Freshwater Decapods and Pesticides: An Unavoidable Relation in the Modern World

Negro, Leandro; Senkman, Eloisa; Montagna, Marcela C.; Collins, Pablo Agustín

doi:10.5772/16735

Cited by 11 publications

(13 citation statements)

References 84 publications

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“…However, gill FOX assay results were significantly decreased after exposure to 2,4-D. The unchanged FOX assay results in hepatopancreas and abdomen muscle in the present study are in agreement with the results of Sarikaya et al (47). Peroxidative effects observed in sublethal 2,4-D toxicity may cause damage in gills as an early response of cell/tissue damage seen in parallel with histology results.…”

Section: Biochemical Analysessupporting

confidence: 83%

“…In addition, diameters and numbers of B cells increased after being fed with hexachlorobenzen-contaminated Chlorella for three days. As known (47), exposure of crustaceans to pesticides can also lead to histopathological changes such as the interstitial sinus haemocytic infiltration, thickened and/or separated necrotic cells from the basal laminae, melanisation and coagulation in the thickened basal laminae, abnormal lumen of the tubules, necrotic tubules containing tissue debris, and haemocytes can constitute a wall around the thickened basal laminae of the tubules. Such severe histopathological changes were not observed in study.…”

Section: Histological Analysesmentioning

confidence: 99%

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The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

Benlı

Şahin

Sarıkaya

et al. 2016

Archives of Industrial Hygiene and Toxicology

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2,4-D is a widely used phenoxy herbicide, potentially toxic to humans and biota. The objective of the present study was to reveal short term sublethal effects of 2,4-D on narrow-clawed freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823), based on histology, total haemocyte counts, selected haemolymph parameters, and oxidative stress parameters. In the laboratory conditions crayfish specimens were exposed to 9 mg L -1 of 2,4-D for one week. Experiments were conducted under semi-static conditions in 20 L-capacity aquaria where 10 freshwater crayfish were stocked per aquarium. Exposure (experimental) and control groups were used and the experiments were repeated two times. No mortality and behavioural changes were recorded during the experiments. Total haemocyte counts decreased significantly, while haemolymph glucose levels increased (P<0.05), when compared to the control group. Haemolymph levels of calcium, chloride, sodium, potassium, magnesium, total protein, and lactate did not change. Exposure resulted with increased levels of malondialdehyde (MDA) only in hepatopancreas. However, results of gill FOX assay showed a significant decrease in oxidative stress parameters (P<0.05). MDA levels of gill and abdominal muscle tissues and FOX levels of hepatopancreas and abdominal muscle tissues did not change when compared to the control group. Significant histopathological alterations were observed both in hepatopancreas (multifocal deformations in tubule lumen) and gill tissue (melanisation of gill lamella). Exposure of crayfish even to a sublethal concentration of 2,4-D alters histopathology and lipid peroxidation due to stress. Biomarkers studied here seem to be useful for the assessment of adverse/toxic effects of pesticides on non-target, indicator aquatic organisms.KEY WORDS: haemolymph; herbicide; histology; oxidative stress; toxicity T h e f i r s t s u c c e s s f u l s e l e c t i v e h e r b i c i d e , 2,4-dichlorophenoxy acetic acid (2,4-D), was developed in 1946. It belongs to the phenoxy herbicide family and is still one of the systemic herbicides widely used to control many types of broadleaf weeds (1). It is used to control aquatic vegetation, in pasture and rangeland applications, in cultivated agriculture, forest management, home, and garden. It acts by sustaining high levels of the plant hormone auxin, which results in overstimulation of plant growth and death. In addition to this, it causes changes in the animal nervous system based on receptor interaction/interference of acetylcholine. Furthermore, it inhibits the acetyl cholinesterase (AChE) activity and increases the level of serotonin.Available data on 2,4-D toxicity on aquatic organisms mostly rely on the reports of its acute toxic effects observed in different fish species. Oruc and Uner (4) studied the combined effects of azinphosmethyl and 2,4-D on Oreochromis niloticus to clarify the mode of its action on the cellular defence system. Farah et al. (5) (8); for catfish (Clarias batrachus) by studying micronuclei and erythrocyte alt...

