Frog Virus 3 Replication: Electron Microscope Observations on the Sequence of Infection in Chick Embryo Fibroblasts

Kelly, D. C.

doi:10.1099/0022-1317-26-1-71

Cited by 37 publications

(26 citation statements)

References 2 publications

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“…The extensive microtubule formation and cytoplasmic inclusions seen in FV3-infected cells (Kelly & Atkinson 1975) were not found in TGIV-infected cells. The distribution of chromatin in a TGIV-infected cell is different from that in an FV3-infected cell, and is not arranged along the inner nuclear membrane as with FV3 (Kelly 1975). Although the pathology of WSIV infection includes cell hypertrophy, it does not reach the same extent as in TGIV infection (Watson et al 1998).…”

Section: Discussionmentioning

confidence: 91%

Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)

Chao¹,

Chen²,

Lai³

et al. 2004

Dis. Aquat. Org.

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Section: Discussionmentioning

confidence: 91%

Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)

Chao¹,

Chen²,

Lai³

et al. 2004

Dis. Aquat. Org.

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“…In an earlier paper (Kelly, 1975) virus particles were observed (as thin sections) budding in two situations; either as particles whose envelopment was apparently being completed at the plane of the plasma membrane, or, less frequently, as particles fitting snugly at the end of long protrusions. This is confirmed by surface replica and scanning electron microscopy, but it is not resolved whether this represents two distinct phenomena.…”

Section: Discussionmentioning

confidence: 99%

“…Aberrant forms of frog virus 3 are often encountered in infection (Darlington et al 1966; Bragaglia, Campadelli-Fiume & La Placa, 1974;Kelly, 1975). A focus containing aberrant forms is shown in Fig.…”

Section: Frozen Fractured Infected B H K Cellsmentioning

confidence: 99%

“…Ioa shows a cross fracture of the nuclear membranes 48 h after infection again demonstrating that nuclear pores are smaller than virus particles. In thin sections early cytoplasmic foci of frog virus 3 infection typically comprise an electron translucent area with virus particles and mitochondria scattered at the periphery (Kelly, 1975). Such a focus, frozen fractured, is shown in Fig.…”

Section: Frozen Fractured Infected B H K Cellsmentioning

confidence: 99%

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Frog Virus 3 Replication: Electron Microscope Observations on the Terminal Stages of Infection in Chronically Infected Cell Cultures

Kelly

Atkinson

1975

Journal of General Virology

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SUMMARYAn examination of BHK, CEF, and FHM cells chronically infected with frog virus 3 has been made by scanning and transmission (thin section, freeze fracture, and surface replica) electron microscopy. With minor differences the pattern of virus development is similar in all three cell lines. Virus particles were detected in cell nuclei which subsequently became degenerate very late in infection. Three inclusions were associated with frog virus 3 cytoplasmic loci of infection; /amelia structures, extensive microtubule formation (in BHK and FHM ceils), and linear crystalline structures. The last two structures may play a role in creating or maintaining the cell rounding c.p.e, revealed by scanning electron microscopy. Very late in infection most BHK and FHM, but not CEF, cells are stripped of the plasma membrane. Replicas of frozen fractured BHK cells featured cytoplasmic loci of infection, budding at the plasma membrane, and showed that at early times when virus is detected in the nucleus, the nuclear membranes are intact and morphologically unaltered. Budding at the plasma membrane was better resolved by scanning and as surface replicas. This demonstrated that sparse to profuse localized budding occurred. Frequently virus particles were located singly, or as multiples, at the end of, or along, cytoplasmic protrusions which occur both on the body of the cells and at the cytoplasmic/coverslip 'interface'.

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“…On the other hand, the ultrastructural features of FV 3 maturation have been reported by sevelal authors (Dartington, Granoff & Breeze, I966;Bingen-Brendel, Tripier & Kirn, 1971;Tripier et aL I974;Kelly, 1975). Until now, however, there has been no study in which the biochemical and morphological steps of virus biosynthesis have been correlated.…”

Section: Introductionmentioning

confidence: 90%

Frog Virus 3 Morphogenesis: Effect of Temperature and Metabolic Inhibitors

Tripier

Braunwald

Markovic³

et al. 1977

Journal of General Virology

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SUMMARYThe different stages of frog virus 3 (FV 3) morphogenesis have been investigated in chick embryo fibroblasts infected at an optimal temperature for virus growth (29 °C). The metabolic requirements for morphogenesis were determined by adding inhibitors of macromolecular synthesis at different periods in the virus growth cycle. The effect of a non-permissive temperature for FV 3 replication (37 °C) was also studied in shift up experiments. The following results were obtained: (I) when DNA replication was inhibited, neither immature nor mature virus particles appeared; (2) continuous protein synthesis was required for every stage of virus morphogenesis. However, the assembly of virions into paracrystalline arrays seemed to be a passive phenomenon. (3) Continuous mRNA transcription was not necessary for assembly of capsid constituents, although most of these capsids appeared empty; there was also a striking increase in the number of aberrant virus structures. (4) If infected cells were shifted to a non-permissive temperature, virus maturation was completely inhibited.

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Frog Virus 3 Replication: Electron Microscope Observations on the Sequence of Infection in Chick Embryo Fibroblasts

Cited by 37 publications

References 2 publications

Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)

Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)

Frog Virus 3 Replication: Electron Microscope Observations on the Terminal Stages of Infection in Chronically Infected Cell Cultures

Frog Virus 3 Morphogenesis: Effect of Temperature and Metabolic Inhibitors

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