From Junk to Function: LncRNAs in CNS Health and Disease

Policarpo, Rafaela; Sierksma, Annerieke; Strooper, Bart De; d’Ydewalle, Constantin

doi:10.3389/fnmol.2021.714768

Cited by 34 publications

(26 citation statements)

References 243 publications

(348 reference statements)

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“…In post-transcriptional regulation, lncRNAs are implicated in the stability and translation of mRNAs, pre-mRNA splicing, protein activities, and act as precursors of miRNAs and siRNAs [39,40]. In epigenetic level, lncRNAs regulate gene expression through DNA methylation or demethylation, RNA interference (RNAi), histone modification and chromosome remodeling [38,39,41]. In the last decades, plenty of studies on lncRNAs have been conducted in mammals.…”

Section: Introductionmentioning

confidence: 99%

Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA

Zhu

Wei

et al. 2021

PLoS Genet

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The evolution of resistance to insecticides is well known to be closely associated with the overexpression of detoxifying enzymes. Although the role of glutathione S-transferase (GST) genes in insecticide resistance has been widely reported, the underlying regulatory mechanisms are poorly understood. Here, one GST gene (GSTu1) and its antisense transcript (lnc-GSTu1-AS) were identified and cloned, and both of them were upregulated in several chlorantraniliprole-resistant Plutella xylostella populations. GSTu1 was confirmed to be involved in chlorantraniliprole resistance by direct degradation of this insecticide. Furthermore, we demonstrated that lnc-GSTu1-AS interacted with GSTu1 by forming an RNA duplex, which masked the binding site of miR-8525-5p at the GSTu1-3′UTR. In summary, we revealed that lnc-GSTu1-AS maintained the mRNA stability of GSTu1 by preventing its degradation that could have been induced by miR-8525-5p and thus increased the resistance of P. xylostella to chlorantraniliprole. Our findings reveal a new noncoding RNA-mediated pathway that regulates the expression of detoxifying enzymes in insecticide-resistant insects and offer opportunities for the further understanding of the mechanisms of insecticide and drug resistance.

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Section: Introductionmentioning

confidence: 99%

Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA

Zhu

Wei

et al. 2021

PLoS Genet

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“…Long noncoding RNAs (lncRNAs) are a type of non-coding RNA defined as transcripts >200 nucleotides that lack the potential to encode proteins and are involved in the regulation of a variety of tumors 18,19 . In addition, some lncRNAs are closely associated with drug resistance in osteosarcoma cells 20 .…”

Section: Discussionmentioning

confidence: 99%

Lnc BACE1-AS Promotes The Progression of Osteosarcoma Through miR-762/SOX7 Axis

Wang

Zhang

et al. 2021

Preprint

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Background: Osteosarcoma (OS) is a rare malignant primary tumor of mesenchymal origin affecting bone that occurs in adolescents and children. LncRNAs are important regulators of tumorigenesis and development. This study aimed to explore the role and molecular basis of LncRNA BACE1-AS in OS. Methods and results : Through the analysis of differential expressed lncRNAs in OS tissues by GEO database, LncRNA BACE1-AS displayed a remarkably lower expression. This found could also be observed in both OS tissues and cell lines by qRT-PCR. Furthermore, using Cell counting kit-8 (CCK-8), transwell, wound healing and westernblot assays, overexpression LncRNA BACE1-AS remarkably reduced cell proliferation, migration and invasion abilities in OS. In addition, LncRNA BACE1-AS was validated as a sponge of miR-762 through the prediction of lncRNASNP. Further, luciferase reporter and RIP assays were conducted to confirm the binding sites between LncRNA BACE1-AS and miR-762. SOX7 was a target of miR-762 and could be regulate by LncRNA BACE1-AS. Moreover, inhibition of miR-762 could attenuate the role of sh-LncRNA BACE1-AS in OS cells, at meanwhile reduced the expression of SOX7. Conclusion : In this study, LncRNA BACE1-AS regulated proliferation, migration, invasion and apoptosis of OS cells by miR-762/SOX7 axis, implying that LncRNA BACE1-AS was a potential target for OS therapy.

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“…While these non-coding RNAs lack obvious protein-coding potential, they appear to play crucial cellular functions; many of them have been identified as novel regulators of gene expression at the epigenetic, transcriptional, post-transcriptional, and translational levels [ 28 ]. Amongst these, long non-coding RNAs (lncRNAs), a subclass of non-coding RNAs typically longer than 200 nucleotides, have been shown to participate in brain development and function, and the dysregulation of their expression implicated in many neurological disorders [ 29 ]. In particular, aberrant expression of many lncRNAs has been linked to AD [ 30 , 31 ].…”

Section: Alzheimer’s Disease and The Non-coding Genome: What Is The Link?mentioning

confidence: 99%

“…LncRNAs may represent an additional and attractive novel class of biomarkers for several reasons. First, they exhibit highly regulated spatiotemporal expression patterns, particularly in the brain and spinal cord [ 24 , 29 , 81 ]. Second, many lncRNA are differentially expressed in the brain during progression of various neurodegenerative disorders [ 29 , 30 ].…”

Section: Long Non-coding Rnas: a Diagnostic Tool For Alzheimer’s Disease?mentioning

confidence: 99%

Missing lnc(RNAs) in Alzheimer’s Disease?

Policarpo

d’Ydewalle

2021

Genes

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With the ongoing demographic shift towards increasingly elderly populations, it is estimated that approximately 150 million people will live with Alzheimer’s disease (AD) by 2050. By then, AD will be one of the most burdensome diseases of this and potentially next centuries. Although its exact etiology remains elusive, both environmental and genetic factors play crucial roles in the mechanisms underlying AD neuropathology. Genome-wide association studies (GWAS) identified genetic variants associated with AD susceptibility in more than 40 different genomic loci. Most of these disease-associated variants reside in non-coding regions of the genome. In recent years, it has become clear that functionally active transcripts arise from these non-coding loci. One type of non-coding transcript, referred to as long non-coding RNAs (lncRNAs), gained significant attention due to their multiple roles in neurodevelopment, brain homeostasis, aging, and their dysregulation or dysfunction in neurological diseases including in AD. Here, we will summarize the current knowledge regarding genetic variations, expression profiles, as well as potential functions, diagnostic or therapeutic roles of lncRNAs in AD. We postulate that lncRNAs may represent the missing link in AD pathology and that unraveling their role may open avenues to better AD treatments.

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From Junk to Function: LncRNAs in CNS Health and Disease

Cited by 34 publications

References 243 publications

Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA

Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA

Lnc BACE1-AS Promotes The Progression of Osteosarcoma Through miR-762/SOX7 Axis

Missing lnc(RNAs) in Alzheimer’s Disease?

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