From Germ Cell Preservation to Regenerative Medicine: An Exciting Research Career in Biotechnology

Wilmut, Ian

doi:10.1146/annurev-animal-022513-114214

Cited by 2 publications

(2 citation statements)

References 59 publications

(48 reference statements)

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“…Extracellular ice also directly damages the cells by puncturing or crushing cell membranes [ 19 ]. The species and cell type specific proper cooling rates are therefore crucial in a way that cooling too quickly leads to lethal intracellular ice formation, whereas cooling too slowly makes the cells suffering lethal “solution damage” [ 20 ].…”

Section: Semenmentioning

confidence: 99%

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Sun

Zong

et al. 2022

J Animal Sci Biotechnol

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Poultry genetics resources, including commercial selected lines, indigenous breeds, and experimental lines, are now being irreversibly lost at an alarming rate due to multiple reasons, which further threats the future livelihood and academic purpose. Collections of germplasm may reduce the risk of catastrophic loss of genetic diversity by guaranteeing that a pool of genetic variability is available to ensure the reintroduction and replenishment of the genetic stocks. The setting up of biobanks for poultry is challenging because the high sensitiveness of spermatozoa to freezing–thawing process, inability to cryopreserve the egg or embryo, coupled with the females being heterogametic sex. The progress in cryobiology and biotechnologies have made possible the extension of the range of germplasm for poultry species available in cryobanks, including semen, primordial germ cells, somatic cells and gonads. In this review, we introduce the state-of-the-art technologies for avian genetic resource conservation and breed reconstruction, and discuss the potential challenges for future study and further extending of these technologies to ongoing and future conservation efforts.

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Section: Semenmentioning

confidence: 99%

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Sun

Zong

et al. 2022

J Animal Sci Biotechnol

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“…DMA freezing technology relies on its ability to penetrate the sperm and replace its intracellular water to prevent the damage caused by ice crystal formation during the freezing/thawing process [ 15 ]. In previous research, DMA has been included in semen extenders at a concentration ranging from 3% to 26%, either to examine or to improve the efficiency of chicken sperm cryopreservation with or without other supplementations [ 4 , 10 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm

Mehaisen

Elomda

Hamad

et al. 2022

Animals

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Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility of poultry sperm to cryodamage leads to low fertility rates after cryopreservation. Therefore, the present study aimed at evaluating the effect of including a cryoprotectant, dimethylacetamide (DMA), in the chicken semen freezing extenders at a final concentration of 3%, 6%, or 9% on the post-thawed sperm motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed that the total motile sperm, progressivity, and viability were quadratically increased (p < 0.05) in the 6% DMA group. The antioxidant enzyme activity and lipid peroxidation were negatively (p < 0.05) affected by the increase in DMA concentration. Furthermore, some anti-freeze-associated genes such as heat shock protein 70 (HSP70) and ras homolog family member A (RHOA) were linearly and quadratically down-regulated (p < 0.05) with the high concentration of DMA. Finally, the fertility and hatchability rates did not indicate statistical differences between DMA groups. It can be concluded that using the low concentration of 3–6% DMA in the freezing semen extender is preferable to obtain acceptable results in the post-thawed sperm quality and fertility.

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From Germ Cell Preservation to Regenerative Medicine: An Exciting Research Career in Biotechnology

Abstract: Collection, manipulation, assessment, and storage of mammalian gametes, embryos, and stem cells are providing important opportunities in agriculture, research, and medicine. Semen and embryo freezing in livestock are used in breeding schemes,

Cited by 2 publications

References 59 publications

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm

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