From Endogenous to Synthetic microRNA-Mediated Regulatory Circuits: An Overview

Ferro, Elsi; Bena, Chiara Enrico; Grigolon, Silvia; Bosia, Carla

doi:10.3390/cells8121540

Cited by 20 publications

(16 citation statements)

References 189 publications

(341 reference statements)

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“…Noncoding short microRNAs (miRNA; 18-25 nucleotides) are present, both intra-and extracellularly, in different body fluids of humans and animals (Ferro et al, 2019;Matsuyama and Suzuki, 2019). They can have very different biological functions, including regulation of genes (Miller and Wahlestedt, 2010;Melnik and Schmitz, 2017).…”

Section: Introductionmentioning

confidence: 99%

Secretory immunoglobulin A from human milk hydrolyzes microRNA

Kompaneets

Ermakov

Sedykh

et al. 2020

Journal of Dairy Science

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For breast-fed infants, human milk is a source of various nutrients (e.g., proteins, peptides, antibodies) and bioactive components that promote neonatal growth and protect infants from viral and bacterial infection. Moreover, in terms of infant nutrition and protection the functions of many human milk components are very different from those of blood and other biological fluids of healthy adults. For example, catalytic antibodies ("abzymes") with synthetic activities (protein, oligosaccharide, and lipid kinase activities) have been found in human breast milk that are absent in the blood of healthy people. Abzymes with hydrolyzing functions have been detected not only in milk, but also in the blood of patients with autoimmune diseases. Obviously, feeding newborns human milk has a very specific role and it is a unique aspect of mammalian nutrition. Ribonuclease and DNase autoantibodies or abzymes are found in milk and blood of lactating women, but not in blood sera of healthy men and nonpregnant woman. Here, we present the first evidence that human milk secretory IgA molecules (sIgA) can effectively hydrolyze ribooligonucleotides containing 23 different bases [(pN) 23 ribooligonucleotides] and 4 microRNAs: miR-9-5p, miR-219-2-3p, miR-137, and miR-219a-5p. Ribonuclease activity is an inherent property of sIgAs. We showed that 7 individual sIgAs hydrolyzed the ribooligonucleotides (pA) 23 , (pU) 23 , and (pC) 23 nonspecifically and with comparable efficiency, whereas hydrolysis of the 4 microRNAs by sIgAs was site-specific. Sites of hydrolysis of 4 microRNAs by IgG from blood of patients with schizophrenia have been previously identified. The sites of hydrolysis of 4 microRNAs by sIgA-abzymes were very different from the previously identified sites of hydrolysis by IgG in patients with schizophrenia. In addition, in contrast to IgG, milk sIgAs efficiently hydrolyzed microRNAs in their loop and duplex regions.

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Section: Introductionmentioning

confidence: 99%

Secretory immunoglobulin A from human milk hydrolyzes microRNA

Kompaneets

Ermakov

Sedykh

et al. 2020

Journal of Dairy Science

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“…miRNA mediated regulation could serve to manage the impact of noise in gene expression. 91 A number of studies have addressed the relationship between miRNA and noise, and the results suggested that miRNAs could provide precision to protein expression by buffering noise when needed or exploit stochasticity in biological contexts that take advantage of gene expression variability, such as differentiation processes. 91 The coherent, quite uniform variations of the miRNAs in our work appear to be in line with this.…”

Section: Discussionmentioning

confidence: 99%

Rapid changes of miRNAs-20, -30, −410, −515, −134, and −183 and telomerase with psychological activity: A one year study on the relaxation response and epistemological considerations

Lin

Marinova

Brugnolo

et al. 2021

Journal of Traditional and Complementary Medicine

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Background and aim Mental stress represents a pivotal factor in cardiovascular diseases. The mechanism by which stress produces its deleterious effects is still under study, but one of the most explored pathways is inflammation-aging and cell senescence. In this scenario, circulating microRNAs appear to be regulatory elements of the telomerase activity and alternative splicing within the nuclear factor kappa-light-chain-enhancer (NF-κB) network. Anti-stress techniques appeared to be able to slow down the inflammatory and aging processes. As we recently verified, the practice of the relaxation response (RR) counteracted psychological stress and determined favorable changes of the NF-κB, p53, and toll-like receptor-4 (TLR-4) gene expression and in neurotransmitters, hormones, cytokines, and inflammatory circulating microRNAs. We aimed to verify a possible change in the serum levels of six other micro-RNAs of cardiovascular interest, involved in cell senescence and in the NF-κB network (miRNAs −20, −30, −410, −515, −134, and −183), and tested the activity of telomerase in peripheral blood mononuclear cells (PBMCs). Experimental procedure We measured the aforementioned molecules in the serum of patients with ischemic heart disease (and healthy controls) immediately before and after a relaxation response session, three times (after the baseline), in one year of follow-up. Results According to our data, the miRNA-20 and -30 levels and PBMCs-telomerase activity increased during the RR while the −410 and −515 levels decreased. During the RR sessions, both miRNA-134 and -183 decreased. Conclusion The mediators considered in this exploratory work appeared to vary rapidly with the psychological activity (in particular when focused on relaxation techniques) showing that psychological activity should be part of the future research on epigenetics. Epistemological perspectives are also discussed.

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“…Noncoding short microRNAs (miRNA) are both intra-and extra-cellular and are found in different body fluids of mammals [53,54]. They regulate up to several hundred genes and have many other, different biological functions [55,56].…”

Section: Introductionmentioning

confidence: 99%

IgGs from Human Milk Hydrolyze microRNAs

et al. 2020

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Mother’s milk provides breast-fed infants with various nutrients, including peptides, proteins, DNA, RNA, antibodies, and other bioactive components promoting neonatal growth and protecting infants from viral and bacterial infection. The functions of many human milk components regarding the nutrition and protection of newborns may be very different compared to those of various biological fluids of healthy adults. For example, human milk contains catalytic antibodies (abzymes) with protein, lipid, and oligosaccharide kinase activities, which are absent in the biological fluids of healthy people and autoimmune patients. Obviously, the nutrition of infants with fresh breast milk is a special phenomenon having a very specific and important role. Here, we have shown that mother’s milk IgGs effectively split homo-(pN)23, and four miRNAs: miR-137, miR-219a-5p, miR-219-2-3p, and miR-9-5p. It was shown that ribonuclease activity is a unique property of milk IgGs. On average, individual IgGs hydrolyze (pA)23, (pU)23, and (pC)23 nonspecifically and with comparable efficiency, whereas the hydrolysis of four miRNAs is predominately site-specific. The specific sites of the hydrolysis of four miRNAs by IgGs from the blood of schizophrenic (SCZ) patients and secretory immunoglobulins A (sIgAs) from human milk were found earlier. The sites of the hydrolysis of four miRNAs by milk IgGs and sIgA-abzymes are almost the same, but are significantly different in comparison with those for SCZ IgGs. In addition, in contrast to the SCZ IgGs, milk IgGs and sIgAs efficiently hydrolyzed miRNAs in the duplex regions formed by their terminal sequences.

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From Endogenous to Synthetic microRNA-Mediated Regulatory Circuits: An Overview

Cited by 20 publications

References 189 publications

Secretory immunoglobulin A from human milk hydrolyzes microRNA

Secretory immunoglobulin A from human milk hydrolyzes microRNA

Rapid changes of miRNAs-20, -30, −410, −515, −134, and −183 and telomerase with psychological activity: A one year study on the relaxation response and epistemological considerations

IgGs from Human Milk Hydrolyze microRNAs

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