From brain to food: Analysis of phosphatidylcholins, lyso-phosphatidylcholins and phosphatidylcholin–plasmalogens derivates in Alzheimer's disease human post mortem brains and mice model via mass spectrometry

Alzheimer's disease–like pathology has transient effects on the brain and blood metabolome

“…It has also been noted that total phosphatidylcholine diacyl (PCaa) levels tend to be lower in APPswe brain (Grimm et al, 2011). However our findings are in broad agreement with a previous study that demonstrated the APP/PS1 mouse brain exhibits increased SPHs and PCs at 9 months (Fabelo et al, 2012).…”

Brain Metabolomics Study on the Protective Effects of Ginsenosides Rg1 and Rb1 in an Alzheimer’s Disease Mouse Model

“…The above showed that Hypoxanthine can be a reliable biomarker for diagnosing AD, while the therapeutically effect of GRg1 a GRb1 did little to purine pathway. In this study, the level of LPCs was obviously decreased in the brain tissue of AD mice compared to the control mice consistent with the previous reports that a lower total LPC concentration was observed in brain, CSF and plasma of AD patients [27][28][29]. LPC is the degradation product of Phosphatidylcholine (PC), which is the major phospholipid of eukaryotic membranes representing approximately 40% of phospholipids in most cellular membranes [30].…”

Simultaneous extraction of metabolome and lipidome with methyl tert-butyl ether from a single small tissue sample for ultra-high performance liquid chromatography/mass spectrometry

“…If coupled with chromatography, the matrix effect from the complex samples is greatly reduced through chromatographic separation [9]. Mass spectrometry-based metabolomic analyses can be employed both to uncover novel metabolites of interest in non-targeted approaches [10,11] and to provide a means of highly sensitive quantification of specific metabolites in targeted settings [12]. Shotgun mass spectrometry [13,14], alone or in combination with liquid chromatography [15,16], is also the predominating platform employed in lipidomic analyses.…”