From climate change to molecular response: redox proteomics of ozone‐induced responses in soybean

Galant, Ashley; Koester, Robert P.; Ainsworth, Elizabeth A.; Hicks, Leslie M.; Jez, Joseph M.

doi:10.1111/j.1469-8137.2011.04037.x

Cited by 58 publications

(30 citation statements)

References 62 publications

(236 reference statements)

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“…A recent proteomics experiment investigating the timing of changes in thylakoid proteins to O 3 exposure showed decreased abundance of most proteins within 14 d of exposure to O 3 (Bohler et al, 2011), consistent with the transcriptional changes reported here. Previous work at this site also demonstrated that oxidation of Rubisco protein was significantly greater in plants exposed to elevated [O 3 ] (Galant et al, 2012). In this study, the decrease in photosynthetic metabolism fed forward to alter leaf contents of sugars, sugar alcohols, and starch.…”

Section: O 3 Decreases Metabolites Associated With Primary Metabolismsupporting

confidence: 49%

Ozone Exposure Response for U.S. Soybean Cultivars: Linear Reductions in Photosynthetic Potential, Biomass, and Yield

et al. 2012

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Current background ozone (O 3 ) concentrations over the northern hemisphere's midlatitudes are high enough to damage crops and are projected to increase. Soybean (Glycine max) is particularly sensitive to O 3 ; therefore, establishing an O 3 exposure threshold for damage is critical to understanding the current and future impact of this pollutant. This study aims to determine the exposure response of soybean to elevated tropospheric O 3 by measuring the agronomic, biochemical, and physiological responses of seven soybean genotypes to nine O 3 concentrations (38-120 nL L 21 ) within a fully open-air agricultural field location across 2 years. All genotypes responded similarly, with season-long exposure to O 3 causing a linear increase in antioxidant capacity while reducing leaf area, light absorption, specific leaf mass, primary metabolites, seed yield, and harvest index. Across two seasons with different temperature and rainfall patterns, there was a robust linear yield decrease of 37 to 39 kg ha 21 per nL L 21 cumulative O 3 exposure over 40 nL L 21 . The existence of immediate effects of O 3 on photosynthesis, stomatal conductance, and photosynthetic transcript abundance before and after the initiation and termination of O 3 fumigation were concurrently assessed, and there was no evidence to support an instantaneous photosynthetic response. The ability of the soybean canopy to intercept radiation, the efficiency of photosynthesis, and the harvest index were all negatively impacted by O 3 , suggesting that there are multiple targets for improving soybean responses to this damaging air pollutant.

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Section: O 3 Decreases Metabolites Associated With Primary Metabolismsupporting

confidence: 49%

Ozone Exposure Response for U.S. Soybean Cultivars: Linear Reductions in Photosynthetic Potential, Biomass, and Yield

et al. 2012

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“…To date, no information is available regarding ozone-induced changes in the S-nitroso-proteome of plants. Although several studies describe the impact of acute ozone exposure on total proteomes of rice, soybean, wheat, and poplar [29]–[32], the issue of redox-linked protein modifications upon ozone has only been examined in two studies [33], [34]. Ozone exerts bi-functional effects on earth.…”

Section: Introductionmentioning

confidence: 99%

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

et al. 2014

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Protein S-nitrosylation, the covalent binding of nitric oxide (NO) to protein cysteine residues, is one of the main mechanisms of NO signaling in plant and animal cells. Using a combination of the biotin switch assay and label-free LC-MS/MS analysis, we revealed the S-nitroso-proteome of the woody model plant Populus x canescens. Under normal conditions, constitutively S-nitrosylated proteins in poplar leaves and calli comprise all aspects of primary and secondary metabolism. Acute ozone fumigation was applied to elicit ROS-mediated changes of the S-nitroso-proteome. This treatment changed the total nitrite and nitrosothiol contents of poplar leaves and affected the homeostasis of 32 S-nitrosylated proteins. Multivariate data analysis revealed that ozone exposure negatively affected the S-nitrosylation status of leaf proteins: 23 proteins were de-nitrosylated and 9 proteins had increased S-nitrosylation content compared to the control. Phenylalanine ammonia-lyase 2 (log2[ozone/control] = −3.6) and caffeic acid O-methyltransferase (−3.4), key enzymes catalyzing important steps in the phenylpropanoid and subsequent lignin biosynthetic pathways, respectively, were de-nitrosylated upon ozone stress. Measuring the in vivo and in vitro phenylalanine ammonia-lyase activity indicated that the increase of the phenylalanine ammonia-lyase activity in response to acute ozone is partly regulated by de-nitrosylation, which might favor a higher metabolic flux through the phenylpropanoid pathway within minutes after ozone exposure.

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“…For instance, it is estimated that by 2020 current day, the yield losses due to O 3 damage will rise from current 13% and 23%, to 16% and 35%, respectively for cereals and soybean productions of China [3], [4]. Economically, annual crop losses induced by O 3 are estimated at $3–5.5 billion in China, and will likely increase by 16%–110% in the near future [5], [6]. These predictions suggest that China in particular might be on the cusp of substantial reductions in grain production following the rapid economic transformation, industrialization and urbanization [7].…”

Section: Introductionmentioning

confidence: 99%

Flixweed Is More Competitive than Winter Wheat under Ozone Pollution: Evidences from Membrane Lipid Peroxidation, Antioxidant Enzymes and Biomass

Wang

et al. 2013

PLoS ONE

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To investigate the effects of ozone on winter wheat and flixweed under competition, two species were exposed to ambient, elevated and high [O3] for 30 days, planted singly or in mixculture. Eco-physiological responses were examined at different [O3] and fumigating time. Ozone reduced the contents of chlorophyll, increased the accumulation of H2O2 and malondialdehyde in both wheat and flixweed. The effects of competition on chlorophyll content of wheat emerged at elevated and high [O3], while that of flixweed emerged only at high [O3]. The increase of H2O2 and malondialdehyde of flixweed was less than that of wheat under the same condition. Antioxidant enzyme activities of wheat and flixweed were seriously depressed by perennial and serious treatment using O3. However, short-term and moderate fumigation increased the activities of SOD and POD of wheat, and CAT of flixweed. The expression levels of antioxidant enzymes related genes provided explanation for these results. Furthermore, the increase of CAT expression of flixweed was much higher than that of SOD and POD expression of wheat. Ozone and competition resulted in significant reductions in biomass and grain yield in both winter wheat and flixweed. However, the negative effects on flixweed were less than wheat. Our results demonstrated that winter wheat is more sensitive to O3 and competition than flixweed, providing valuable data for further investigation on responses of winter wheat to ozone pollution, in particular combined with species competition.

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From climate change to molecular response: redox proteomics of ozone‐induced responses in soybean

Cited by 58 publications

References 62 publications

Ozone Exposure Response for U.S. Soybean Cultivars: Linear Reductions in Photosynthetic Potential, Biomass, and Yield

Ozone Exposure Response for U.S. Soybean Cultivars: Linear Reductions in Photosynthetic Potential, Biomass, and Yield

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

Flixweed Is More Competitive than Winter Wheat under Ozone Pollution: Evidences from Membrane Lipid Peroxidation, Antioxidant Enzymes and Biomass

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