FRET-based fluorescent nanoprobe platform for sorting of active microorganisms by functional properties

Luo, Yeshen; Liu, Fei; Li, Enze; Fang, Yun; Zhao, Gang; Dai, Xin; Li, Jianjun; Wang, Bin; Xu, Meiying; Liao, Bing; Sun, Guoping

doi:10.1016/j.bios.2019.111832

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…Previous studies have shown that the fluorescence of the probe recovers when the azo moiety is degraded by microorganisms ( Luo et al, 2020 ). Therefore, GQDs-M can serve as an off-on switch probe for monitoring azo-respiration functional bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…E. coli S17-1 could not perform azo-respiration, and S. decolorationis S12, Lysinibacillus sp. GY32, B. terrae RA9, B. thiooxidans BI-42, L. pakistanensis NCCP-54, and N. coeliaca DSM 44595 with azo-degradation function ( Luo et al, 2020 ) were employed. As shown in Supplementary Figure 8 , all strains except E. coli can completely degrade methyl red at a concentration of 0.1 M within 8 h. All strains were grown in a culture medium containing GQDs-M (10 μg/mL), and confocal microscopic images were obtained using a 488-nm laser at a low voltage.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study reported by our research group, a non-toxic silica nanoparticle probe in combination with fluorescence-activated cell sorting was used to detect and screen bacteria with degrading functions ( Luo et al, 2020 ). To improve the screening efficiency for identifying these bacteria from the environment, graphene quantum dot (GQD) probes have been used as suitable alternatives to silica nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

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Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

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Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environmental samples. It is suggested that the estimation of environmental functional microbial populations based on bioimaging will be a new method for rapid preliminary assessment of environmental pollution levels. In combination with a visual single-cell sorter, the target bacteria in the environmental samples could be intuitively screened at the single-cell level in 17 bacterial strains, including the positive control Shewanella decolorationis S12, and were isolated from environmental samples. All of these showed an azo degradation function, indicating the high accuracy of the single-cell sorting strategy using the GQDs-M. Furthermore, among the bacteria isolated, two strains of Bacillus pacificus and Bacillus wiedmannii showed double and triple degradation efficiency for methyl red compared to the positive control (strain S12). This strategy will have good application prospects for finding new species or high-activity species of specific functional bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

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Section: Regulation Methods For Azobenzenementioning

confidence: 99%

“…In addition, azobenzene has significant applications in bioimaging. In many optical probes, azobenzene can be used as a hypoxia‐sensitive part [ 129 ] (Figure 5b). These hypoxia‐sensitive probes usually are constructed on the basis of fluorescence resonance energy transfer (FRET), in which the FRET donor and FRET acceptor are linked by azobenzene.…”

Section: Regulation Methods For Azobenzenementioning

confidence: 99%

Advances in Application of Azobenzene as a Trigger in Biomedicine: Molecular Design and Spontaneous Assembly

et al. 2021

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Azobenzene is a well‐known derivative of stimulus‐responsive molecular switches and has shown superior performance as a functional material in biomedical applications. The results of multiple studies have led to the development of light/hypoxia‐responsive azobenzene for biomedical use. In recent years, long‐wavelength‐responsive azobenzene has been developed. Matching the longer wavelength absorption and hypoxia‐response characteristics of the azobenzene switch unit to the bio‐optical window results in a large and effective stimulus response. In addition, azobenzene has been used as a hypoxia‐sensitive connector via biological cleavage under appropriate stimulus conditions. This has resulted in on/off state switching of properties such as pharmacology and fluorescence activity. Herein, recent advances in the design and fabrication of azobenzene as a trigger in biomedicine are summarized.

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Highly Sensitive and Selective Plasmonic Sensing Platforms

Saylan

Deni̇zli̇

2021

Plasmonic Sensors and Their Applications

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FRET-based fluorescent nanoprobe platform for sorting of active microorganisms by functional properties

Cited by 14 publications

References 45 publications

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Advances in Application of Azobenzene as a Trigger in Biomedicine: Molecular Design and Spontaneous Assembly

Highly Sensitive and Selective Plasmonic Sensing Platforms

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