From Chronic Feed-Induced Intestinal Inflammation to Adenocarcinoma with Metastases in Salmonid Fish

Dale, Ole Bendik; Tørud, Brit; Kvellestad, Agnar; Koppang, Hanna Strömme; Koppang, Erling O.

doi:10.1158/0008-5472.can-08-4877

Cited by 50 publications

(41 citation statements)

References 38 publications

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“…30 Diet as a cause of the intestinal lesions described here was unlikely as a potential source of carcinogenesis based upon the experiment carried out at SARL. Data from the retrospective study suggest that intestinal lesions may be observed as early as 6 months of age and the sampling at 6 mo yielded no sign of preneoplastic or neoplastic intestinal changes.…”

Section: Discussionmentioning

confidence: 83%

A Retrospective Study of the Prevalence and Classification of Intestinal Neoplasia in Zebrafish (Danio Rerio)

et al. 2013

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For over a decade, spontaneous intestinal neoplasia has been observed in zebrafish (Danio rerio) submitted to the ZIRC (Zebrafish International Resource Center) diagnostic service. In addition, zebrafish displayed preneoplastic intestinal changes including hyperplasia, dysplasia, and enteritis. A total of 195 zebrafish, representing 2% of the total fish submitted to the service, were diagnosed with these lesions. Neoplastic changes were classified either as adenocarcinoma or small cell carcinoma, with a few exceptions (carcinoma not otherwise specified, tubular adenoma, and tubulovillous adenoma). Tumor prevalence appeared similarly distributed between sexes and generally occurred in zebrafish greater than 1 year of age, although neoplastic changes were observed in fish 6 months of age. Eleven lines displayed these preneoplastic and neoplastic changes, including wild-types and mutants. Affected zebrafish originated from 18 facilities, but the majority of fish were from a single zebrafish research facility (hereafter referred to as the primary facility) that has submitted numerous samples to the ZIRC diagnostic service. Zebrafish from the primary facility submitted as normal sentinel fish demonstrate that these lesions are most often subclinical. Fish fed the diet from the primary facility and held at another location did not develop intestinal lesions, indicating that diet is not the etiologic agent.

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Section: Discussionmentioning

confidence: 83%

A Retrospective Study of the Prevalence and Classification of Intestinal Neoplasia in Zebrafish (Danio Rerio)

et al. 2013

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“…Because of the lack of reliable mesenchymal markers in koi carp tissue, application was restricted to these epithelial markers. CK AE1/AE 3 clearly identified intestinal epithelial cells, also shown before (Dale et al 2009); however, in the gonad and in the liver, staining was inconclusive. This staining pattern was confirmed by our results in the reference carp tissue, with hepatocytes staining negative and inconsistent staining in ovary epithelial cells.…”

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confidence: 64%

“…First, a broad-spectrum antibody recognising most of the 19 human cyto keratins (Cytokeratin [CK] AE1/AE3, Dako; dilution 1:50) was used. Specificity is described for a number of fish species, including striped bass Morone saxa tilis and medaka Oryzias latipes (Bunton 1993) or Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss (Dale et al 2009). To test the re activity in carp tissue, normal carp liver, intestine and gonads (ovary and testis) were tested and a score was formulated according to low, moderate or strong signals obtained (Table 1, Fig.…”

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confidence: 99%

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Frequency and histologic characterization of coelomatic neoplasms in koi Cyprinus carpio koi

Knüsel¹,

Knüsel²,

Doherr³

et al. 2016

Dis. Aquat. Org.

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Koi carp presenting with coelomatic neoplastic lesions are of growing importance in Switzerland and elsewhere in Europe, as clinical diagnosis is usually difficult and prognosis is usually poor because of late recognition. To gain more information about this condition, between 2008 and 2012, 117 koi diagnosed with coelomatic tumours were included in this study. The nature and growth characteristics of these pathologic tissues were documented. Out of 117 koi, 48.9% (n = 57) were female; the ovary was the most often affected organ (35%, n = 41), and in 62.4% (n =73) of cases, the cells of origin were identified as stromal cells of the sex-cord and the tumours were identified as sex-cord stromal tumours. The characteristics of growth and histology showed that the majority of tumours were malignant.KEY WORDS: Koi carp · Neoplasm · Histopathology · Ovary · Testis · Sex-cord stromal tumour Resale or republication not permitted without written consent of the publisherDis Aquat Org 119: [219][220][221][222][223][224][225][226][227][228][229] 2016 To gain more information about the nature of neoplastic lesions in internal organs and the pathological features of these neoplasms, koi presenting with various abdominal neoplasms were examined between 2008 and 2012. Specifically, possible sex-specific differences, the organs affected, the tissue/cells of origin and the characteristics of neoplastic growth were investigated. MATERIALS AND METHODS Origin of samplesBetween 2008 and 2012, all koi carp with an enlarged abdomen, which were euthanized or died in our veterinary clinic, were examined pathologically. A standard necropsy was performed. Those fish showing a coelomatic mass suspected to be a tumour were included in this study, for a total of 117 animals. As there was no recurrence of tumour masses in the same animal, 1 tumour case always corresponds to 1 animal. If possible, the sex of the affected animal was recorded. Macroscopic changes of internal organs, including extent and appearance of the coelomatic mass (colour, surface, cut section and texture) were recorded in 62 cases. In all 117 cases, tissue samples of different representative areas were fixed and stored in 10% buffered formalin and sent for analysis to the Centre for Fish and Wildlife Health (University of Bern). These samples were identified histologically as coelomatic neoplastic lesions. This criterion was used for the final decision to include the animals in this study. Histopathology and immunohistochemistry (IHC)Formalin-fixed samples were paraffin-embedded and routinely processed. Three or 4 serial sections were prepared: the first sections were stained with haematoxylin-eosin (H&E), the remaining sections were used for IHC staining.As a basis to classify the neoplasms, criteria defined in the World Health Organization classifications for tumours in domestic animals (Kennedy et al. 1998, Head et al. 2003, Meuten et al. 2007) were used.Because of limited availability of antibodies working properly on our koi carp tissue, only an epithelia...

