FRET-based acrylic nanoparticles with dual-color photoswitchable properties in DU145 human prostate cancer cell line labeling

Rad, Jaber Keyvan; Mahdavian, Ali Reza; Khoei, Samideh; Esfahani, Azam Janati

doi:10.1016/j.polymer.2016.06.042

Cited by 36 publications

(10 citation statements)

References 28 publications

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Section: Results and Discussionmentioning

confidence: 99%

“…Cytotoxicity of DU145 prostate cancer cells to PFAM nanocomposite particles was assessed by the trypan blue dye exclusion assay. 54 In summary, DU145 prostate cancer cells were seeded into a 24-well plate (Nunc, Roskilde, Denmark) at a density of 1 × 10 4 cells/well. After 24 h, the culture medium was replaced with the same volume of culture medium, containing different concentrations of PFAM nanocomposite particles (0−10 mg/mL), and cytotoxicity was assessed after 24 h of incubation at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In order to assess the effect of nanocarriers without 5-FU and PFAM nanocomposite particles on DU145 cancer cell viability, particles were purified through dialysis first, and then the trypan blue dye exclusion assay was conducted. 54 DU145 cell viabilities after treatment for 24 h with different concentrations of the above both particles were evaluated next. 50 and 10% inhibitory concentrations (IC50, IC10) were calculated and found to be 0.73 and 0.46 mg/mL for the nanocarriers without 5-FU and 0.62 and 0.39 mg/mL for PFAM nanocomposite particles, respectively (Figure 8a).…”

Section: Dual Magneto and Photothermal Responsesmentioning

confidence: 99%

“…Cytotoxicity of DU145 prostate cancer cells to PFAM nanocomposite particles was assessed by the trypan blue dye exclusion assay . In summary, DU145 prostate cancer cells were seeded into a 24-well plate (Nunc, Roskilde, Denmark) at a density of 1 × 10 4 cells/well.…”

Section: Experimental Sectionmentioning

confidence: 99%

See 3 more Smart Citations

Controlled Release and Photothermal Behavior of Multipurpose Nanocomposite Particles Containing Encapsulated Gold-Decorated Magnetite and 5-FU in Poly(lactide-co-glycolide)

Rad

Alinejad

Khoei

et al. 2019

ACS Biomater. Sci. Eng.

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Nowadays, many research studies have been conducted to prepare multidisciplinary probes in drug delivery systems and cancer therapy with high performance and minimum side effects. Here, poly(lactide-co-glycolide) (PLGA) nanocomposite particles containing 5-fluorouracil (5-FU) and gold-decorated magnetite nanoparticles with a raspberry-like morphology were designed and prepared as a novel and anticancer probe. For this reason, Fe 3 O 4 nanoparticles were synthesized by a coprecipitation method and modified with (3-mercaptopropyl) trimethoxysilane for the deposition of gold nanoparticles. Then, they were embedded in the PLGA matrix alone and accompanied by 5-FU with 92 and 88% loading efficiencies, respectively, through a multiple emulsion solvent evaporation method. Chemical structure and composition of the prepared samples in each step were completely characterized by several techniques. The morphology of the nanocomposite particles was assessed by field emission scanning electron microscopy, high-resolution transmission electron microscopy, and selected area electron diffraction patterns, and their particle size and colloidal stability after 1 week were evaluated by dynamic light scattering. Because of the coexistence of gold and Fe 3 O 4 nanoparticles, the final probe provided enhanced dual magneto and photothermal responses by increasing the temperature up to 42.7 °C under 5 min external alternating magnetic field and to 42.1 °C within just 1 min near-infrared irradiation at 808 nm. Trypan blue dye exclusion assays showed that they are biocompatible with reasonable toxicity (IC50 of 0.62 mg/mL) with respect to DU145 prostate cancer cells. Drug release profile of the 5-FU-loaded nanocomposite particles demonstrated their controlled release at 37 °C in phosphate-buffered saline solution. These indicate multidisciplinary characteristics of such particles in cancer therapy by photothermal, magnetic hyperthermia, and chemotherapy according to the presence of various active components.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dual Magneto and Photothermal Responsesmentioning

confidence: 99%

Section: Experimental Sectionmentioning

confidence: 99%

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Controlled Release and Photothermal Behavior of Multipurpose Nanocomposite Particles Containing Encapsulated Gold-Decorated Magnetite and 5-FU in Poly(lactide-co-glycolide)

Rad

Alinejad

Khoei

et al. 2019

ACS Biomater. Sci. Eng.

