Frictional properties of regenerated cartilage in vitro

Morita, Yusuke; Tomita, Naohide; Aoki, Hideyuki; Sonobe, Masato; Wakitani, Shigeyuki; Tamada, Yasushi; Suguro, Toru; Ikeuchi, Ken

doi:10.1016/j.jbiomech.2004.10.031

Cited by 61 publications

(50 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By day 38, the difference was insignificant as the interface now consisted entirely of new ECM, which was compositionally the same on both scaffolds. Furthermore, these constructs displayed equilibrium coefficients of friction that were similar to those of native cartilage, as well as other engineered cartilage constructs, by day 38 (40)(41)(42)(43)(44)(45).…”

Section: Discussionmentioning

confidence: 74%

Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing

Moutos

Glass

Compton

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

Biological resurfacing of entire articular surfaces represents an important but challenging strategy for treatment of cartilage degeneration that occurs in osteoarthritis. Not only does this approach require anatomically sized and functional engineered cartilage, but the inflammatory environment within an arthritic joint may also inhibit chondrogenesis and induce degradation of native and engineered cartilage. The goal of this study was to use adult stem cells to engineer anatomically shaped, functional cartilage constructs capable of tunable and inducible expression of antiinflammatory molecules, specifically IL-1 receptor antagonist (IL-1Ra). Large (22-mm-diameter) hemispherical scaffolds were fabricated from 3D woven poly(ε-caprolactone) (PCL) fibers into two different configurations and seeded with human adipose-derived stem cells (ASCs). Doxycycline (dox)-inducible lentiviral vectors containing eGFP or IL-1Ra transgenes were immobilized to the PCL to transduce ASCs upon seeding, and constructs were cultured in chondrogenic conditions for 28 d. Constructs showed biomimetic cartilage properties and uniform tissue growth while maintaining their anatomic shape throughout culture. IL-1Ra–expressing constructs produced nearly 1 µg/mL of IL-1Ra upon controlled induction with dox. Treatment with IL-1 significantly increased matrix metalloprotease activity in the conditioned media of eGFP-expressing constructs but not in IL-1Ra–expressing constructs. Our findings show that advanced textile manufacturing combined with scaffold-mediated gene delivery can be used to tissue engineer large anatomically shaped cartilage constructs that possess controlled delivery of anticytokine therapy. Importantly, these cartilage constructs have the potential to provide mechanical functionality immediately upon implantation, as they will need to replace a majority, if not the entire joint surface to restore function.

show abstract

Section: Discussionmentioning

confidence: 74%

Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing

Moutos

Glass

Compton

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

show abstract

“…This agrees with the hypothesis that the sliding tests showed no sign of interstitial fluid pressurization. Other studies 10,16,19 have reported interstitial …”

Section: Figmentioning

confidence: 83%

“…Under these conditions, the frictional response was solely or mostly given by the response of the ECM rather than the scaffold. 10,16,19 Previous studies have shown a higher equilibrium friction coefficient for tissue-engineered articular cartilage, 10,16 while others have shown a lower l eq compared to native articular cartilage. 17,19 These variances can be based on testing conditions/configurations, the type of scaffold used, and cell culture duration/conditions, including the type of cell.…”

mentioning

confidence: 99%

Effects of Fiber Orientation on the Frictional Properties and Damage of Regenerative Articular Cartilage Surfaces

Accardi

McCullen

Callanan

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

Articular cartilage provides a low-friction, wear-resistant surface for diarthrodial joints. Due to overloading and overuse, articular cartilage is known to undergo significant wear and degeneration potentially resulting in osteoarthritis (OA). Regenerative medicine strategies offer a promising solution for the treatment of articular cartilage defects and potentially localized early OA. Such strategies rely on the development of materials to restore some aspects of cartilage. In this study, microfibrous poly(e-caprolactone) scaffolds of varying fiber orientations (random and aligned) were cultured with bovine chondrocytes for 4 weeks in vitro, and the mechanical and frictional properties were evaluated. Mechanical properties were quantified using unconfined compression and tensile testing techniques. Frictional properties were investigated at physiological compressive strains occurring in native articular cartilage. Scaffolds were sheared along the fiber direction, perpendicular to the fiber direction and in random orientation. The evolution of damage as a result of shear was evaluated via white light interferometry and scanning electron microscopy. As expected, the fiber orientation strongly affected the tensile properties as well as the compressive modulus of the scaffolds. Fiber orientation did not significantly affect the equilibrium frictional coefficient, but it was, however, a key factor in dictating the evolution of surface damage on the surface. Scaffolds shear tested perpendicular to the fiber orientation displayed the highest surface damage. Our results suggest that the fiber orientation of the scaffold implanted in the joint could strongly affect its resistance to damage due to shear. Scaffold fiber orientation should thus be carefully considered when using microfibrous scaffolds.

show abstract

“…This is critical because damage due to mechanical loading is not always apparent at the tissue level (Needham et al 1991). Morita et al (2006) previously reported a method for friction testing on living, engineered tissue (i.e. cartilage constructs).…”

Section: Introductionmentioning

confidence: 99%

A novel method for low load friction testing on living cells

et al. 2007

View full text Add to dashboard Cite

A low load tribology technique for studying the effects of friction on living cells was developed. Results show a direct relationship between the coefficient of friction (COF) and the extent of cell damage. The COF, mu, for a glass pin on an intact layer of human corneal epithelial cells is determined to be on the order of mu = 0.05 +/- 0.02 (n = 16). The correlations between applied normal load and extent of cell damage, as well as between number of reciprocation cycles and cell damage, are reported. It is also found that cell damage can occur when a loading force as low as 0.5 mN is applied, although the cells appear to be intact.

show abstract

Frictional properties of regenerated cartilage in vitro

Cited by 61 publications

References 16 publications

Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing

Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing

Effects of Fiber Orientation on the Frictional Properties and Damage of Regenerative Articular Cartilage Surfaces

A novel method for low load friction testing on living cells

Contact Info

Product

Resources

About