Freshwater environments as reservoirs of antibiotic resistant bacteria and their role in the dissemination of antibiotic resistance genes

Nnadozie, Chika Felicitas; Odume, Oghenekaro Nelson

doi:10.1016/j.envpol.2019.113067

Cited by 210 publications

(91 citation statements)

References 186 publications

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“…And also, the widespread of sul2 was due to the fact that it usually exists on small non-conjugative or large transmissible multi-resistant plasmids [47,48]. The abundance of sul1 and sul2 were usually related to input from WWTPs effluent discharge into freshwater and inputs from urban activities such as agricultural runoff, urban discharges and other human activities in previous studies [49,50], revealed that the water quality in this reservoir keep stable and no tendency of deterioration in recent years.…”

Section: Discussionmentioning

confidence: 85%

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Zhao

Guo

et al. 2020

Preprint

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Background Aquatic ecosystems are considered to be among the most important reservoirs of antibiotic resistance genes (ARGs). Drinking water sources were usually parts of lakes and rivers in Yangtze River Delta, among which Qingcaosha Reservoir is the largest river impoundment and benefit the population of more than 13 million for Shanghai city. In this study, we aimed at investigating the distribution of antibiotics and ARGs to characterize the pollution across various sites in Qingcaosha Reservoir in three seasons. Results Sulfamethoxazole, sulfamonomethoxine and penicillin G potassium salt were the dominant antibiotics and of high detection frequencies in this reservoir. Sulfonamide resistance genes ( sul1 and sul2 ) were the most prevalent and predominant genes. Higher total relative abundance of the ARGs were detected in the site closest to the inflow than those in other sites. Overall, the concentrations of antibiotics in May (spring) were relatively lower than November (autumn) and February (winter). Correlation analysis indicated sul1 , ermB and mphA had positive correlation with corresponding antibiotics in February and intI1 was also greatly positively correlated to sul1 , sul2 , ermB and mphA . Conclusion In conclusion, the antibiotics and ARGs were widespread in Qingcaosha Reservoir. Our result indicated that the drinking water reservoir might serve as gene reservoir for antibiotic resistance and mobile gene element intI1 can serve as a medium to contribute to the widespread of various ARGs. What is more, we considered that Reservoir could be served as a functional area contributing to the elimination of ARGs.

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Section: Discussionmentioning

confidence: 85%

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Zhao

Guo

et al. 2020

Preprint

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show abstract

“…And also, the widespread of sul2 was due to the fact that it usually exists on small non-conjugative or large transmissible multi-resistant plasmids [46,47]. The abundance of sul1 and sul2 were usually related to input from WWTPs effluent discharge into freshwater and inputs from urban activities such as agricultural runoff, urban discharges and other human activities in previous studies [48,49], revealed that the water quality in this reservoir is stable and showed no tendency of deterioration in recent years.…”

Section: Fig 5 Correlations Between Several Antibiotics and Args In mentioning

confidence: 83%

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Zhao

Guo

et al. 2020

Environ Sci Eur

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Background: Aquatic ecosystems are considered to be among the most important reservoirs of antibiotic resistance genes (ARGs). Drinking water sources could serve as critical points of ARGs transfer from environment to human. In this study, the distribution of antibiotics and ARGs across Qingcaosha Reservoir located in the Yangtze River Delta during three seasons was investigated to characterize the pollution patterns. Results: Sulfamethoxazole, sulfamonomethoxine and penicillin G potassium salt were the dominant antibiotics in this reservoir. Sulfonamide resistance genes (sul1 and sul2) were the most prevalent and predominant genes. Higher total relative abundance of the ARGs was detected in the site closest to the inflow than those in other sites. Overall, the concentrations of antibiotics in May were relatively lower than those in November and February. Correlation analysis indicated sul1, ermB and mphA had positive correlation with corresponding antibiotics in February and sul1, sul2, ermB and mphA was also positively correlated to intI1. Conclusion: In conclusion, antibiotics and ARGs were widespread in Qingcaosha Reservoir. Our results indicated that the drinking water reservoir might serve as gene reservoir for antibiotic resistance and mobile gene element intI1 can serve as a medium to contribute to the widespread of various ARGs. As the ARGs abundance was found to be higher in the inflow of the reservoir compared to the outflow, it is considered that reservoir could serve as a functional area contributing to the elimination or dilution of ARGs.

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“…These used antibiotics are not completely absorbed or metabolized by the body [5]. After being discharged, these antibiotics can pollute and spread in the environment through a variety of ways, such as agricultural runoff, sewage discharge and nearby farm leaching [6]. As a result, many symbiotic bacteria such as Escherichia coli have to live in the environment which is containing antibiotics for a long time, and this kind of environment can provide appropriate selection pressure for the emergence and spread of multi-antibiotic-resistant bacteria and antibioticresistant genes.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

Chen

Zhang

et al. 2020

Preprint

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Background: Sulfonamides is the second most popular antibiotic in many countries, which leads to the widespread emergence of sulfonamides resistance. Sul3 is a late sulfanilamide resistance gene, whose research is relatively little. Result: 46 sul3 positive E. coli strains were separated. A total of 12 ST types were observed, and 1 of those was previously unknown type. The ST350 is the most numerous type. All isolates were multidrug-resistant E. coli, with high antimicrobial rates to penicillin, ceftriaxone sodium, streptomycin, tetracycline, ciprofloxacin, gatifloxacin and chloramphenicol (100%, 73.9%, 82.6%, 100%, 80.4%, 71.7% and 97.8%), and with at least 3 resistance genes in addition to sul3. The plasmids transfered from 3 sul3-positive isolates to C600, the most of which brought 7 antibiotic resistance and increased resistance genes to C600. The transferred sul3 gene and the plasmid that carries it could be stably inherited in the recipient bacteria for at least 20 days. Those plasmids had no effect on the growth of the recipient bacteria, but it would greatly reduce (at least 60 time) the in vitro competitiveness of the strains. Conclusions: In Nanning, these sul3-positive Escherichia coli have strong antimicrobial resistance, and the plasmid carrying sul3 has the ability to transfer multiple resistance genes, so long-term monitoring is necessary. Since the transferred plasmid will greatly reduce the in vitro competitiveness of the strain, we can consider limiting the spread of antimicrobial in this respect.

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Freshwater environments as reservoirs of antibiotic resistant bacteria and their role in the dissemination of antibiotic resistance genes

Cited by 210 publications

References 186 publications

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China

Antimicrobial and Transconjugants Characteristics of Sul3 Positive Escherichia Coli Isolated from Animals in Nanning, Guangxi Province

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