From an in vivo to an in vitro relative potency (IVRP) assay to fully characterize a multicomponent O-antigen based vaccine against Shigella

Necchi, Francesca; Giannelli, Carlo; Acquaviva, Alessandra; Alfini, Renzo; Monaci, Valentina; Arato, Vanessa; Rossi, Omar; Micoli, Francesca

doi:10.1016/j.carbpol.2023.120920

Cited by 3 publications

(1 citation statement)

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“…However, reproducible detection of the major OM protein classes, membrane-associated polysaccharides, and other associated antigens across independent experiments and in different organisms suggests that OMVs induce Ab responses to these antigens (Dalseg et al 1999 ; Leduc et al 2020 ; Viviani et al 2023 ; Wedege et al 2007 ). Shigella OMVs have also been well studied, and pAb responses to major OM antigens such as LPS, porins, and secretion system components have been observed (Mancini et al 2021 ; Necchi et al 2023 ). OMVs from several other gram-negative bacteria have been shown to induce pAb responses against membrane antigens (Table 1 , Supplementary Table S1 ).…”

Section: Uses Of Omvs As Immunogensmentioning

confidence: 99%

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens

Lei,

Azmat,

Henry

et al. 2024

Appl Microbiol Biotechnol

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Bacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived from the periplasmic space, the bacterial outer membrane, and possibly other compartments. OMVs can be purified from bacterial culture supernatants, and by genetically manipulating the bacterial cells that produce them, they can be engineered to harbor cargoes and/or display molecules of interest on their surfaces including antigens that are immunogenic in mammals. Since OMV bilayer-embedded components presumably maintain their native structures, OMVs may represent highly useful tools for generating antibodies to bacterial outer membrane targets. OMVs have historically been utilized as vaccines or vaccine constituents. Antibodies that target bacterial surfaces are increasingly being explored as antimicrobial agents either in unmodified form or as targeting moieties for bactericidal compounds. Here, we review the properties of OMVs, their use as immunogens, and their ability to elicit antibody responses against bacterial antigens. We highlight antigens from bacterial pathogens that have been successfully targeted using antibodies derived from OMV-based immunization and describe opportunities and limitations for OMVs as a platform for antimicrobial antibody development. Key points • Outer membrane vesicles (OMVs) of gram-negative bacteria bear cell-surface molecules • OMV immunization allows rapid antibody (Ab) isolation to bacterial membrane targets • Review and analysis of OMV-based immunogens for antimicrobial Ab development

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