From chip-in-a-lab to lab-on-a-chip: towards a single handheld electronic system for multiple application-specific lab-on-a-chip (ASLOC)

Neužil, Pavel; Campos, C.; Wong, Chee Chung; Soon, Jeffrey Bo Woon; Reboud, Julien; Manz, Andreas

doi:10.1039/c4lc00310a

Cited by 55 publications

(34 citation statements)

References 32 publications

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“…Endpoint detection requires less complex instrumentation and provides simpler outputs for interpretation. A similar platform was also developed as a modular system (Neuzil et al, 2014) capable to perform both time and space domain PCR for NA, localized surface plasmon resonance for protein detection (Neuzil and Reboud, 2008), electrochemical sensing using an array of two electrode systems and also using nanowires as biosensors. Alternative methods are developed for NA amplification detection, such as optical, mechanical, chemical, electrochemical, and nanomaterialsbased detection.…”

Section: Detection Methods For Locmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

189

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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Section: Detection Methods For Locmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

189

146

View full text Add to dashboard Cite

show abstract

“…In the past two decades microfluidic and lab-on-a-chip technologies have shown significant promise in a wide range of applications such as: analytical [1][2][3][4] and diagnostic devices, [5][6][7][8] platforms for chemical and material synthesis [9][10][11][12][13] and microphysiological systems. [14][15][16][17][18][19][20] Active flow control and sample delivery remain critical functions in microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

Bubble pump: scalable strategy for in-plane liquid routing

Oskooei

Günther

2015

Lab Chip

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We present an on-chip liquid routing technique intended for application in well-based microfluidic systems that require long-term active pumping at low to medium flowrates. Our technique requires only one fluidic feature layer, one pneumatic control line and does not rely on flexible membranes and mechanical or moving parts. The presented bubble pump is therefore compatible with both elastomeric and rigid substrate materials and the associated scalable manufacturing processes. Directed liquid flow was achieved in a microchannel by an in-series configuration of two previously described "bubble gates", i.e., by gas-bubble enabled miniature gate valves. Only one time-dependent pressure signal is required and initiates at the upstream (active) bubble gate a reciprocating bubble motion. Applied at the downstream (passive) gate a time-constant gas pressure level is applied. In its rest state, the passive gate remains closed and only temporarily opens while the liquid pressure rises due to the active gate's reciprocating bubble motion. We have designed, fabricated and consistently operated our bubble pump with a variety of working liquids for >72 hours. Flow rates of 0-5.5 μl min(-1), were obtained and depended on the selected geometric dimensions, working fluids and actuation frequencies. The maximum operational pressure was 2.9 kPa-9.1 kPa and depended on the interfacial tension of the working fluids. Attainable flow rates compared favorably with those of available micropumps. We achieved flow rate enhancements of 30-100% by operating two bubble pumps in tandem and demonstrated scalability of the concept in a multi-well format with 12 individually and uniformly perfused microchannels (variation in flow rate <7%). We envision the demonstrated concept to allow for the consistent on-chip delivery of a wide range of different liquids that may even include highly reactive or moisture sensitive solutions. The presented bubble pump may provide active flow control for analytical and point-of-care diagnostic devices, as well as for microfluidic cells culture and organ-on-chip platforms.

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“…The platform is fully portable and modular in such a way that several transducers can be exchanged according to the module board incorporated (electrochemical sensors, nanowires, or, for example, Au nanostructures for LSPR sensing; see Figure 2C). The instrumentation required for a real-time measurement is highly integrated, including a microfluidic chamber, a high-voltage source, a temperature controller, and a fourchannel lock-in, all fully controlled by a single chip with a touch-screen LCD display [41]. The LSPR module is based on a previously described integrated device based on LED illumination (four different combined LEDs at different wavelengths) and reflection measurements [42].…”

Section: Breakthroughs In Multiplexing and Loc Platformsmentioning

confidence: 99%

“…A very attractive design that shows a relevant advancement in LOC platform has been the multiple sensor handheld battery-operated platform developed by Neuzil et al [41] (see Figure 2C). The platform is fully portable and modular in such a way that several transducers can be exchanged according to the module board incorporated (electrochemical sensors, nanowires, or, for example, Au nanostructures for LSPR sensing; see Figure 2C).…”

Section: Breakthroughs In Multiplexing and Loc Platformsmentioning

confidence: 99%

Recent advances in nanoplasmonic biosensors: applications and lab-on-a-chip integration

et al. 2016

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Abstract:Motivated by the recent progress in the nanofabrication field and the increasing demand for cost-effective, portable, and easy-to-use point-of-care platforms, localized surface plasmon resonance (LSPR) biosensors have been subjected to a great scientific interest in the last few years. The progress observed in the research of this nanoplasmonic technology is remarkable not only from a nanostructure fabrication point of view but also in the complete development and integration of operative devices and their application. The potential benefits that LSPR biosensors can offer, such as sensor miniaturization, multiplexing opportunities, and enhanced performances, have quickly positioned them as an interesting candidate in the design of lab-on-a-chip (LOC) optical biosensor platforms. This review covers specifically the most significant achievements that occurred in recent years towards the integration of this technology in compact devices, with views of obtaining LOC devices. We also discuss the most relevant examples of the use of the nanoplasmonic biosensors for real bioanalytical and clinical applications from assay development and validation to the identification of the implications, requirements, and challenges to be surpassed to achieve fully operative devices.

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From chip-in-a-lab to lab-on-a-chip: towards a single handheld electronic system for multiple application-specific lab-on-a-chip (ASLOC)

Cited by 55 publications

References 32 publications

Recent advances in lab-on-a-chip technologies for viral diagnosis

Recent advances in lab-on-a-chip technologies for viral diagnosis

Bubble pump: scalable strategy for in-plane liquid routing

Recent advances in nanoplasmonic biosensors: applications and lab-on-a-chip integration

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