From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process

Nagel-Heyer, Stephanie; Lünse, Svenja; Leist, C.; Böer, Roman; Goepfert, Christine; Pörtne, Ralf

doi:10.1007/978-1-4020-5476-1_60

Cited by 3 publications

(4 citation statements)

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“…These are considered as a major cause for the dedifferentiation of cells. Recent studies have shown that microcarrier cultures performed in wellmixed bioreactor systems can significantly improve cell expansion (20)(21)(22).…”

Section: Important Aspects For Bioreactor Designmentioning

confidence: 99%

“…In recent studies, small well-mixed bioreactors (e.g., shake flasks, stirred vessels, and super spinner) have been suggested for cell proliferation, in which the cells are grown on microcarriers (20)(21)(22). These systems were used in the cultivation of encapsulated cells (18,19) or neural stem cells in single-cell suspension culture (40).…”

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

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Bioreactor design for tissue engineering

Pörtner

Nagel-Heyer

Goepfert

et al. 2005

Journal of Bioscience and Bioengineering

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275

174

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Section: Important Aspects For Bioreactor Designmentioning

confidence: 99%

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

Bioreactor design for tissue engineering

Pörtner

Nagel-Heyer

Goepfert

et al. 2005

Journal of Bioscience and Bioengineering

Self Cite

275

174

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“…This is essential not only for controlled, reproducible, statistically relevant basic studies but also for the future routine manufacturing of tissues for clinical application [114,115]. Besides these global requirements, specific key criteria for 3D…”

Section: Bioreactors For Tissue Engineeringmentioning

confidence: 99%

“…tissue constructs based on cells and scaffolds interactions have to be met, including the proliferation of cells, seeding of cells on macro-porous scaffolds, nutrient (particularly oxygen) supply within the resulting tissue, and mechanical stimulation of the developing tissues [115]. .…”

Section: Bioreactors For Tissue Engineeringmentioning

confidence: 99%

Membrane supported scaffold architectures for tissue engineering

Bettahalli

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Prof. Dr. J. Vienken (Fresenius Medical Care, Germany) Dr. C. Legallais (Université de Technologie, France) Author -Narasimha Murthy Srivatsa BettahalliTitle -"Membrane supported scaffold architectures for tissue engineering"PhD Thesis, University of Twente, Enschede, The NetherlandsThe research described in this thesis was financially supported by STW (Utrecht, NL) (Project number -TKG.6716)Printed by: Gildeprint Drukkerijen, Enschede, The Netherlands Cover designed by NM Srivatsa BettahalliThe front cover shows dual flow glass bioreactor with 3D Free form fabricated scaffold integrated with hollow fibers (set-up artwork by Jonathan B Bennink of Tingle Visuals) and the back cover shows fluorescence microscope image of pre-labeled C2C12 cells cultured dynamically on multilayer cell-electrospun construct in dual flow perfusion bioreactor for 7 days (fluorescent image taken by Hemant Unadkat) and several SEM pictures at the top (PLLA hollow fiber, non-woven electrospun sheet and corrugated fiber) taken by the author. -3]. Along with the potential economic benefits from advanced tissue engineering technologies, reduced costs due to the availability of less expensive treatments for major medical problems is obvious, but indirect savings and dramatic improvements in treatment outcomes and quality of life for patients may prove to be even more important. There are several artificial tissues that are already being used for medical treatment (with limitations) which include fabricated skin, cartilage, blood vessels, bone, ligament and tendon [4][5][6]. It is also hoped that at least some of the whole organs like, liver, lung, kidney, pancreas, breast, intestine, etc. will become available off-the-shelf for treatment in the near future [7, 8]. MEMBRANE SUPPORTED SCAFFOLD ARCHITECTURES FOR TISSUE ENGINEERINGBesides the therapeutic application where the tissue is either grown in a patient (in-vivo) or outside the patient (in-vitro) and then transplanted to the patient, tissue engineering can have diagnostic applications where the tissue is made in-vitro and used for testing drug metabolism and uptake, toxicity, and pathogenicity [9][10][11][12]. The foundation of tissue engineering / regenerative medicine for either therapeutic or diagnostic applications is the ability to exploit living cells in a variety of ways.A tissue engineer first has to consider the function of the tissue -must it be strong? Elastic?Should it release certain proteins, like insulin from the pancreas, or filter toxins, like the kidneys? Then a 3D support must be designed that, when combined with cells, will eventually duplicate those functions, and continue to function properly in the body for years to come.Depending on the type of tissue, the design process can involve a variety of disciplines including mechanical / chemical engineering, molecular biology, physiology, medicine, polymer chemistry, and nanotechnology. Additionally, the scaffold can be constructed with or designed to release growth factors that can beneficially manipulate cell b...

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Stirred Tank Reactors for Cell Culture Technology

Pangarkar

2014

Design of Multiphase Reactors

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From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process

Cited by 3 publications

References 2 publications

Bioreactor design for tissue engineering

Bioreactor design for tissue engineering

Membrane supported scaffold architectures for tissue engineering

Stirred Tank Reactors for Cell Culture Technology

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