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“…Immunohistochemistry has recently found its application in phenotyping fish neoplasms, such as melanoma, schwannoma, or adenocarcinoma, through expression of markers such as S100 protein, calretinin, or cytokeratin (Sakamoto & White 2002, Marino et al 2007, Magi et al 2008, Marino et al 2008, Dale et al 2009). The use of mammalian antibodies in fish tissues has some limitations related to their specificity.…”

Section: Discussionmentioning

confidence: 99%

Seminoma in a koi carp Cyprinus carpio: histopathological and immunohistochemical findings

Sirri¹,

Mandrioli²,

Grieco³

et al. 2010

Dis. Aquat. Org.

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A spontaneous seminoma in a 3 yr old male koi carp Cyprinus carpio L. is described. The animal, presenting a symmetric abdominal enlargement, showed a celomatic multinodular, whiteyellowish and firm mass that infiltrated the liver and the intestine wall. Histologically, the neoplasm was non-encapsulated and poorly demarcated, showed invasive growth and was characterized by a lobular architecture, subdivided by abundant fibro-connective septa. Large necrotic and calcified areas together with small aggregates of residual spermatids were present. We diagnosed a classical seminoma with a diffuse pattern. Neoplastic cells cross-reacted with vimentin, placental alkaline phosphatase, and c-KIT. An immunohistochemical phenotypization of the tumor was performed to exclude other celomatic neoplasms and to compare this seminoma with those reported in mammals and humans. KEY WORDS: Seminoma · Koi carp · Histology · Immunohistochemistry Resale or republication not permitted without written consent of the publisherDis Aquat Org 92: [83][84][85][86][87][88] 2010 The tumor is described histopathologically, and an immunohistochemical phenotypization was performed to exclude other celomatic neoplasms such as hepatic or intestinal carcinoma and lymphoma and to study similarities with mammalian and human seminoma. MATERIALS AND METHODSA 3 yr old male koi carp Cyprinus carpio L. with gross pathological symptoms consisting of a symmetrical abdominal enlargement was examined histologically and immunohistochemically. The first 2 yr, the fish was kept in a farm with natural reproduction in Italy. It was then kept for 1 yr with other koi carp in a pet shop tank, during which period it was not treated with chemicals.Immediately after death, the animal was sent to the Servizio di Anatomia Patologica, Deparment of Veterinary Public Health and Animal Pathology, Faculty of Veterinary, Bologna, Italy, for further investigation. A complete necropsy was performed, and parts of the neoplastic mass and liver, intestine, swim bladder, kidney, gills, skin, and muscle were removed and fixed in 10% formalin or Bouin's fixative for histological examination. Sections were stained with hematoxylin and eosin (H&E). Additional samples of the mass were stained with periodic acid-Schiff (PAS) to detect the presence of glycogen in gonocytes. For immunohistochemistry, only formalin-fixed tissue samples from the mass were used. Immunohistochemistry was performed with antibodies against cytokeratin (1:50, clone AE1/AE3; Dako), vimentin (prediluted, clone V9; Novocastra), c-KIT (1:600, polyclonal; Dako), placental alkaline phosphatase (PLAP) (1:25, clone 8A9; Dako), alpha-fetoprotein (AFP) (prediluted, clone C3; BioGenex Laboratories), estrogen receptor (ER) (prediluted, clone SP1; Ventana Medical Systems), neuronspecific enolase (NSE) (1:1600, clone AB-1; NeoMarkers), calretinin (1:100, clone AB-1; NeoMarkers), CD3 (1:75, clone F7.2.38; Dako), CD79α (1:10, clone HM57; Dako), CD45RA (1:20, clone 4KB5; Dako). A mammal positive control and a non-diseas...

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From Chronic Feed-Induced Intestinal Inflammation to Adenocarcinoma with Metastases in Salmonid Fish

Abstract: Neoplasms in fish normally show poor abilities for metastasis, and there are no reports on intestinal cancer with metastasis to other organs.

Cited by 50 publications

References 38 publications

A Retrospective Study of the Prevalence and Classification of Intestinal Neoplasia in Zebrafish (Danio Rerio)

A Retrospective Study of the Prevalence and Classification of Intestinal Neoplasia in Zebrafish (Danio Rerio)

Frequency and histologic characterization of coelomatic neoplasms in koi Cyprinus carpio koi

Seminoma in a koi carp Cyprinus carpio: histopathological and immunohistochemical findings

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