Freshly-mixed and setting calcium-silicate cements stimulate human dental pulp cells

Pedano, Mariano Simón; Li, Xin; Li, Shu-Chen; Sun, Zeyi; Čokić, Stevan M.; Putzeys, Eveline; Yoshihara, Kazuhiro; Yoshida, Yashuhiro; Chen, Zhi; Landuyt, Kirsten Van; Meerbeek, Bart Van

doi:10.1016/j.dental.2018.02.005

Cited by 44 publications

(48 citation statements)

References 42 publications

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“…Although Biodentine was the most inert material tested, even at early time points there are reports of its initial high cytotoxicity because of the presence of calcium chloride in the liquid and/or the cement's higher initial pH (≈12) compared with other cements during the setting period . In the current study, the eluate was prepared after complete setting of the materials, thus explaining the favorable results of Biodentine.…”

Section: Discussionmentioning

confidence: 75%

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Ali

Mustafa

Bårdsen

et al. 2019

European J Oral Sciences

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Tricalcium silicate cements (TSCs) are used in endodontic procedures to promote wound healing and hard tissue formation. The aim of this study was to evaluate and compare the effect of commonly used TSCs [mineral trioxide aggregate (MTA), Biodentine, and TotalFill] on cellular metabolism and osteogenic/angiogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) in vitro. We tested the null hypothesis of no difference between MTA, Biodentine, and TotalFill in stem cell responses. Cells were subjected to eluates of the tested materials for up to 14 d. Cell viability was evaluated using the 3‐(4,5‐dimethyl‐thiazoyl)‐2,5‐diphenyl‐tetrazolium bromide (MTT) assay. Real‐time PCR was used to determine the levels of expression of the osteogenic factors alkaline phosphatase (ALP), osteoprotegerin (OPG), osteocalcin (OC), and collagen 1A (COL1A1), and the angiogenic factors vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1). ELISAs were used to measure the levels of VEGFA and ALP in culture supernatants. Mineralization in vitro of hBMSCs was assessed using Alizarin Red staining. The hBMSCs tolerated exposure to TSCs well, with Biodentine showing the most favorable effect on cell viability. Expression of ALP, COL1A1, OPG, and VEGFA were differentially affected by the materials, with Biodentine and TotalFill inducing earlier changes at gene level. Increased mineralization was observed with time, after exposure to all TSCs tested, with MTA showing the greatest effect. The results revealed different responses of hBMSCs to TSCs in vitro.

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Section: Discussionmentioning

confidence: 75%

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Ali

Mustafa

Bårdsen

et al. 2019

European J Oral Sciences

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“…While cell-culture laboratory studies are very useful for investigating some of the basic cell-interaction/response requirements of the materials, they do not provide clinical evidence of actual pulpcapping effectiveness. Moreover, most of the cell studies involve 2D-cell cultures, which have limitations because these cultures do not represent cell behavior in 3D structures [5][6][7]. On the other hand, animal studies are good models for testing pulp-capping agents, but the pulp-tissue T All teeth were rinsed in 70% ethanol (Hydral 70, VWR, Leuven, Belgium) for 1 min, followed by sterile Phosphate Buffered Saline (PBS; Sigma Aldrich, St. Louis, MO, USA) for 1 min and then placed in tooth-culture medium until they were used.…”

Section: Introductionmentioning

confidence: 99%

Survival of human dental pulp cells after 4-week culture in human tooth model

Pedano

Jeanneau

et al. 2019

Journal of Dentistry

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This study aimed to validate the human tooth model by investigating the growth efficiency, expression of mesenchymal stem cell (MSC) markers and differentiation ability of human dental pulp cells (hDPCs) harvested from extracted immature third molars and cultured for different periods. Moreover, the effect of exposure and capping with a hydraulic calcium-silicate cement on pulp tissue after 4-week culture in the tooth model was investigated. Methods: Primary hDPCs were collected from 18 molars from six individuals (15-19 years). One tooth of each patient was immediately cultured (control), while the other teeth were exposed to culture medium for 1, 2 or 4 weeks. After different culture periods, cells were harvested using the explant method, upon which cells were evaluated for cell-doubling time, colony-forming efficiency and expression of cell surface markers. The osteogenic, adipogenic and chondrogenic differentiation efficacy was also determined. Two teeth from three different patients (n = 6) were used for the pulp-capping assay. Three teeth were capped with ProRoot MTA (Dentsply Sirona), while three other exposed teeth remained uncapped (control). Results: Cells were found to grow, express MSC markers and showed osteogenic, adipogenic and chondrogenic differentiation potential at all time periods. Histology of the teeth subjected to the pulp-capping assay showed the formation of mineralized tissue after 4-week exposure to ProRoot MTA (Dentsply Sirona) and normal histological features in the control teeth. Conclusions: This study confirmed that hDPCs of teeth cultured for up to 4 weeks in a human tooth model are viable, express MSC markers and show differentiation ability. Clinical Significance: The human tooth model can be seen as an advanced cell-culture model that makes use of the original 3D pulp-chamber structure. It can serve as a screening tool to evaluate new pulp-capping formulations in a relatively cheap and fast manner.

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“…Calcium silicate-based materials have drawn attention in recent years due to their biocompatibility and clinical advantages, as revealed by numerous studies in the field of dentistr y [21][22][23][24][25][26][27][28]. A recent comparative study investigating the chemical composition and porosity characteristics of various calcium silicate-based cements concluded that, among them, Biodentine and Mineral Trioxide Aggregate (MTA) showed superior physicochemical properties [29].…”

Section: Resultsmentioning

confidence: 99%

Study of the Compatibility of New Biomaterials in Vital Pulp Therapy in Pediatric Dentistry

Todea¹,

Igna²,

Ogodescu³

et al. 2018

Rev. Chim.

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Vital pulp therapy is an expanding concept in pediatric dentistry today thanks to the potential exhibited by new biomaterials to stimulate regeneration of the pulp cells. Biocompatibility, bioactivity and dentin-like physical properties, as well as good handling, fast setting and the possibility to fill large cavities with a single material are some of the ideal properties of materials aimed to heal traumatized and inflamed dental pulps, given by a good balance of their chemical components.

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Freshly-mixed and setting calcium-silicate cements stimulate human dental pulp cells

Cited by 44 publications

References 42 publications

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Survival of human dental pulp cells after 4-week culture in human tooth model

Study of the Compatibility of New Biomaterials in Vital Pulp Therapy in Pediatric Dentistry

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