From a plant secretion to the promising bone grafts: Cryogels of silicon-integrated quince seed mucilage by microwave-assisted sol–gel reaction

Yilmaz, Hilal Deniz; Cengiz, Uğur; Arslan, Yavuz Emre; Kıran, Fadime; Ceylan, Ahmet

doi:10.1016/j.jbiosc.2020.11.008

Cited by 13 publications

(53 citation statements)

References 78 publications

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“…A higher storage modulus ( G’ ) over loss modulus ( G″ ) indicated a gel-like behavior for the B-Si-Col-HK hydrogel. This phenomenon is related to the higher elastic component rather than the viscous component . In our study, the stable self-standing properties and well-developed 3D networks of the B-Si-Col-HK structure showed more gel-like rather than liquid-like behavior because of having about 100 times higher G″ values according to the Col-HK hydrogels .…”

Section: Resultsmentioning

confidence: 49%

“…In addition, the temperature sweep test of the B-Si-Col-HK hydrogel was carried out at 4–40 °C and 1 °C/min sweep rate (Figure S2B). In this respect, we can assume that the B-Si-Col-HK hydrogel could successfully be used in a wide spectrum, including in body temperature conditions, without any change in its 3D gel-like structure …”

Section: Resultsmentioning

confidence: 99%

“…Initially, silica-based sol was prepared for the synthesis of inorganic–organic hybrid hydrogels. Reactive TEOS (rTEOS), which is a source of silica, was prepared as in our previously published study . Briefly, TEOS (3 mL), HCl (0.1 M, 0.18 mL), and Milli-Q water (0.6 mL) (Milli Direct-Q 3UV, 18 MΩ cm –1 at 25 °C, Merck-Millipore, Germany) were mixed vigorously for 5 min in a glass vial at RT.…”

Section: Methodsmentioning

confidence: 99%

“…I/HC studies were conducted to identify the osteogenic differentiation of the seeded cells, which was triggered due to the structural and/or biochemical internal signals of Col-HK bioscaffolds and B-Si-Col-HK cryogels . The I/HC analyses were performed as previously described . The specimens were stained with hematoxylin and eosin (H&E) and Masson’s trichrome to observe the cellular response on the scaffolds.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, Kakkar and Madhan fabricated a keratin/silica hydrogel exploiting TEOS as a precursor and evaluated its effectiveness for soft tissue engineering applications . On the other hand, we developed 3D cryogels from silicon-integrated quince seed mucilage (QSM) via the sol–gel method and evaluated the osteogenic differentiation capacity of human adipose mesenchymal stem cells (hAMSCs) on such constructs by in vitro tests …”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Silica-Based Boron-Incorporated Collagen/Human Hair Keratin Hybrid Cryogels with the Potential Bone Formation Capability

Cal

Arslan

Derkuş

et al. 2021

ACS Appl. Bio Mater.

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Tissue engineering and regenerative medicine have evolved into a different concept, the so-called clinical tissue engineering. Within this context, the synthesis of next-generation inorganic–organic hybrid constructs without the use of chemical crosslinkers emerges with a great potential for treating bone defects. Here, we propose a sophisticated approach for synthesizing cost-effective boron (B)- and silicon (Si)-incorporated collagen/hair keratin (B-Si-Col-HK) cryogels with the help of sol–gel reactions. In this approach, collagen and hair keratin were engaged with a B-Si network using tetraethyl orthosilicate as a silica precursor, and the obtained cryogels were characterized in depth with attenuated total reflectance-Fourier transform infrared spectroscopy, solid-state NMR, X-ray diffraction, thermogravimetric analysis, porosity and swelling tests, Brunauer–Emmett–Teller and Barrett–Joyner–Halenda analyses, frequency sweep and temperature-dependent rheology, contact angle analysis, micromechanical tests, and scanning electron microscopy with energy dispersive X-ray analysis. In addition, the cell survival and osteogenic features of the cryogels were evaluated by the MTS test, live/dead assay, immuno/histochemistry, and quantitative real-time polymerase chain reaction analyses. We conclude that the B-Si-networked Col-HK cryogels having good mechanical durability and osteoinductive features would have the potential bone formation capability.

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Section: Resultsmentioning

confidence: 49%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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