Frex and FrexH

Monitoring intracellular redox metabolism with genetically encoded fluorescent sensors

“…cpYFP与Frex具有相似的 pH敏感性, 在运用Frex时可以通过平行测定对照探针 cpYFP, 消除pH的干扰 [13,38,39] . 此外, 也可利用pH敏感 的化学染料 [44] 或红色的pH荧光蛋白探针 [45] 进行pH校 [39,46,47] 精 [39] . 基于大量实验数据, Zhao等 人 [39] 提出了胞浆中的NADH对细胞生理条件改变非常 敏感, 而线粒体有很强的维持生理NADH稳态的观点.…”

Real-Time Assessment of the Metabolic Profile of Living Cells with Genetically Encoded NADH Sensors

“…We estimated that the Frex-Mit-bound NADH is less than 12% of the total mitochondrial NADH in resting cells. According to these calculations, Frex sensors are unlikely to have a major impact on the total NADH concentration and the metabolic status of the cells in which they are expressed (Zhao and Yang, 2012). …”

Discriminating changes in intracellular NADH/NAD+ levels due to anoxicity and H2 supply in R. eutropha cells using the Frex fluorescence sensor

“…SoNar ) have been optimized for mammalian cell lines. Importantly, the total cellular NADH/NAD + pools and their ratio may differ substantially in mammalian and bacterial cells (Bennett et al, 2009;Zhao and Yang, 2012;Zhou et al, 2011). Indeed, the Peredox sensor (designed to report the [NADH]/[NAD + ] ratio) was found to operate close to its saturation level when produced in R. eutropha cells grown under aerobic conditions, because its NADH sensitivity is in the lower nanomolar range (Tejwani et al, 2017;.…”