacterial membranes, which contain significant fractions of the anionic lipids phosphatidylglycerol (PG) and cardiolipin (CL); additional negative moieties are present in other components of the cell envelope (teichoic and teichuronic acids and lipopolysaccharides) [8,21]. The cationic charge of HDPs is an obvious determinant of their selectivity.…”
Section: Introductionmentioning
confidence: 99%