From genome to function: the Arabidopsis aquaporins

Quigley, Françoise; Rosenberg, Joshua M.; Shachar‐Hill, Yair; Bohnert, Hans J.

doi:10.1186/gb-2001-3-1-research0001

Cited by 183 publications

(62 citation statements)

References 90 publications

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“…The two amplicons observed in Aquaporin gene suggest the presence of isoforms as was observed in other plants, like aquaporins in Arabidopsis thaliana (Quigley et al 2002;Javot et al 2003), Zea mays (Maurel et al 2008) and Oryza sativa (Sakurai et al 2005). These AcAQP fragments could also correspond to pseudogenes.…”

Section: Discussionsupporting

confidence: 53%

DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis

Pomponio¹,

Torales²,

Gallo³

et al. 2013

Electron. J. Biotechnol.

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Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.

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Section: Discussionsupporting

confidence: 53%

DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis

Pomponio¹,

Torales²,

Gallo³

et al. 2013

Electron. J. Biotechnol.

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“…The PIPs identified in this study are highly homologous at both the nucleotide and amino acid level. This is characteristic of PIP aquaporins with very high homologies found in other species such as Arabidopsis (Johanson et al 2001;Quigley et al 2002), Brassica (Marin-Olivier et al 2000), walnut (Sakr et al 2003), maize (Chaumont et al 2001), rice (Sakurai et al 2005) and wheat (Forrest and Bhave 2008).…”

Section: Identification and Phylogenetic Analysis Of Grapevine Aquapomentioning

confidence: 86%

“…The MIP superfamily can be divided into four subfamilies; the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-like intrinsic proteins (NIPs) and the small basic intrinsic proteins (SIPs) (Johanson et al 2001;Zardoya and Villalba 2001;Quigley et al 2002;Forrest and Bhave 2008). Two new subfamilies have recently been proposed in the moss Physcomitrella patens (Hedw.)…”

Section: Introductionmentioning

confidence: 99%

Identification and functional characterisation of aquaporins in the grapevine, Vitis vinifera

Shelden

Howitt

Kaiser

et al. 2009

Functional Plant Biol.

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Abstract. Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs).There is limited information about the diversity of MIPs in grapevine, and their water transport capacity. The aim of the present study was to identify MIPs from grapevine and functionally characterise water transport of a subset of MIPs. Candidate genes were identified, by screening a Vitis vinifera L. (cv. Cabernet Sauvignon) cDNA library with gene specific probes, for aquaporin cDNAs encoding members of the plasma membrane intrinsic protein (PIP) and tonoplast intrinsic protein (TIP) subfamilies. The screen resulted in the identification of 11 full-length and two partial length aquaporin cDNAs. VvTIP2;1 isoforms had different 3 0 UTRs, immediately upstream of the poly(A) tail, suggesting the presence of multiple cleavage sites for polyadenylation. Using published genome sequences of grapevine, we conducted a phylogenetic analysis of the MIPs with previously characterised MIPs from Arabidopsis. We identified 23 full-length MIP genes from the V. vinifera genome sequence of a near homozygous line (PN40024) that cluster into the four main subfamilies (and subgroups within) identified in other species. However, based on the identification of PIP2 genes in Cabernet Sauvignon that were not present in the PN40024 genome, there are likely to be more than 23 MIP genes in other heterozygous grapevine cultivars. Water transport capacity was determined for several PIPs and TIPs, by expression in Xenopus oocytes. Only VvPIP2 and VvTIP proteins function as water channels with the exception of VvPIP2;5. VvPIP2;5 differs from the water conducting VvPIP2;1 by the substitution of two highly conserved amino acids in Loop B (G97S, G100W), which was shown by homology modelling to likely form a hydrophobic block of the water pore.

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“…The competition studies for the uptake of As(III) with glycerol in rice led to the suggestion that As(III) is transported by members of the MIP family in plants [56]. Plant MIPs form a large and divergent family, with more than 30 identified members in the genomes of Oryza sativa [57], Arabidopsis thalianab [58], and Zea mays [59]. Based on the sequence similarity and localization, plant MIPs have been classified into four subfamilies [60,61]: plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small and basic intrinsic proteins (SIPs).…”

Section: Aquaglyceroporins (Aqps) and Metalloid Uptakementioning

confidence: 99%