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“…As a result of the differences between the SP and MC forms, and the ability to switch between them repeatedly by application of an external stimulus, SP molecules have received widespread attention for their use in many various dynamic systems, including SP-functionalised polymers, biopolymers, inorganic nanoparticles, carbon nanomaterials and solid surfaces—many of which have potential biological applications ( Klajn, 2014 ; Cardano et al, 2019 ); notably, in cell tracking and labelling ( Keyvan Rad et al, 2015 ; Keyvan Rad et al, 2016 ; Cong et al, 2021 ), photothermal therapy ( Keyvan Rad et al, 2018 ) and cell sheet engineering ( Karimipour et al, 2021 ). These applications, however, may be restricted by the well-known tendency of the MC isomer to undergo hydrolytic decomposition in aqueous solution, resulting in the formation of salicylaldehyde and a Fischer’s base derivative ( Stafforst and Hilvert, 2008 ; Hammarson et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Spiropyran-Based Drug Delivery Systems

2021

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Nanocarriers are rapidly growing in popularity in the field of drug delivery. The ability of nanocarriers to encapsulate and distribute poorly soluble drugs while minimising their undesired effects is significantly advantageous over traditional drug delivery. Nanocarriers can also be decorated with imaging moieties and targeting agents, further incrementing their functionality. Of recent interest as potential nanocarriers are spiropyrans; a family of photochromic molecular switches. Due to their multi-responsiveness to endo- and exogenous stimuli, and their intrinsic biocompatibility, they have been utilised in various drug delivery systems (DDSs) to date. In this review, we provide an overview of the developments in spiropyran-based DDSs. The benefits and drawbacks of utilising spiropyrans in drug delivery are assessed and an outline of spiropyran-based drug delivery systems is presented.

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Multicolor Fluorescence Photoswitching: Color‐Correlated versus Color‐Specific Switching

Kim

Park

2018

Advanced Optical Materials

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Fluorescence photoswitching systems using photochromic molecules, which turn on and off their fluorescence upon light irradiation, have emerged as highly promising material systems during the past two decades related to their optoelectronic applications such as high‐density optical memory, bioimaging, and super‐resolution microscopy. Single‐color fluorescence photoswitching, which provides only two different states (on/off), is limited in terms of its practical applications such as interference from autofluorescence in biological applications and limited switching states in logic gate and optical memory applications. To address such issues, studies on multicolor fluorescence photoswitching systems incorporating photochromic molecules have witnessed an explosive growth in the past decade in terms of the academic principles and technological applications. In the earlier part, this review briefly introduces the principle of fluorescence photoswitching based on the representative single‐color fluorescence photoswitching systems. Then, the review turns into the main topic of multicolor fluorescence photoswitching systems which are organized in two different subcategories of 1) color‐correlated photoswitching and 2) color‐specific photoswitching. Not only the material systems and principles of the multicolor fluorescence photoswitching, but also their important applications are described and discussed here. In the last section of this review, a brief summary and outlook on the future development are provided.

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FRET-based acrylic nanoparticles with dual-color photoswitchable properties in DU145 human prostate cancer cell line labeling

Cited by 36 publications

References 28 publications

Controlled Release and Photothermal Behavior of Multipurpose Nanocomposite Particles Containing Encapsulated Gold-Decorated Magnetite and 5-FU in Poly(lactide-co-glycolide)

Controlled Release and Photothermal Behavior of Multipurpose Nanocomposite Particles Containing Encapsulated Gold-Decorated Magnetite and 5-FU in Poly(lactide-co-glycolide)

Spiropyran-Based Drug Delivery Systems

Multicolor Fluorescence Photoswitching: Color‐Correlated versus Color‐Specific Switching

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