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Section: Biochemical Analysessupporting

confidence: 83%

Section: Histological Analysesmentioning

confidence: 99%

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

Benlı

Şahin

Sarıkaya

et al. 2016

Archives of Industrial Hygiene and Toxicology

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“…In aquatic organisms gills are vital organs, playing an important role in transporting respiratory gases, arranging osmoregulation and ionic change. Gills are also known as the first barriers for waterborne pollutants (Mallatt, ; Negro et al, ). In this study gill tissue exposed to etofenprox exhibited hyperplasia, lining in the afferent and efferent branchial vessels and hyperemia like structures.…”

Section: Discussionmentioning

confidence: 99%

The influence of etofenprox on narrow clawed crayfish (Astacus leptodactylus Eschscholtz, 1823): Acute toxicity and sublethal effects on histology, hemolymph parameters, and total hemocyte counts

Benlı

2014

Environmental Toxicology

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The acute and sublethal effects of etofenprox, a nonester pyrethroid, was determined in narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823). Semistatic bioassay procedures were followed in both experiments, and the 24, 48, 72, and 96 h LC50 values (with 95% confidence limits) of technical etofenprox for crayfish were calculated as 0.68, 0.61, 0.45, and 0.41 µg/L, respectively based on Finney's probit analysis. Two concentrations of etofenprox (0.04 and 0.1 µg/L) were tested to determine sublethal effects due to 96 hours exposure. After exposure to sublethal etofenprox, hemolymph glucose, and lactate levels increased while total hemocyte counts and sodium levels decreased (p < 0.05). Hemolymph calcium, potassium, magnesium, and chloride concentrations did not change significantly. Histological alterations were evident in the gills and hepatopancreas after exposure to sublethal etofenprox concentrations. Lamellar hyperplasia and lining in the afferent and efferent branchial vessels were recorded in gills; whilst tubule necrosis was obvious in hepatopancreas. Etofenprox was found to be very highly toxic to crayfish, a nontarget organism. Exposure to sublethal concentrations for 96 h affected circulating hemocytes and hemolymph stress parameters via histological response, to compansate for the adverse effects of etofenprox. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 887-894, 2015.

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“…Persistence of ChE inhibition several days after cessation of treatment with various organophosphate pesticides has been described in several species of crustaceans (Negro et al, 2011). This persistent inhibition enhances the vulnerability of organisms to other anthropogenic and natural stressors including further exposure to anti-lice pesticides.…”

Section: Effect Of Depurationmentioning

confidence: 98%

“…Exposure to sublethal concentrations (75% LC50) of the organophosphate pesticide, chlorpyrifos caused hyperactivity and increased oxygen consumption in the freshwater crab Barytelphusa guerini (Srivastava et al, 2013). In addition to causing AChE inhibition and altered neuromuscular function (Fulton and Key, 2001), organophosphate pesticides can also cause oxidative stress (Negro et al, 2011), gill damage (Lignot et al, 1997) and metabolic disturbances (Tu et al, 2010). Exposure to organophosphate pesticides prior to shipment therefore have the potential to impair the ability of lobster to cope with subsequent stressors and increase mortality during shipment, resulting in economic loss.…”

Section: Introductionmentioning

confidence: 99%

Sublethal exposure to azamethiphos causes neurotoxicity, altered energy allocation and high mortality during simulated live transport in American lobster

Couillard

Burridge

2015

Ecotoxicology and Environmental Safety

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Freshwater Decapods and Pesticides: An Unavoidable Relation in the Modern World

Cited by 11 publications

References 84 publications

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

The sublethal effects of (2,4-Dichlorophenoxy) acetic acid (2,4-D) on narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823)

The influence of etofenprox on narrow clawed crayfish (Astacus leptodactylus Eschscholtz, 1823): Acute toxicity and sublethal effects on histology, hemolymph parameters, and total hemocyte counts

Sublethal exposure to azamethiphos causes neurotoxicity, altered energy allocation and high mortality during simulated live transport in American lobster

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