Fribrinolytic activity and gas production by Pleurotus ostreatus-IE8 and Fomes fomentarius - EUM1 in bagasse cane

Sánchez-Santillán, Paulino; Meneses-Mayo, Marcos; Miranda‐Romero, Luis Alberto; Santellano‐Estrada, Eduardo; Alarcón-Zúñiga, Baldomero

doi:10.21897/rmvz.6

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…At 72 h of incubation, S1 with 21 d had a lower gas production (p0.05), representing 73.88% of the total gas production averaged by the rest of the evaluated silages (63.56 mL of gas g 1 DM; Table 1). The levels of gas production were obtained from the anaerobic fermentation of soluble or structural carbohydrates, but silages originate from anaerobic fermentation (Sánchez-Santillán et al, 2015). Antolín et al (2009) reported higher values than those observed in this study, with a production of 217.5 mL of gas g 1 DM at 96 h of incubation in hybrid corn silages.…”

Section: Resultscontrasting

confidence: 52%

In vitro gas and methane production and dry matter degradation of pumpkin (Cucurbita argyrosperma) silages with pangola grass (Digitaria decumbens) hay

Sánchez-Santillán¹,

Torres-Salado²,

Herrera-Pérez³

et al. 2020

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Objetivo: Determinar la producción de gas, metano y degradación de la materia seca in vitro de ensilados elaborados con cascara y pulpa de calabaza (CPC; Cucurbita argyrosperma), heno de pasto pangola (Digitaria decumbens) y como aditivos urea y dos porcentajes de inclusión de melaza fermentados durante 14 y 21 días. Metodología: Los ensilados (2 Kg) fueron: E1 = 72.5% de CPC, 22.5% de pasto pangola, 3% de melaza y 2% de urea; E2 = 72.5% de CPC, 19.5% de pasto pangola, 6% de melaza y 2% de urea. Ambos ensilados se fermentaron por 14 y 21 días. Se evaluó la producción de gas, metano (CH4), conteo de bacterias totales y degradación de materia seca (DEGMS). El diseño experimental fue un diseño completamente al azar con arreglo factorial 2 x 2 con tipos de ensilado y tiempo de fermentación como factores. Resultados: El E1 a los 21 d mostró la menor producción de gas a las 72 h (46.96 mL g-1 MS) y menor DEGMS (35.78%; p ? 0.05). La producción de CH4 y conteo de bacterias totales no mostraron diferencias (p > 0.05) entre tipos de ensilado, ni tiempos de fermentación. Limitaciones del estudio: La inclusión de 3% de melaza con un tiempo de fermentación de 21 días presentó la menor producción de gas y degradación de la materia seca en ensilados con cascara y pulpa de calabaza y heno de pasto pangola. Conclusiones: Los ensilados elaborados con cascara y pulpa de calabaza son una alternativa viable para conservar y producir alimento para rumiantes en la época de sequía; así mismo usar adecuadamente materiales potencialmente contaminantes como la cascara y pulpa de calabaza.

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Section: Resultscontrasting

confidence: 52%

In vitro gas and methane production and dry matter degradation of pumpkin (Cucurbita argyrosperma) silages with pangola grass (Digitaria decumbens) hay

Sánchez-Santillán¹,

Torres-Salado²,

Herrera-Pérez³

et al. 2020

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“…Además, la producción de biogás hasta las 24 h se relaciona con la fermentación de carbohidratos no estructurales o solubles, después de este tiempo se fermentan los carbohidratos estructurales, y a las 48 h se muestra el desdoblamiento de los carbohidratos estructurales entrelazados con lignina (Sánchez-Santillán et al, 2015). De esta manera, el origen de los CBC modifica el tipo de carbohidratos que se fermentan (Figura 1).…”

Section: Resultados Y Discusiónunclassified

PRODUCCIÓN DE BIOGÁS Y CARACTERÍSTICAS FERMENTATIVAS in vitro DE CONSORCIOS BACTERIANOS CELULOLÍTICOS RUMINALES OBTENIDOS DE DIFERENTES FIBRAS

Hernández-Sánchez

Herrera-Pérez

Garrido³

et al. 2022

Trop Subtrop Agroecosyst

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Background. Cellulolytic bacterial consortia (CBC) serve as additives to improve fiber degradation in ruminants, since they improve biogas production and in vitro fermentation characteristics. Objective. To evaluate the biogas production and fermentative characteristics in vitro of mulato grass inoculated with ruminal CBC obtained from ground sawdust, ground mulato grass or whole stem as substrates in the selective culture medium. Methodology. The CBC were obtained from ruminal fluid from a Suiz-Bu cow fitted with a ruminal cannula, which was transferred six times in a selective anaerobic medium. The test consisted of preparing sterile biodigesters with 0.5 g of mulatto grass with 63 d of regrowth, 45 mL of culture medium. The inoculation was with 5 mL of a type of CBC. The biodigesters were incubated 72 h at 39 °C. Biogas production was measured at 3, 6, 9, 12, 24, 48 and 72 h. In the culture media, ammonia nitrogen (N-NH3), total bacterial count, cellulase activity, pH, dry matter degradation (DMD) and neutral detergent fiber degradation (NDFD) were determined at 12, 24, 48 y 72 h. In the biogas production a completely random design was used; while in the rest of the variables a completely randomized design was carried out with a 3x4 factorial arrangement, with fiber source and incubation time as factors. Results. The biogas production of the CBC obtained from the ground grass showed higher (p<0.05) accumulated production in all the evaluated times. The CBC obtained from sawdust increased the biogas production by 175% from 24 to 48 h and by 313% from 48 to 72 h. There was an interaction effect (p <0.05) on DMD, NDFD, N-NH3, total bacterial count and cellulase enzymatic activity. The CBC obtained from ground mulatto grass at 72 h increased (p <0.05) the DMD and NDFD. The CBC obtained from sawdust increased DMD by 19.1% and NDFD by 33% from 48 to 72 h. The concentration of bacteria in the observed interactions is within the range of the ruminal ecosystem. The highest (p<0.05) content of N-NH3 was determined when the CBC obtained from grass stem with 12, 24 and 72 h of incubation were used. Implications. Using the same fiber source to obtain the CBCs improves the degradation of said fiber. Conclusion. The size and source of fiber are decisive in the type of bacteria that make up the cellulolytic bacterial consortia of ruminal origin under the conditions of this in vitro test.

