From early homologue recognition to synaptonemal complex formation

Zickler, Denise

doi:10.1007/s00412-006-0048-6

Cited by 211 publications

(239 citation statements)

References 178 publications

(230 reference statements)

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“…Bouquet formation is a key event of a general repositioning of chromosomes and is thought to facilitate homologous chromosome pairing and synapsis (Harper et al, 2004;Zickler, 2006). To examine bouquet formation, we conducted fluorescence in situ hybridization (FISH) analysis using pAtT4 con- OsRec8 encodes a rice OsRad21-4 protein.…”

Section: Osrec8 Mutants Show Defective Telomere Bouquet Formationmentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

119

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The successful transmission of chromosomes during mitosis and meiosis relies on the establishment and subsequent release of cohesion between replicated chromatids. Cohesion is mediated by a four-subunit structural maintenance of chromosome complex, called cohesins. REC8 is a key component of this meiotic cohesion complex in most model organisms studied to date. Here, we isolated and dissected the functions of OsREC8, a rice (Oryza sativa) REC8 homolog, using two null Osrec8 mutants. We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes. Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I. Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8. By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins. These results suggest that OsREC8 has several essential roles in the meiotic processes.

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Section: Osrec8 Mutants Show Defective Telomere Bouquet Formationmentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

119

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“…The AT5+ motifs we have described could play such a role. In preleptotene, DNA synthesis has already been finished and sister chromatids remain paired, chromosomes search their homologues [25]. The process usually starts in specific regions which contain repeated sequences, such as 9 centromeres or telomeres.…”

Section: Homologue Chromosome Recognition In Meiosismentioning

confidence: 99%

The distribution of alternating AT sequences in eukaryotic genomes suggests a role in homologous chromosome recognition in meiosis

Subirana

Messeguer

2011

Journal of Theoretical Biology

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There are general features of chromosome dynamics, such as homologue recognition in early meiosis, which are expected to involve related sequence motifs in non-coding DNA, with a similar distribution in different species. A search for such motifs is presented here. It has been carried out with the CONREPP program. It has been found that short alternating AT sequences (10-20 bases) have a similar distribution in most eukaryotic organisms, with some exceptions related to unique meiotic features. All other microsatellite and repeat sequences vary significantly in different organisms. It is concluded that the unique structural features and uniform distribution of alternating AT sequences indicate that they may facilitate homologous chromosome pairing in the early preleptotene stage of meiosis. They may also play a role in the compaction of DNA in mitotic chromosomes.

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“…Indeed, there is evidence to suggest that before strand breakage occurs, identical intact double-stranded DNA segments may pair. 1,[9][10][11][12] It is also conceivable that homology recognition between intact DNA tracts may occur in other biological processes. One instance might be the silencing of multiple copies of the same gene.…”

Section: Introductionmentioning

confidence: 99%

Which way up? Recognition of homologous DNA segments in parallel and antiparallel alignments

Lee

Wynveen

Albrecht

et al. 2015

The Journal of Chemical Physics

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Homologous gene shuffling between DNA molecules promotes genetic diversity and is an important pathway for DNA repair. For this to occur, homologous genes need to find and recognize each other. However, despite its central role in homologous recombination, the mechanism of homology recognition has remained an unsolved puzzle of molecular biology. While specific proteins are known to play a role at later stages of recombination, an initial coarse grained recognition step has, however, been proposed. This relies on the sequence dependence of the DNA structural parameters, such as twist and rise, mediated by intermolecular interactions, in particular, electrostatic ones. In this proposed mechanism, sequences that have the same base pair text, or are homologous, have lower interaction energy than those sequences with uncorrelated base pair texts. The difference between the two energies is termed the "recognition energy." Here, we probe how the recognition energy changes when one DNA fragment slides past another, and consider, for the first time, homologous sequences in antiparallel alignment. This dependence on sliding is termed the "recognition well." We find there is a recognition well for anti-parallel, homologous DNA tracts, but only a very shallow one, so that their interaction will differ little from the interaction between two nonhomologous tracts. This fact may be utilized in single molecule experiments specially targeted to test the theory. As well as this, we test previous theoretical approximations in calculating the recognition well for parallel molecules against MC simulations and consider more rigorously the optimization of the orientations of the fragments about their long axes upon calculating these recognition energies. The more rigorous treatment affects the recognition energy a little, when the molecules are considered rigid. When torsional flexibility of the DNA molecules is introduced, we find excellent agreement between the analytical approximation and simulations. C 2015 AIP Publishing LLC. [http://dx

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From early homologue recognition to synaptonemal complex formation

Cited by 211 publications

References 178 publications

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

The distribution of alternating AT sequences in eukaryotic genomes suggests a role in homologous chromosome recognition in meiosis

Which way up? Recognition of homologous DNA segments in parallel and antiparallel alignments

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