From “Every Day” Hormonal to Oxidative Stress Biomarkers in Blood and Follicular Fluid, to Embryo Quality and Pregnancy Success?

Olszak-Wąsik, Katarzyna; Bednarska-Czerwińska, Anna; Olejek, Anita; Tukiendorf, Andrzej

doi:10.1155/2019/1092415

Cited by 11 publications

(6 citation statements)

References 43 publications

(41 reference statements)

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“…Logistic regression analysis revealed that the antioxidant activity of follicular fluid is a good predictor of oocyte fertilization and top-quality embryos. Both TOSC vs. peroxyl radicals and TOSC vs. hydroxyl radicals demonstrated high sensitivity and specificity, accordingly to previous published data [ 29 ]. Similarly, IL-10 displayed a good predictive ability for fertilization and embryo quality, thus revealing the importance of maintaining an oocyte microenvironment with a specific anti-inflammatory status.…”

Section: Discussionsupporting

confidence: 88%

Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality

Artini

Scarfò

Marzi

et al. 2022

IJMS

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Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential.

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Section: Discussionsupporting

confidence: 88%

Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality

Artini

Scarfò

Marzi

et al. 2022

IJMS

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“…Our study confirmed that the ovarian GCs and FF of patients with repeated COS (placebo group) did show higher levels of 8-OHdG. Moreover, high levels of SOD and GSH-Px activity were related to the success rate of IVF 42 , 43 , which suggested that poor clinical pregnancy outcomes and mitochondrial function damage were associated with the imbalance of oxidation and antioxidation caused by repeated COS. However, administration of BSTJF in our study significantly decreased the levels of 8-OHdG but increased the Mn-SOD and GSH-Px levels compared to the placebo group, indicating that BSTJF can improve mitochondrial function and clinical pregnancy outcomes by adjusting the oxidation-antioxidation balance.…”

Section: Discussionsupporting

confidence: 81%

Effects of Bushen-Tiaojing-Fang on the pregnancy outcomes of infertile patients with repeated controlled ovarian stimulation

Hao

Zhao

et al. 2021

Sci Rep

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Bushen-Tiaojing-Fang (BSTJF) is commonly used to treat infertility. This study investigated the effects of BSTJF on the pregnancy outcomes of patients with repeated controlled ovarian stimulation (COS), on mitochondrial function, and on oxidative stress in ovarian granulosa cells (GCs) and follicular fluid (FF). The samples and clinical data of 97 patients, including 35 in the control group, 29 in the placebo group and 33 in the BSTJF group, were collected for this study. The mitochondrial ultrastructure, ATP content, mitochondrial DNA (mtDNA) number, 8-hydroxy-2-deoxyguanosine (8-OHdG), Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GSH-Px) activity levels, and mRNA expression levels of Mn-SOD, GSH-Px, and nuclear factor erythroid-derived factor 2-related factor 2 (Nrf2) were analyzed. The high-grade embryo (P < 0.001), implantation (P = 0.033), and clinical pregnancy (P = 0.031) rates, as well as the ATP content (P = 0.014), mtDNA number (P = 0.035), GSH-Px activity (P = 0.004 in GCs and P = 0.008 in FF) and mRNA expression levels (P = 0.019), were significantly lower in the placebo group than in the control group, whereas the 8-OHdG content was significantly (P = 0.006 in FF) higher in the placebo group than in the control group. Compared with those in the placebo group, the high-grade embryo rate (P = 0.007), antioxidant enzyme activity (P = 0.037 and 0.036 in Mn-SOD; P = 0.047 and 0.030 in GSH-Px) and mRNA level (P < 0.001 in Nrf2, P = 0.039 in Mn-SOD and P = 0.002 in GSH-Px) were significantly higher in the BSTJF group, as were changes in mitochondrial ultrastructure, ATP (P = 0.040) and mtDNA number (P = 0.013). In conclusion, BSTJF can improve oxidative stress in patients with repeated COS and pregnancy outcomes.

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“…When assessing hydrogen peroxide in FF of the sibling follicles, the highest concentrations are observed from follicles following poor embryo quality, and the lowest concentrations are found in empty follicles [ 34 ]. Women with idiopathic infertility and greater GPx activity are related to high embryo quality [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…Nitrate overproduction is attributed to women who did not achieve pregnancy [ 36 ]. Women who achieved pregnancy presented lower ROS, MDA, protein carbonyls, GR, SOD, and GST and higher TAC, GPX, and tocopherol [ 24 , 35 , 37 – 41 ]. Thus, enhanced lipid and protein oxidation are present among women with an unfavourable outcome.…”

Section: Resultsmentioning

confidence: 99%

Oxidative homeostasis in follicular fluid and reproductive outcomes – from bench to bedside

Zec¹,

Goldštajn²,

Kuna³

et al. 2022

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Free radicals and oxidant molecules are part of our organism in a stable balance. However, when addressing female infertility, questions about their role in oocyte quality arise. This review outlines the major alterations of redox homeostasis in the follicular fluid through pathophysiological conditions in female reproduction and its potential effect on IVF outcome. A review of the literature was accurately performed. Manuscripts investigating follicular fluid biomarkers, especially related to oxidant molecules, were screened and used in this review. Studies assessing the follicular reactive species were found and screened. Moreover, studies assessing the IVF outcomes related to biomarkers were considered. The results are provided in an analytical pathway. The study of biomarkers confirms the shift to enhanced oxidizing modification of macromolecules and antioxidative consumption in the follicular fluid of women undergoing IVF treatment. A lack of congruency in methods appears to be marked in the design of scientific studies. However, it is not clear whether redox disbalance has a disruptive effect on the oocyte competence or whether it plays a role in the oocyte maturation process. Red-ox balance plays a questionable role in IVF outcomes. Possible further insights may consider the antioxidant role of adjuvants during controlled ovarian stimulation cycles.

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From “Every Day” Hormonal to Oxidative Stress Biomarkers in Blood and Follicular Fluid, to Embryo Quality and Pregnancy Success?

Cited by 11 publications

References 43 publications

Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality

Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality

Effects of Bushen-Tiaojing-Fang on the pregnancy outcomes of infertile patients with repeated controlled ovarian stimulation

Oxidative homeostasis in follicular fluid and reproductive outcomes – from bench to bedside

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