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“…Las bolsas con el residuo se secaron 24 h a 60 °C en un horno de secado. La DMS se calculó con la formula DMS (%) = (muestra inicialmuestra residual / muestra inicial) * 100 (Sánchez-Santillán et al, 2015;Hernández-Morales et al, 2018). Las bolsas ANKOM ® se sellaron a calor y se determinó el contenido de FDN (Van Soest et al, 1991).…”

Section: Ensayo In Vitrounclassified

“…La medición parcial y acumulada del biogás permite inferir la disponibilidad de carbohidratos para los microorganismos ruminales durante el proceso de fermentación ruminal in vitro usando la técnica de producción de gas (Texta et al, 2019). De modo que la producción de biogás parcial en las primeras 24 h indica la disponibilidad de carbohidratos solubles o no estructurales del ensilado de papaya de desecho, mientras que a partir de las 24 h comienza la fermentación de carbohidratos estructurales no adheridos a lignina y después de las 48 h se fermentan aquellos carbohidratos estructurales adheridos a lignina (Sánchez-Santillán et al, 2015;Texta et al, 2019;Torres-Salado et al, 2019). Esto permite inferir, la adición de melaza a los ensilados de papaya de desecho del presente estudio afecta la disponibilidad de los carbohidratos durante la fermentación ruminal, ya que aumenta la producción de biogás y metano en términos generales.…”

Section: Días Fermentaciónunclassified

CARACTERÍSTICAS DE CALIDAD, QUÍMICAS Y FERMENTATIVAS in vitro DE ENSILADOS DE PAPAYA (Carica papaya L) DE DESECHO Y HENO DE PASTO ESTRELLA (Cynodon nlemfluensis)

Sánchez-Santillán

Marcial²,

Saavedra-Jiménez

et al. 2021

Trop Subtrop Agroecosyst

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Background. Ripe papaya that does not meet standards for human consumption has the potential to be ensiled with star grass as a moisture absorber, which has the potential as an unconventional ruminant feed. Objective. To determine the quality, nutritional, and in vitro fermentative characteristics of silages made with milled ripe papaya (Carica papaya L) and star (Cynodon nlemfluensis) grass hay as moisture absorber, adding three levels of molasses during 21 and 28 days of lactic fermentation. Methodology. The silos (1.5 Kg) were made with 75% ripe papaya, 25% star grass hay, added with 2% urea and 0, 3 and 6% cane molasses; the silos were fermented for 21 and 28 d. The quality characteristics (pH, lactic acid (LA), ammonia nitrogen (N-NH3) and dry matter (DM)), chemical characteristics (crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and ashes (As)) and in vitro fermentation (partial and accumulated production of biogas and methane (CH4), degradation of dry matter (DDM) and degradation of neutral detergent fiber (DNDF) were evaluated). The statistical analysis was a 3x2 factorial arrangement within a completely randomized design, using level of molasses addition and fermentation time as factors. Results. pH, LA, CP and partial biogas at 24 and 48 h showed interaction between factors (p<0.05). Silages with 0% molasses showed lower content of DM, As, accumulated biogas, partial CH4 at 24 h, DDM; as well as, higher NDF, partial CH4 at 72 h. The silages with 6% molasses presented higher DM, As, partial CH4 at 24 h, accumulated CH4 and DDM; in addition, lower NDF, ADF, partial CH4 at 72 h. Silages with 21 d had lower N-NH3, NDF, Ce, Biogas and accumulated CH4, partial CH4 at 24 h and 72 h than at 28 d (p<0.05). The highest pH value was in silages with 28 d and 6% molasses and the highest LA in 28 d silages with 0% molasses (p<0.05). Implications. Ripe papaya silage does not require cane molasses as an additive; there is no effect on the opening of the silos at 21 or 28 d of lactic fermentation. Conclusion. Silage made with ripe papaya and star grass hay as moisture absorber, with urea does not require sugar cane molasses as an additive to maintain its quality, chemical and in vitro fermentation characteristics, which allows inferring that it is an unconventional alternative in the feeding of ruminants.

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Fribrinolytic activity and gas production by Pleurotus ostreatus-IE8 and Fomes fomentarius - EUM1 in bagasse cane

Cited by 10 publications

References 11 publications

In vitro gas and methane production and dry matter degradation of pumpkin (Cucurbita argyrosperma) silages with pangola grass (Digitaria decumbens) hay

In vitro gas and methane production and dry matter degradation of pumpkin (Cucurbita argyrosperma) silages with pangola grass (Digitaria decumbens) hay

PRODUCCIÓN DE BIOGÁS Y CARACTERÍSTICAS FERMENTATIVAS in vitro DE CONSORCIOS BACTERIANOS CELULOLÍTICOS RUMINALES OBTENIDOS DE DIFERENTES FIBRAS

CARACTERÍSTICAS DE CALIDAD, QUÍMICAS Y FERMENTATIVAS in vitro DE ENSILADOS DE PAPAYA (Carica papaya L) DE DESECHO Y HENO DE PASTO ESTRELLA (Cynodon nlemfluensis)

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